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We narrowed to 116 results for: PRHA

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pCas3-Apr

Plasmid

#208000

Purpose

Expresses all necessary components of Type I-C CRISPR-Cas system

Depositor

Pablo Ivan Nikel

Article

Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

Inserts

RhaR (rhaR )
RhaS (rhaS )
pRhaBAD
I-C crRNA
Cas3
Cas5
Cas8

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pSLiP-G1G2

Plasmid

#188965

Purpose

L-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdown

Depositor

Seung-Goo Lee

Article

Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

Inserts

dCas9
sgRNA 1: atcggtcgcattgttttccactagg, sgRNA 2: gttagacgctgattacatggactagg

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PrhaBAD

Available since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCK353

Plasmid

#129697

Purpose

For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120015170

Depositor

John Heap

Article

Kelly et al Microorganisms. 2019 Aug 16;7(8). pii: m

Inserts

PrhaBAD
yfp
rhaS
Terminator ECK120015170

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCK355

Plasmid

#129699

Purpose

For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + ilvBN terminator

Depositor

John Heap

Article

Kelly et al Microorganisms. 2019 Aug 16;7(8). pii: m

Inserts

PrhaBAD
yfp
rhaS
ilvBN terminator

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCas3cRh

Plasmid

#133773

Purpose

Expresses all necessary components of Type I-C CRISPR-Cas system

Depositor

Joseph Bondy-Denomy

Article

Csorgo et al Nat Methods. 2020 Oct 19. pii: 10.1038/s

Inserts

RhaR (rhaR )
RhaS (rhaS )
pRhaBAD
I-C crRNA
Cas3
Cas5
Cas8
Cas7

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCK314

Plasmid

#110545

Purpose

Modified (delta CRP-binding site) rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria

Depositor

John Heap

Article

Kelly et al ACS Synth Biol. 2018 Apr 2. doi: 10.1021

Inserts

Modified PrhaBAD (CRP-binding sites removed)
yfp
rhaS

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pLY164

Plasmid

#184140

Purpose

Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 3)

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Inserts

dCas9
tetR
pspFΔHTH::λN22plus
sfgfp
rhaS

Use

Synthetic Biology

Tags

ASV tag and λN22plus

Expression

Bacterial

Mutation

Mutations D10A and H840A on WT cas9. Synonymous m…

Promoter

Pcon, PpspA-R3, PrhaB, and Ptet

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLY305

Plasmid

#184159

Purpose

Reporter circuit for hijacking mRNA of zraP as CRISPR RNA

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Inserts

dCas9
tetR
pspFΔHTH::λN22plus
mutsfgfp
rhaS

Use

Synthetic Biology

Tags

ASV tag and λN22plus

Expression

Bacterial

Mutation

K140N, Mutations D10A and H840A on WT cas9. Synon…

Promoter

Pcon, PpspA-zraP-22, PrhaB, and Ptet

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLY315

Plasmid

#184160

Purpose

Reporter circuit for hijacking mRNA of zntA as CRISPR RNA

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Inserts

dCas9
tetR
pspFΔHTH::λN22plus
sfgfp
rhaS

Use

Synthetic Biology

Tags

ASV tag and λN22plus

Expression

Bacterial

Mutation

Mutations D10A and H840A on WT cas9. Synonymous m…

Promoter

Pcon, PpspA-zntA-1034, PrhaB, and Ptet

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pLY162

Plasmid

#184138

Purpose

Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 1)

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Inserts

dCas9
tetR
pspFΔHTH::λN22plus
sfgfp
rhaS

Use

Synthetic Biology

Tags

ASV tag and λN22plus

Expression

Bacterial

Mutation

Mutations D10A and H840A on WT cas9. Synonymous m…

Promoter

Pcon, PpspA-R1, PrhaB, and Ptet

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLY163

Plasmid

#184141

Purpose

Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 2)

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Inserts

dCas9
tetR
pspFΔHTH::λN22plus
sfgfp
rhaS

Use

Synthetic Biology

Tags

ASV tag and λN22plus

Expression

Bacterial

Mutation

Mutations D10A and H840A on WT cas9. Synonymous m…

Promoter

Pcon, PpspA-R2, PrhaB, and Ptet

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLY168

Plasmid

#184142

Purpose

Reporter circuit for hijacking mRNA of arsenic responsive gene cluster as CRISPR RNA (site 2)

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Inserts

dCas9
tetR
pspFΔHTH::λN22plus
sfgfp
rhaS

Use

Synthetic Biology

Tags

ASV tag and λN22plus

Expression

Bacterial

Mutation

Mutations D10A and H840A on WT cas9. Synonymous m…

Promoter

Pcon, PpspA-Ar2, PrhaB, and Ptet

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLY300

Plasmid

#184158

Purpose

Reporter circuit for hijacking small RNA oxyS as CRISPR RNA

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Inserts

dCas9
tetR
pspFΔHTH::λN22plus
sfgfp
rhaS

Use

Synthetic Biology

Tags

ASV tag and λN22plus

Expression

Bacterial

Mutation

Mutations D10A and H840A on WT cas9. Synonymous m…

Promoter

Pcon, PpspA-oxyS-86, PrhaB, and Ptet

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLY320

Plasmid

#184161

Purpose

Reporter circuit for hijacking mRNA of cusCFAB as CRISPR RNA

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Inserts

dCas9
tetR
pspFΔHTH::λN22plus
sfgfp
rhaS

Use

Synthetic Biology

Tags

ASV tag and λN22plus

Expression

Bacterial

Mutation

Mutations D10A and H840A on WT cas9. Synonymous m…

Promoter

Pcon, PpspA-cusCFAB-656, PrhaB, and Ptet

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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BUN21/pML300 (recipient strain)

Bacterial Strain

#11675

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Bacterial Resistance

Spectinomycin

Species

Bacteriophage lambda

Available since

March 1, 2007

Availability

Academic Institutions and Nonprofits only
EcoFlex MoClo Extension, Volume I

Plasmid Kit

#1000000253

Purpose

Use the EcoFlex extension kit (volume one) to quickly and efficiently build a wide variety of gene expression constructs using the EcoFlex-standard MoClo system.

Depositor

Mikhail Shapiro

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only