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pSuperRetro human Hic-5

Plasmid

#11188

Depositor

Bruce Spiegelman

Article

Drori et al Genes Dev. 2005 Feb 1. 19(3):362-75.

Insert

Hic-5 shRNA (TGFB1I1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pFS337-USP44-FLAG

Plasmid

#43905

Depositor

Stephen Elledge

Article

Stegmeier et al Nature. 2007 Apr 19;446(7138):876-81.

Insert

USP44 (USP44 Human)

Use

Baculoviral expression

Tags

FLAG

Expression

Insect

Available Since

April 1, 2013

Availability

Academic Institutions and Nonprofits only
pAC-EsaR-I70V

Plasmid

#47663

Purpose

pAC-EsaR with a208g mutation in esaR

Depositor

Cynthia Collins

Article

Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

Insert

esaR-I70V

Use

Synthetic Biology

Expression

Bacterial

Mutation

Changed Isoleucine 70 to Valine

Promoter

Plac

Available Since

Sept. 6, 2013

Availability

Academic Institutions and Nonprofits only
pR184

Plasmid

#47952

Purpose

rtTA positive feedback loop with three tet operators in the GAL1 promoter

Depositor

Attila Becskei

Article

Hsu et al Nat Commun. 2012 Feb 21;3:682. doi: 10.1

Insert

rtTA

Use

Tet inducible expression

Expression

Yeast

Promoter

Gal1

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pBa.NgCAM-Halo

Plasmid

#162719

Purpose

Mammalian expression of chicken NgCam

Depositor

Marvin Bentley

Article

Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

Insert

NgCam (L1CAM Chicken)

Tags

Halo

Expression

Mammalian

Mutation

E137K, K138E, and K642E

Promoter

Chicken Beta actin

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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MLM2579

Plasmid

#43891

Purpose

Activation Domain: NF-kB; RVD of C-terminal 0.5 TALE repeat: NG (binds T)

Depositor

Keith Joung

Article

Maeder et al Nat Methods. 2013 Feb 10. doi: 10.1038/n

Insert

TALE-activator p65 backbone

Use

Tale transcriptional activator expression vector

Promoter

EF1alpha

Available Since

March 8, 2013

Availability

Academic Institutions and Nonprofits only

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RRAS2

Plasmid

#55660

Purpose

expression of mouse RRAS2

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RRAS2 (Rras2 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pCS2-USP44

Plasmid

#43893

Depositor

Stephen Elledge

Article

Stegmeier et al Nature. 2007 Apr 19;446(7138):876-81.

Insert

USP44 (USP44 Human)

Expression

Mammalian

Promoter

SP6 or T7

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only
pZS*11-yfp0

Plasmid

#53241

Purpose

express yellow fluorescent protein (YFP) with strong T7 RBS at a low gene dosage (3-4 copies/cell)

Depositor

Philippe Cluzel

Article

Subramaniam et al Proc Natl Acad Sci U S A. 2013 Feb 5;110

Insert

YFP

Expression

Bacterial

Promoter

PLtetO1

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pN1-NLS-Q7mu

Plasmid

#129314

Purpose

Positive control for ATP measurement, nucleus

Depositor

Yasushi Okada

Article

Yaginuma et al Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038

Insert

QUEEN-7mu

Expression

Mammalian

Available Since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs-EGFP-Rheb SSVM-IP

Plasmid

#13832

Depositor

Shinya Yamanaka

Article

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

Rheb SSVM (Rheb Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

C181 changed to serine

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
pT2MP.EF-QNA

Plasmid

#129306

Purpose

Negative control for ATP measurement, Tol2 transposon

Depositor

Yasushi Okada

Article

Yaginuma et al Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038

Insert

QUEEN-NA

Expression

Mammalian

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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HA Raptor mutant 3

Plasmid

#8473

Depositor

David Sabatini

Article

Kim et al Cell 2002 Jul 26;110(2):163-75.

Insert

Raptor (RPTOR Human)

Tags

ha

Expression

Mammalian

Mutation

313NWIF316-AAAA (N313A, W314A, I315A, F316A)

Available Since

May 11, 2005

Availability

Academic Institutions and Nonprofits only
pMaCTag-18

Plasmid

#119997

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mKeima8.5

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mKeima8.5

Use

Pcr template

Tags

mKeima8.5

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pME-Gal80

Plasmid

#51620

Purpose

vertebrate expression of full-length Gal80

Depositor

Joshua Bonkowsky

Article

Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

Insert

Gal80 (GAL80 Budding Yeast)

Use

Vertebrate expression

Available Since

March 27, 2014

Availability

Academic Institutions and Nonprofits only

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pMT-GFP-SAK

Plasmid

#31755

Depositor

Ron Vale

Article

Goodwin et al Cell. 2010 Oct 15;143(2):263-74.

Insert

Sak (SAK Fly)

Tags

GFP

Expression

Insect

Mutation

A115S

Promoter

Metallothionein

Available Since

Jan. 20, 2012

Availability

Academic Institutions and Nonprofits only
pMT-GFP-Sas4

Plasmid

#31756

Depositor

Ron Vale

Article

Goodwin et al Cell. 2010 Oct 15;143(2):263-74.

Insert

Sas4 (Sas-4 Fly)

Tags

GFP

Expression

Insect

Mutation

N70T

Promoter

Metallothionein

Available Since

Jan. 20, 2012

Availability

Academic Institutions and Nonprofits only
pJEC578

Plasmid

#174366

Purpose

dCas9 for modular CRISPRa. SYNZIP-dCas9. Expresses dCas9 with a SYNZIP18 domain fused to the N terminus.

Depositor

James Chappell

Article

Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

Insert

dCas9 fused to SYNZIP18

Expression

Bacterial

Promoter

J23150

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pBMN-flag beta-catenin K49R

Plasmid

#44750

Depositor

Eric Fearon

Article

Winer et al J Biol Chem. 2006 Sep 8;281(36):26181-7.

Insert

beta-catenin

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

K49R

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only
phpRL-BCL2-FL-pA

Plasmid

#42595

Depositor

Richard Lloyd

Article

Sherrill et al J Biol Chem. 2004 Jul 9;279(28):29066-74

Insert

hairpin - renilla luciferase - BCL2 5'UTR - firefly luciferase

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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