We narrowed to 9,215 results for: Pol

1. pOpen-Pwopol

   Plasmid

   #165562

   Purpose

   DNA polymerase; additionally exhibits 3' to 5' exonuclease activity.

   Depositor

   Jennifer Molloy, Drew Endy, FreeGenes Project

   Insert

   Pwo DNA Polymerase
   

   Use

   Synthetic Biology

   Available Since

   Aug. 17, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. POLG-pcDNA3.1

   Plasmid

   #191487

   Purpose

   Expresses human POLG gene on pcDNA3.1 backbone. POLG gene is cloned with BamHI and XbaI restriction sites.

   Depositor

   Hans Zempel

   Insert

   POLG (POLG Human)
   

   Tags

   V5 tag, 6X His

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. EP-Pol vector

   Plasmid

   #131686

   Purpose

   A lentiviral vector that constitutively expresses an error-prone variant of the adenovirus DNA polymerase

   Depositor

   Matthew D. Shoulders

   Article

   Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

   Insert

   Adenovirus DNA polymerase
   

   Use

   Lentiviral

   Tags

   HA

   Mutation

   F421Y, D827A

   Available Since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOpen-K12polLF

   Plasmid

   #165511

   Purpose

   DNA pol fragment that retains 5'-3' polymerase activity and 3'-5' exonuclease activity but loses 5'-3' exonuclease activity.

   Depositor

   Jennifer Molloy, Drew Endy, FreeGenes Project

   Insert

   DNA Polymerase I, Large (Klenow) Fragment
   

   Use

   Synthetic Biology

   Available Since

   July 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pOpen-BsupolLF

   Plasmid

   #165563

   Purpose

   Bsu DNA Polymerase I, Large Fragment retains the 5'-3' polymerase activity of the Bacillus subtilis DNA polymerase I, but lacks the 5'-3' exonuclease domain. This large fragment naturally lacks 3'-5' exonuclease activity. Applications include random primer labeling, second strand cDNA synthesis, single dA tailing, and strand displacement DNA synthesis.

   Depositor

   Jennifer Molloy, Drew Endy, FreeGenes Project

   Insert

   Bsu DNA Polymerase I, Large Fragment
   

   Use

   Synthetic Biology

   Available Since

   Aug. 17, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pOpen-THEAQpolA

   Plasmid

   #165512

   Purpose

   DNA polymerase used in routine PCR method because of its high thermostability, standard testing, screening and output testing.

   Depositor

   Jennifer Molloy, Drew Endy, FreeGenes Project

   Insert

   Taq DNA Polymerase
   

   Use

   Synthetic Biology

   Available Since

   Aug. 9, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. FLAG-Pol-II 1-22

   Plasmid

   #35178

   Depositor

   Benjamin Blencowe

   Article

   Rosonina et al RNA. 2004 Apr;10(4):581-9.

   Insert

   RPBI (POLR2A Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Deletion of C-terminal domain repeats 23-52

   Promoter

   CMV

   Available Since

   March 30, 2012

   Availability

   Academic Institutions and Nonprofits only

8. FLAG-Pol-II 31-52

   Plasmid

   #35179

   Depositor

   Benjamin Blencowe

   Article

   Rosonina et al RNA. 2004 Apr;10(4):581-9.

   Insert

   RPBI (POLR2A Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Deletion of C-terminal domain repeats 1-30

   Promoter

   CMV

   Available Since

   Oct. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pOpen-9N7polA

   Plasmid

   #165500

   Purpose

   Thermophilic DNA polymerase; genetically engineered to have a decreased 3'-5' proofreading exonuclease activity

   Depositor

   Jennifer Molloy, Drew Endy, FreeGenes Project

   Insert

   9 degrees N-7 DNA polymerase
   

   Use

   Synthetic Biology

   Available Since

   July 26, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pOpen-BstpolLF

    Plasmid

    #165553

    Purpose

    Fragment retains 5'-3' polymerase activity from full length Bst DNA Polymerase, while lacking 5'-3' exonuclease activity. Suitable for applications requiring thermophilic strand displacement.

    Depositor

    Jennifer Molloy, Drew Endy, FreeGenes Project

    Insert

    Bst DNA Polymerase, Large Fragment
    

    Use

    Synthetic Biology

    Available Since

    Aug. 16, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pOpen-Bstpol

    Plasmid

    #165543

    Purpose

    Full length DNA polymerase from Bacillus stearothermophilus.

    Depositor

    Jennifer Molloy, Drew Endy, FreeGenes Project

    Insert

    Bst DNA Polymerase, Full Length
    

    Use

    Synthetic Biology

    Available Since

    Aug. 9, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pOpen-TthpolA

    Plasmid

    #165533

    Purpose

    DNA Polymerase whose activity is resistant to prolonged incubations at high temperatures (95 degrees C).

    Depositor

    Jennifer Molloy, Drew Endy, FreeGenes Project

    Insert

    Tth DNA polymerase
    

    Use

    Synthetic Biology

    Available Since

    Aug. 5, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pOpen-9N7polA (CT)

    Plasmid

    #165502

    Purpose

    9 Degrees North DNA polymerase variant with enhanced ability to incorporate modified substrates

    Depositor

    Jennifer Molloy, Drew Endy, FreeGenes Project

    Insert

    9 degrees N-7 DNA Polymerase chain terminating
    

    Use

    Synthetic Biology

    Available Since

    July 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. AE-Polyubiquitin-GCaMP6s

    Plasmid

    #106868

    Purpose

    Expresses polyubiquitin GCaMP6s, a genetically encoded calcium indicator, for live calcium imaging in Aedes aegypti mosquitoes.

    Depositor

    Omar Akbari

    Article

    Bui et al BMC Neurosci. 2019 Jun 17;20(1):27. doi:

    Insert

    AE-Polyubiquitin-GCAaMP6s
    

    Expression

    Insect

    Promoter

    Ae. aegypti polyubiquitin (PUb)

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. T. aq RNA Polymerase

    Plasmid

    #215349

    Purpose

    Express T. aq RNA Polymerase subunits in E. coli cells

    Depositor

    Julius Lucks

    Article

    Hertz et al Nucleic Acids Res. 2024 May 8;52(8):4466

    Insert

    RNA polymerase
    

    Tags

    6x His

    Expression

    Bacterial

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPolII-Luc

    Plasmid

    #17223

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

    Insert

    PolII promoter
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

17. pLKO.1P POLE1_1

    Plasmid

    #160762

    Purpose

    Suppress POLE1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLE1_1
    

    Use

    Lentiviral

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOpen-ECOpolA

    Plasmid

    #165532

    Purpose

    DNA polymerase that also exhibits 3'-5' and 5'-3' exonuclease activity.

    Depositor

    Jennifer Molloy, Drew Endy, FreeGenes Project

    Insert

    E. coli DNA Polymerase I
    

    Use

    Synthetic Biology

    Available Since

    Aug. 5, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pTol2CG NBT:NRGIIIa:polyA

    Plasmid

    #193012

    Purpose

    Tol2 transgenic construct that will express human Neuregulin1 Type IIIa in neurons

    Depositor

    William Talbot

    Article

    Lysko et al Hum Mutat. 2022 Sep;43(9):1216-1223. doi

    Inserts

    NBT
    NRG1 type IIIa (NRG1 Human)
    

    Use

    Transposon transgenesis

    Expression

    Mammalian

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. POLArIS_NbVim-cp10GFP

    Plasmid

    #171020

    Purpose

    Expresses NbVim-cp10GFP#2 in mammalian cells

    Depositor

    Sumio Terada

    Article

    Nakai et al Biochem Biophys Res Commun. 2021 Jun 2;5

    Insert

    anti-vimentin-nanobody VB6 (NbVim) tagged with circularly permutated superfolderGFP(cp10GFP)
    

    Tags

    cp10GFP

    Expression

    Bacterial and Mammalian

    Promoter

    CMV promoter

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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