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pcDNA4-AMBRA1-3xFLAG

Plasmid

#174157

Purpose

Vector for expression of 3xFLAG-tagged wild-type AMBRA1

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA1 (AMBRA1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLV x EF1a_EKAREN5(TA)-ires-puro

Plasmid

#167823

Purpose

control sensor for EKAREN5

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN5(TA)

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W, T420A

Promoter

EF1a

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLX304_zeo_empty

Plasmid

#160093

Purpose

Control lentiviral vector for mammalian gene expression

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) IGF1R

Plasmid

#107499

Purpose

Expresses IGF1R, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

IGF-1R (IGF1R Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pEB2-2ndVal mKate2

Plasmid

#104030

Purpose

Plasmid encoding 2ndVal mKate2

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal mKate2

Use

Low copy

Expression

Bacterial

Mutation

mKate2 with valine inserted in 2nd position

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pEB2-DsRed-Express

Plasmid

#103995

Purpose

Plasmid encoding DsRed-Express

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

DsRed-Express

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to DsRed (R2A, K5E, N6D, T21S,…

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pEB1-mCerulean ME

Plasmid

#103971

Purpose

Plasmid encoding mCerulean ME

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mCerulean ME

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, S65T, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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hgRNA-A21_pLKO-Puro

Plasmid

#100570

Purpose

hgRNA-A21 in a lentiviral backbone with puromycin resistance

Depositor

George Church

Article

Kalhor et al Nat Methods. 2017 Feb;14(2):195-200. doi

Insert

hgRNA-A21

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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p414ADH_Hsp82_CycTER

Plasmid

#61712

Purpose

yeast plasmid with Hsp82

Depositor

Dan Bolon

Article

Jiang et al PLoS Genet. 2013 Jun;9(6):e1003600. doi:

Insert

6xHis-Hsp82 (HSP82 Budding Yeast)

Tags

6xHis

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pGL3-pomMx1

Plasmid

#30536

Purpose

Reporter (luciferase) for interferon signalling in fish

Depositor

Bertrand Collet, Christopher Secombes

Article

Collet et al Dev Comp Immunol. 2004 Jun . 28(7-8):793

Insert

rainbow trout Mx 1 promoter

Use

Luciferase

Expression

Mammalian

Available Since

May 13, 2014

Availability

Academic Institutions and Nonprofits only

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sgRNA with rpr-1 promoter

Plasmid

#48961

Purpose

to drive the sgRNA expression under RNase P non-coding RNA promoter

Depositor

Mario de Bono

Article

Chen et al Nucleic Acids Res. 2013 Sep 5.

Type

Empty backbone

Use

CRISPR

Expression

Worm

Promoter

rpr-1 promoter

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

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pDEST27-GST-ATXN1 FL(2Q)

Plasmid

#21752

Depositor

Huda Zoghbi

Article

Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

Insert

Ataxin-1 (ATXN1 Human)

Tags

GST

Expression

Mammalian

Mutation

insert contains 2Q (2 glutamines) and there isno …

Promoter

CMV

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only
pTH353-SUP45wt

Plasmid

#29371

Depositor

Tobias von der Haar

Article

Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

Insert

SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)

Expression

Yeast

Mutation

Wild type gene including genomic sequence 1432 bp…

Available Since

May 5, 2011

Availability

Academic Institutions and Nonprofits only

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pPS1739

Plasmid

#8922

Depositor

Pamela Silver

Article

Ferrigno et al EMBO J. 1998 Oct 1. 17(19):5606-14.

Insert

HOG1 (HOG1 Budding Yeast)

Tags

GFP

Expression

Yeast

Available Since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only
pAc5.1B-lambdaN-HA-DmLSm2_A

Plasmid

#145887

Purpose

Insect Expression of DmLSm2

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLSm2 (CG10418 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmLSm5_A

Plasmid

#145890

Purpose

Insect Expression of DmLsm5

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLsm5 (CG6610 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmLSm7_A

Plasmid

#145891

Purpose

Insect Expression of DmLSm7

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLSm7 (LSm7 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmLSm3_A

Plasmid

#145888

Purpose

Insect Expression of DmLSm3

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLSm3 (LSm3 Fly)

Expression

Insect

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Hmga2 shMut wt

Plasmid

#52036

Purpose

encodes shMut HMGA2

Depositor

Julian Downward

Article

Kumar et al Nature. 2014 Jan 9;505(7482):212-7. doi:

Insert

Hmga2 (Hmga2 Mouse)

Expression

Mammalian

Mutation

shRNA binding site mutated

Promoter

CMV

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Hmga2 shMut m7

Plasmid

#52037

Purpose

encodes shMut HMGA2 m7

Depositor

Julian Downward

Article

Kumar et al Nature. 2014 Jan 9;505(7482):212-7. doi:

Insert

Hmga2 (Hmga2 Mouse)

Expression

Mammalian

Mutation

shRNA binding site mutated, all seven let-7 sites…

Promoter

CMV

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only