We narrowed to 9,822 results for: Coli

1. pET28b – E. coli RecE residues 564-868

   Plasmid

   #104534

   Purpose

   Expresses E. coli RecE residues 564-868

   Depositor

   Charles Bell

   Article

   Zhang et al Structure. 2009 May 13;17(5):690-702. do

   Insert

   RecE residues 564-866 (C-terminal nuclease domain)
   

   Tags

   6X-His tag followed by thrombin site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. MIP 339 CoMiP coLin28 2A Nanog IRES eGFP

   Plasmid

   #63729

   Purpose

   To express codon optimized Lin-28 and wild type Nanog. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).

   Depositor

   Mark Kay, Joseph Wu

   Article

   Diecke et al Sci Rep. 2015 Jan 28;5:8081. doi: 10.103

   Inserts

   Lin28 (LIN28A Human)
   Nanog (NANOG Human)
   

   Tags

   T2A

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   SFFV

   Available Since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAV-U6+27-Tornado-F30-Broccoli-Squash-SAM-sensor 4-2

   Plasmid

   #177919

   Purpose

   Expresses ratiometric Squash-SAM sensor 4-2 as circular RNA in mammalian cells using the Tornado expression system.

   Depositor

   Samie Jaffrey

   Article

   Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

   Insert

   Tornado-F30-Broccoli-Squash-SAM-sensor 4-2
   

   Expression

   Mammalian

   Promoter

   U6+27

   Available Since

   Feb. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAV-U6+27-Tornado-F30-Broccoli-Squash-SAM-sensor 5-1

   Plasmid

   #177920

   Purpose

   Expresses ratiometric Squash-SAM sensor 5-1 as circular RNA in mammalian cells using the Tornado expression system.

   Depositor

   Samie Jaffrey

   Article

   Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

   Insert

   Tornado-F30-Broccoli-Squash-SAM-sensor 5-1
   

   Expression

   Mammalian

   Promoter

   U6+27

   Available Since

   Feb. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. E. coli expression: Z-lock cofilin dark state mutant

   Plasmid

   #141135

   Purpose

   a control for optogenetic cofilin analog

   Depositor

   Klaus Hahn

   Article

   Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

   Insert

   Zdk2, Cofilin, LOV2
   

   Expression

   Bacterial

   Mutation

   D3 deletion, I32F in Zdk2

   Available Since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET E. coli expression vector with BioBrick polypromoter restriction sites (14-A)

   Plasmid

   #48307

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Nov. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato (AAV8)

   Viral Prep

   #60658-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato (#60658). In addition to the viral particles, you will also receive purified pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato plasmid DNA. CAG-driven, Flp-dependent expression of the voltage-gated Na+ channel mNaChBac-T2A-tdTomato. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Massimo Scanziani

   Article

   Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

   Promoter

   EF1α

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

8. Anti-DNA-directed RNA polymerase subunit beta' [PR-mAb NT73] (Recombinant Antibody)

   Antibody

   #203884-rAb

   Purpose

   Anti-DNA-directed RNA polymerase subunit beta' (E. coli) recombinant mouse monoclonal antibody

   Depositor

   Melina Fan

   Article

   Thompson et al Biochemistry. 1992 Aug 4;31(30):7003-8.

   Recommended Applications

   Western Blot

   Source Species

   Mouse

   Isotype

   IgG2a

   Trial Size

   Available to purchase

   Available Since

   Nov. 8, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

9. pAAV-syn-flex-iGluSnFR4s-NGR-WPRE (AAV1)

   Viral Prep

   #234441-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV-syn-flex-iGluSnFR4s-NGR-WPRE (#234441). In addition to the viral particles, you will also receive purified pAAV-syn-flex-iGluSnFR4s-NGR-WPRE plasmid DNA. Syn-driven, iGluSnFR4s-NGR sensor expression. These AAV preparations are suitable purity for injection into animals.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Promoter

   Syn

   Available Since

   Sept. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

10. pAAV-syn-iGluSnFR4f-NGR-WPRE (AAV1)

    Viral Prep

    #234438-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-syn-iGluSnFR4f-NGR-WPRE (#234438). In addition to the viral particles, you will also receive purified pAAV-syn-iGluSnFR4f-NGR-WPRE plasmid DNA. Syn-driven, iGluSnFR4f-NGR sensor expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Promoter

    Syn

    Available Since

    Sept. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

11. pAAV-syn-flex-iGluSnFR4f-NGR-WPRE (AAV1)

    Viral Prep

    #234442-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-syn-flex-iGluSnFR4f-NGR-WPRE (#234442). In addition to the viral particles, you will also receive purified pAAV-syn-flex-iGluSnFR4f-NGR-WPRE plasmid DNA. Syn-driven, iGluSnFR4f-NGR sensor expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Promoter

    Syn

    Available Since

    Sept. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

12. pAAV-syn-iGluSnFR4s-NGR-WPRE (AAV1)

    Viral Prep

    #234437-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-syn-iGluSnFR4s-NGR-WPRE (#234437). In addition to the viral particles, you will also receive purified pAAV-syn-iGluSnFR4s-NGR-WPRE plasmid DNA. Syn-driven, iGluSnFR4s-NGR sensor expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Promoter

    Syn

    Available Since

    Oct. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

13. Anti-GST [N100/13R ] (Recombinant Antibody)

    Antibody

    #192032-rAb

    Purpose

    Anti-GST recombinant mouse monoclonal antibody

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Recommended Applications

    Western Blot

    Reactivity

    Synthetic

    Source Species

    Mouse

    Isotype

    IgG2a

    Trial Size

    Available to purchase

    Available Since

    Jan. 27, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

14. psgRNA Library

    Pooled Library

    #115927

    Purpose

    ~93,000 gRNAs targeting random positions along the genome of E. coli MG1655 with a NGG PAM motif. Contains ~19 targets per gene and can be used with SpdCas9 to perform pooled CRISPRi screens.

    Depositor

    David Bikard

    Article

    Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

    Expression

    Bacterial

    Species

    Escherichia coli

    Use

    CRISPR

    Available Since

    June 6, 2019

    Availability

    Academic Institutions and Nonprofits only

15. pTXB1-FIS

    Plasmid

    #51564

    Purpose

    Expression of E. coli FIS for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone

    Depositor

    Sankar Adhya, Francisco Malagon

    Insert

    FIS
    

    Tags

    Intein-Chiting Binding Domanin

    Expression

    Bacterial

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTXB1-CRP/CAP

    Plasmid

    #51563

    Purpose

    Expression of E. coli CRP/CAP for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone

    Depositor

    Sankar Adhya, Francisco Malagon

    Insert

    CRP/CAP
    

    Tags

    Intein-Chiting Binding Domanin

    Expression

    Bacterial

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pETM41-EcPPK

    Plasmid

    #38334

    Depositor

    Florian Freimoser

    Insert

    E. coli polyphosphate kinase (ppk Escherichia coli)
    

    Tags

    6xHIS, MBP, and TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pKM263-EcPPK

    Plasmid

    #38333

    Depositor

    Florian Freimoser

    Insert

    E. coli polyphosphate kinase (ppk Escherichia coli)
    

    Tags

    6xHIS, GST, and TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. psgRNA_EcoWG1

    Pooled Library

    #131625

    Purpose

    Bacterial inhibition library targeting every small RNA and ORF of the E. coli MG1655 genome. This library has higher inhibition efficiency than the psgRNA library.

    Depositor

    David Bikard

    Article

    Calvo-Villamanan et al Nucleic Acids Res. 2020 Apr 30. pii: 582

    Expression

    Bacterial

    Species

    Escherichia coli

    Use

    CRISPR

    Available Since

    May 12, 2020

    Availability

    Academic Institutions and Nonprofits only

20. pCDM4-GLY

    Plasmid

    #49808

    Purpose

    Encodes pgk, gapA, aceE, aceF, lpdA in pseudo-operon configuration.

    Depositor

    Mattheos Koffas

    Article

    Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

    Inserts

    phosphoglycerate kinase
    glyceraldehyde-3-phosphate dehydrogenase
    pyruvate dehydrogenase subunit E1
    dihydrolipoamide acetyltransferase
    dihydrolipoamide dehydrogenase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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