We narrowed to 44,318 results for: tra
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Plasmid#208143PurposeAiE0390h_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated transgene expression in brain astrocytesDepositorInsertSYFP2
UseAAVMutationNAPromoterBeta Globin minimal promoterAvailable SinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2159
Plasmid#143347PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-ZBTB16
Plasmid#192903PurposeBarcoded piggybac transposon vector with Dox-inducible expression of ZBTB16DepositorAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-TCF21
Plasmid#192894PurposeBarcoded piggybac transposon vector with Dox-inducible expression of TCF21DepositorAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQ79
Plasmid#175064Purposeexpresses mutant of the huntingtin exon1 in mammalian cellsDepositorInsertpolyQ79 (HTT Human)
ExpressionMammalianMutationmVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…PromoterCMV PromoterAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Puro PRMT5-CRISPRi
Plasmid#164637PurposelentiGuide-Puro backbone with cloned target sequence for human PRMT5. The insert is: 5´- CACCGAGCCGCGTGTCCAGCGGGA-3. sgRNA achieves a downregulation of PRMT5 in combination with dCAS9-KRAB-MCP2DepositorInserttarget guide sequence PRMT5 inhibition (PRMT5 Human)
UseCRISPR and Lentiviral; InterferenceExpressionMammalianPromoterU6 and EF1AAvailable SinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
AiP11244 - pAAV-hDLXI56i-minBG-SYFP2-WPRE3-BGHpA (Alias: CN1244)
Plasmid#163493PurposeDirect-expressing SYFP2 AAV VirusDepositorInsertSYFP2
UseAAVPromoterminBetaGlobinAvailable SinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
AiP11408-pAAV-AiE0064h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN1408)
Plasmid#163500PurposeAiE0064h is an enhancer sequence designed to drive AAV-mediated transgene expression in Pvalb+ and Sst+ cortical interneuronsDepositorInsertSYFP2
UseAAVPromoterminBetaGlobinAvailable SinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Zhou-COVID-19-Spike
Plasmid#161029PurposeExtracellular domain of SARS-CoV-2 spike protein that has been engineered so that it assembles pseudo virus more efficiently. Also contains portions of VSV-G for production of psuedovirus.DepositorInsertEngineered COVID-19 Spike protein, VSV-G (S SAR-CoV-2, Vesicular stomatitis virus)
ExpressionMammalianMutationCodon optimizedAvailable SinceJan. 12, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
ePB-PURO-FLAG-METTL3-APPA
Plasmid#160253PurposeFor stable integration of catalytic inactive FLAG-METTL3 in human cells with trasposaseDepositorInsertmethyltransferase like 3 (METTL3 Human)
TagsFLAGExpressionMammalianMutationchanges in aa 395–398, DPPW → APPAPromotertight TRE promoterAvailable SinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRG713_lexO-HO_LexA-TF_URA3MX
Plasmid#154821PurposeInducible expression of HO endonuclease in S. cerevisiaeDepositorInsertsTagsFLAGExpressionYeastPromoterACT1 and lexO_4-CYC1_coreAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
AC50 pEGFPN1 anti-AMIGO-1 nanobody
Plasmid#135225PurposeMammalian expression of anti-AMIGO-1 (Mouse) recombinant llama nanobody for use as an intrabodyDepositorInsertanti-AMIGO-1 (Mouse) recombinant llama nanobody AC50 (Amigo1 L. glama (llama))
Tags6xHis-HA-EGFPExpressionMammalianAvailable SinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3XFLAG-TAK1-K63A
Plasmid#43933DepositorInsertTransforming growth factor-beta-activated kinase 1 (Map3k7 Mouse)
Tags3XFLAGExpressionMammalianMutationK63AAvailable SinceOct. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSKC12 - DelQ
Plasmid#110493PurposeMonitor the expression of Luciferase driven by a mutated NRAS 5'-UTR.DepositorInsertNRAS 5'- DelQ (bp 30-254) (NRAS Human)
UseLuciferaseTagsFirefly luciferaseMutationDeletion of the first 29 base pairs of the NRAS 5…PromoterT7Available SinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cherry-HA-KCC2
Plasmid#104077PurposeTo visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)DepositorInsertKCC2 (b isoform) (Slc12a5 Rat)
TagsmCherry-HA tagExpressionMammalianMutationEcoR1 site in rat KCC2 was removed by silent muta…PromoterUbCAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
hsDCX-pHAT
Plasmid#83928PurposehsDCX-pHAT N-terminal 6xHis-tag, C-terminal Strep-tagIIDepositorAvailable SinceDec. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
Plxna1-AP-His
Plasmid#71996PurposeExpresses the extracellular region of the PlexinA1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGFP-Kif5cmut
Plasmid#71854Purposemammalian expression of mutant GFP-Kif5cDepositorInsertKif5c (Kif5c Rat)
TagsEGFPExpressionMammalianMutationT93N mutation and also a novel SpeI site (1652). …PromoterCMVAvailable SinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HsSENP3(307-574)
Plasmid#71481PurposeEncodes the catalytic domain of human SENP3. Suitable for in vitro transcription/translation (T7).DepositorInsertSUMO1/sentrin specific peptidase 3 (SENP3 Human)
TagsHexahistidineExpressionBacterialMutationDeleted amino acids 1-306 (catalytic domain only)PromoterT7Available SinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCardinal-H3-23
Plasmid#56163PurposeLocalization: Nucleus/Histones, Excitation: 604, Emission: 659DepositorAvailable SinceFeb. 27, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits