We narrowed to 82,808 results for: TRI

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pHS26_10xHis_MBP_TEV_VRER-Cas9

Plasmid

#244829

Purpose

Bacterial expression of VRER Cas9 for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

VRER Cas9

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Mutation

D1135V/G1218R/R1335E/T1337R

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHS16_10xHis_MBP_TEV_xPBA-Cas9

Plasmid

#244830

Purpose

Bacterial expression of xPBA Cas9 for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

xPBA Cas9

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHS23_10xHis_MBP_TEV_SpG-Cas9

Plasmid

#244826

Purpose

Bacterial expression of SpG Cas9 for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpG Cas9

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Mutation

D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHS25_10xHis_MBP_TEV_EQR-Cas9

Plasmid

#244828

Purpose

Bacterial expression of EQR Cas9 for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

EQR Cas9

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Mutation

D1135E/R1335Q/T1337R

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-RNF146(92-168)-EGFP

Plasmid

#196228

Purpose

EGFP fused to the C-terminus of a WWE domain within RNF146 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

RNF146(92-168)-EGFP

Use

Lentiviral

Tags

EGFP and Myc tag

Expression

Mammalian

Promoter

EF1A

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a asyn(66-140) E83A

Plasmid

#240583

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

Insert

aSyn (SNCA Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 66-140 only, E83A

Promoter

T7

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a asyn(66-140) K80A

Plasmid

#240584

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

Insert

aSyn (SNCA Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 66-140 only, K80A

Promoter

T7

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pT7-7 asyn K97A

Plasmid

#240601

Purpose

Overexpress mutant alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

Insert

aSyn (SNCA Human)

Expression

Bacterial

Mutation

K97A

Promoter

T7

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a asyn(66-140) K96A

Plasmid

#240585

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

Insert

aSyn (SNCA Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 66-140 only, K96A

Promoter

T7

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pT7-7 asyn K96A

Plasmid

#240600

Purpose

Overexpress mutant alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

Insert

aSyn (SNCA Human)

Expression

Bacterial

Mutation

K96A

Promoter

T7

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a asyn(66-140) K97A

Plasmid

#240586

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

Insert

aSyn (SNCA Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 66-140 only, K97A

Promoter

T7

Available Since

Aug. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a asyn(66-140) K102A

Plasmid

#240588

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

Insert

aSyn (SNCA Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 66-140 only, K102A

Promoter

T7

Available Since

Aug. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a asyn(66-140) D98A

Plasmid

#240587

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

Insert

aSyn (SNCA Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 66-140 only, D98A

Promoter

T7

Available Since

Aug. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pT7-7 asyn K80A

Plasmid

#240599

Purpose

Overexpress mutant alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

Insert

aSyn (SNCA Human)

Expression

Bacterial

Mutation

K80A

Promoter

T7

Available Since

Aug. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pAX1_Z11 gpN:SpyTag

Plasmid

#225196

Purpose

Used for adding C-terminal SpyTag to the Plesiomonas ZOR0011 P2 phage major capsid protein

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

gpN homology arms and SpyTag

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAX1_HS∆tum

Plasmid

#225198

Purpose

Used to create a markerless deletion of the E. coli HS tum gene

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

tum homology arms

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAX1_HS_lexA(Ind-)

Plasmid

#225199

Purpose

Used to create a non-inducible lexA by introducing a G85D mutation in E. coli HS

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

lexA homology arms

Expression

Bacterial

Mutation

lexA G85D

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAX1_HS_gpN:SpyTag

Plasmid

#225193

Purpose

Used for adding C-terminal SpyTag to the E. coli HS P2 phage major capsid protein

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

gpN homology arms and SpyTag

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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p25.pA.NGFR.mCMV.hPGK.Rab35(WT).wpre[sequenced]

Plasmid

#226764

Purpose

rab35 (WT)

Depositor

Ferdinando Pucci

Article

Ziglari et al Cancer Res. 2025 Mar 3;85(5):859-874. do

Insert

rab35 (RAB35 )

Expression

Mammalian

Available Since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR4 HopU1

Plasmid

#228395

Purpose

pENTR4 plasmid with CDS of Pseudomonas syringae pv tomato type III effector HopU1 without stop codon for C-terminal epitope tags

Depositor

Lennart Wirthmueller

Article

Simranjit et al bioRxiv 2023.09.25.558804

Insert

HopU1

Use

Gateway-compatible entry vector

Mutation

Alanine inserted after start Methionine due to Nc…

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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