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We narrowed to 3,124 results for: CCS

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Showing: 251 - 300 of 3124 results

  1. pACCS3-1

    Plasmid
    #78559
    Purpose
    4.7 kb HindIII ETEC CS3 fimbrial operon from pCS100 cloned into pACYC184.
    Depositor
    James Kaper
    Article
    Giron et al Vaccine. 1995 Jul;13(10):939-46.
    Insert
    cs3 operon
    Expression
    Bacterial
    Promoter
    tet
    Available Since
    June 22, 2016
    Availability
    Academic Institutions and Nonprofits only

  4. pEGFP-Rab21-CCSS

    Plasmid
    #83424
    Purpose
    Mouse Rab21, mutation of the putative COOH-terminal prenylation motif (residues 218 and 219 from C to S). Generated from pEGFP-Rab21 by site-directed mutagenesis.
    Depositor
    Johanna Ivaska
    Article
    Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.
    Insert
    Rab21 (Rab21 Mouse)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Residues 218 and 219 from C to S (CCSS: TGCTGT -&…
    Promoter
    CMV
    Available Since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    83424-plasmid-map-sequence-id-166315

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  8. eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA

    Plasmid
    #86613
    Purpose
    Vector for tandem expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G3 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via hdr using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available Since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86613-plasmid-map-sequence-id-168851

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  16. eSpCas9(1.1)_No_FLAG_ATP1A1_G2_Dual_sgRNA

    Plasmid
    #86612
    Purpose
    Vector for tandem expression of ATP1A1 G2 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G2 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via nhej using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available Since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86612-plasmid-map-sequence-id-168849

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  21. pTwist-SARS-CoV-2 Δ18 B.1.1.529

    Plasmid
    #179907
    Purpose
    Encodes SARS-CoV-2 B.1.1.529 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Garcia-Beltran et al Cell. 2022 Jan 6. pii: S0092-8674(21)014
    Insert
    SARS-CoV-2 B.1.1.529 Spike truncated to remove 18 amino acids (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    A67V; Δ69-70; T95I; G142D; Δ143-145; N211I; Δ212;…
    Promoter
    CAG
    Available Since
    Jan. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    179907-plasmid-map-sequence-id-351282

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  27. pSBBi-RP PcCS

    Plasmid
    #139159
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains Paullinia cupana caffeine synthase (PcCS); the other side contains RFP and puromycin resistance genes.
    Depositor
    Neal Devaraj
    Insert
    Caffeine Synthase [Paullinia cupana var. sorbilis]
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1α
    Availability
    Academic Institutions and Nonprofits only
    139159-plasmid-map-sequence-id-309125

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  28. pSBBi-RP CCS1

    Plasmid
    #139162
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains coffee caffeine synthase 1 (CCS1); the other side contains RFP and puromycin resistance genes.
    Depositor
    Neal Devaraj
    Insert
    caffeine synthase 1 [Coffea arabica]
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1α
    Availability
    Academic Institutions and Nonprofits only
    139162-plasmid-map-sequence-id-309352

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  29. pSBBi-RP CsAncCS

    Plasmid
    #139167
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains Camellia sinensis ancestral caffeine synthase (CsAncCS); the other side contains RFP and puromycin resistance genes.
    Depositor
    Neal Devaraj
    Insert
    Predicted Camellia sinensis Ancestral Caffeine Synthase
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1α
    Availability
    Academic Institutions and Nonprofits only
    139167-plasmid-map-sequence-id-309357

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  30. pSBBi-RP PcAncCS2

    Plasmid
    #139170
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains Paullinia cupana ancestral Caffeine Synthase 2 (PcAncCS2); the other side contains RFP and puromycin resistance genes.
    Depositor
    Neal Devaraj
    Insert
    Predicted Paullinia cupana var sobilis Ancenstral Caffeine Synthase-2
    Use
    Transposon
    Tags
    None
    Expression
    Mammalian
    Availability
    Academic Institutions and Nonprofits only
    139170-plasmid-map-sequence-id-309360

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  39. pSBBi-RP GST-PcCS

    Plasmid
    #139160
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains N-GST Paullinia cupana caffeine synthase; the other side contains RFP and puromycin resistance genes.
    Depositor
    Neal Devaraj
    Insert
    N-GST caffeine Synthase [Paullinia cupana var. sorbilis]
    Use
    Transposon
    Tags
    glutathione transferase
    Expression
    Mammalian
    Promoter
    EF1α
    Availability
    Academic Institutions and Nonprofits only
    139160-plasmid-map-sequence-id-309126

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  40. pSBBi-RP GST-CCS1

    Plasmid
    #139164
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains N-GST coffee caffeine synthase 1 (GST-CCS1) fusion; the other side contains RFP and puromycin resistance genes.
    Depositor
    Neal Devaraj
    Insert
    glutathione transferase + caffeine synthase 1 [Coffea arabica]
    Use
    Transposon
    Tags
    Glutathione transferase
    Expression
    Mammalian
    Promoter
    EF1α
    Availability
    Academic Institutions and Nonprofits only
    139164-plasmid-map-sequence-id-309354

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  45. pSB-Bleo Gal4 CCS1

    Plasmid
    #139189
    Purpose
    SB-transposon with constitutive bleomycin resistance and Gal4-inducible coffee caffeine synthase 1 (CCS1).
    Depositor
    Neal Devaraj
    Insert
    caffeine synthase 1 [Coffea arabica]
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    Gal4-inducible CMV and STAT3
    Availability
    Academic Institutions and Nonprofits only
    139189-plasmid-map-sequence-id-309396

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Showing: 251 - 300 of 3124 results