217,374 results

1. pcDNA3.1/hChR2(H134R)-EYFP

   Plasmid

   #20940

   Purpose

   Mammalian expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Expression

   Mammalian

   Mutation

   H134R mutation in ChR2

   Promoter

   CMV

   Available Since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1/hChR2(H134R)-EYFP

   Plasmid

   #20940

   Purpose

   Mammalian expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Expression

   Mammalian

   Mutation

   H134R mutation in ChR2

   Promoter

   CMV

   Available Since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSRKBB-empty

   Plasmid

   #59449

   Purpose

   Contains BioBrick prefixed and suffixed insert with a modified lac promoter, Shine-Delgarno sequence, 5’ & 3’ MCS, 3’ 6xHis tag.

   Depositor

   Claudia Schmidt-dannert

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   modified lac

   Available Since

   Sept. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSRKBB-empty

   Plasmid

   #59449

   Purpose

   Contains BioBrick prefixed and suffixed insert with a modified lac promoter, Shine-Delgarno sequence, 5’ & 3’ MCS, 3’ 6xHis tag.

   Depositor

   Claudia Schmidt-dannert

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   modified lac

   Available Since

   Sept. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. EF1a-MVPH

   Plasmid

   #235594

   Purpose

   Expresses the MS2-VP64-P65-HSF1 under the control of human EF1a promoter

   Depositor

   Ting Zhou

   Article

   Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

   Insert

   MS2-VP64-P65-HSF1
   

   Expression

   Mammalian

   Mutation

   N55K in MS2

   Promoter

   EF1a

   Available Since

   May 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pORTMAGE-Ec1

   Plasmid

   #138474

   Purpose

   Enables high-efficiency recombineering in E. coli

   Depositor

   George Church

   Article

   Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

   Inserts

   CspRecT
   mutL E32K
   

   Expression

   Bacterial

   Mutation

   E32K

   Available Since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-PE7-P2A-hMLH1dn

   Plasmid

   #222997

   Purpose

   Mammalian expression of SpCas9 PE7 prime editor with P2A-human MLH1dn (codon optimized)

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7-P2A-hMLH1dn
   

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Deletion of residues 754-756 in MLH1 as described…

   Promoter

   CMV

   Available Since

   Aug. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRIS-PITChv2-BSD-dTAG (BRD4)

   Plasmid

   #91792

   Purpose

   PITCh dTAG donor vector for BSD-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.

   Depositor

   James Bradner, Behnam Nabet

   Article

   Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

   Insert

   BSD-P2A-2xHA-FKBP_F36V
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Phenylalanine 36 to valine in FKBP12

   Promoter

   Promoterless

   Available Since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-HDAC6-FLAG

   Plasmid

   #30482

   Depositor

   Tso-Pang Yao

   Article

   Kawaguchi et al Cell. 2003 Dec 12. 115(6):727-38.

   Insert

   HDAC6 (HDAC6 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-HDAC6-FLAG

    Plasmid

    #30482

    Depositor

    Tso-Pang Yao

    Article

    Kawaguchi et al Cell. 2003 Dec 12. 115(6):727-38.

    Insert

    HDAC6 (HDAC6 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge