We narrowed to 8,918 results for: Pol

1. pCas9-polyA

   Plasmid

   #72602

   Purpose

   express hCas9 byT7 promoter with polyadenine tails

   Depositor

   Tomoji Mashimo

   Article

   Yoshimi et al Nat Commun. 2016 Jan 20;7:10431. doi: 10

   Insert

   Cas9-polyadenine
   

   Use

   Tags

   81-bp polyadenylation

   Expression

   Mutation

   Promoter

   T7

   Available since

   July 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pOpen-BstpolLF

   Plasmid

   #165553

   Purpose

   Fragment retains 5'-3' polymerase activity from full length Bst DNA Polymerase, while lacking 5'-3' exonuclease activity. Suitable for applications requiring thermophilic strand displacement.

   Depositor

   Drew Endy, FreeGenes Project, Jennifer Molloy

   Insert

   Bst DNA Polymerase, Large Fragment
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 16, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. T. aq RNA Polymerase

   Plasmid

   #215349

   Purpose

   Express T. aq RNA Polymerase subunits in E. coli cells

   Depositor

   Julius Lucks

   Article

   Hertz et al Nucleic Acids Res. 2024 May 8;52(8):4466

   Insert

   RNA polymerase
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pOpen-Pwopol

   Plasmid

   #165562

   Purpose

   DNA polymerase; additionally exhibits 3' to 5' exonuclease activity.

   Depositor

   Drew Endy, FreeGenes Project, Jennifer Molloy

   Insert

   Pwo DNA Polymerase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 17, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pOpen-Bstpol

   Plasmid

   #165543

   Purpose

   Full length DNA polymerase from Bacillus stearothermophilus.

   Depositor

   Drew Endy, FreeGenes Project, Jennifer Molloy

   Insert

   Bst DNA Polymerase, Full Length
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 9, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pOpen-TthpolA

   Plasmid

   #165533

   Purpose

   DNA Polymerase whose activity is resistant to prolonged incubations at high temperatures (95 degrees C).

   Depositor

   Drew Endy, FreeGenes Project, Jennifer Molloy

   Insert

   Tth DNA polymerase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 5, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pOpen-K12polLF

   Plasmid

   #165511

   Purpose

   DNA pol fragment that retains 5'-3' polymerase activity and 3'-5' exonuclease activity but loses 5'-3' exonuclease activity.

   Depositor

   Drew Endy, FreeGenes Project, Jennifer Molloy

   Insert

   DNA Polymerase I, Large (Klenow) Fragment
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pOpen-9N7polA (CT)

   Plasmid

   #165502

   Purpose

   9 Degrees North DNA polymerase variant with enhanced ability to incorporate modified substrates

   Depositor

   Drew Endy, FreeGenes Project, Jennifer Molloy

   Insert

   9 degrees N-7 DNA Polymerase chain terminating
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. AE-Polyubiquitin-GCaMP6s

   Plasmid

   #106868

   Purpose

   Expresses polyubiquitin GCaMP6s, a genetically encoded calcium indicator, for live calcium imaging in Aedes aegypti mosquitoes.

   Depositor

   Omar Akbari

   Article

   Bui et al BMC Neurosci. 2019 Jun 17;20(1):27. doi:

   Insert

   AE-Polyubiquitin-GCAaMP6s
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Ae. aegypti polyubiquitin (PUb)

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pOpen-BsupolLF

    Plasmid

    #165563

    Purpose

    Bsu DNA Polymerase I, Large Fragment retains the 5'-3' polymerase activity of the Bacillus subtilis DNA polymerase I, but lacks the 5'-3' exonuclease domain. This large fragment naturally lacks 3'-5' exonuclease activity. Applications include random primer labeling, second strand cDNA synthesis, single dA tailing, and strand displacement DNA synthesis.

    Depositor

    Drew Endy, FreeGenes Project, Jennifer Molloy

    Insert

    Bsu DNA Polymerase I, Large Fragment
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 17, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pTol2CG NBT:NRGIIIa:polyA

    Plasmid

    #193012

    Purpose

    Tol2 transgenic construct that will express human Neuregulin1 Type IIIa in neurons

    Depositor

    William Talbot

    Article

    Lysko et al Hum Mutat. 2022 Sep;43(9):1216-1223. doi

    Inserts

    NBT
    NRG1 type IIIa (NRG1 Human)
    

    Use

    Transposon transgenesis

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. HDR-HaloPOLQ

    Plasmid

    #211637

    Purpose

    halo donor

    Depositor

    Agnel Sfeir

    Article

    Brambati et al Science. 2023 Aug 11;381(6658):653-660.

    Insert

    Puro-LoxHaloTag
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLKO.1P POLE1_1

    Plasmid

    #160762

    Purpose

    Suppress POLE1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLE1_1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPolII-Luc

    Plasmid

    #17223

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

    Insert

    PolII promoter
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pOpen-ECOpolA

    Plasmid

    #165532

    Purpose

    DNA polymerase that also exhibits 3'-5' and 5'-3' exonuclease activity.

    Depositor

    Drew Endy, FreeGenes Project, Jennifer Molloy

    Insert

    E. coli DNA Polymerase I
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. POLArIS_NbVim-cp10GFP

    Plasmid

    #171020

    Purpose

    Expresses NbVim-cp10GFP#2 in mammalian cells

    Depositor

    Sumio Terada

    Article

    Nakai et al Biochem Biophys Res Commun. 2021 Jun 2;5

    Insert

    anti-vimentin-nanobody VB6 (NbVim) tagged with circularly permutated superfolderGFP(cp10GFP)
    

    Use

    Tags

    cp10GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCellFree_G03 POLD1

    Plasmid

    #67128

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    POLD1 (POLD1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pLKO.1P POLD1_2

    Plasmid

    #160792

    Purpose

    Suppress POLD1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLD1_2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FRB-polySH35

    Plasmid

    #175234

    Purpose

    Bacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycin

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    NCK1 (NCK1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO.1P POLE1_2

    Plasmid

    #160764

    Purpose

    Suppress POLE1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLE1_2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge