We narrowed to 47,440 results for: REP

1. Cre Reporter

   Plasmid

   #62732

   Purpose

   Lentiviral Cre recombinase reporter; EF1a-loxP-DsRedExpress2-loxP-EGFP. Cre excises the loxP-flanked RFP-STOP cassette and switches fluorescence from red (RFP) to green (GFP).

   Depositor

   Niels Geijsen

   Article

   D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

   Insert

   LoxP-DsRED-STOP-LoxP-eGFP
   

   Use

   Cre/Lox and Lentiviral

   Tags

   LoxP sites

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-Alpha

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET29b-H6_Streptavidin_sfGFP

   Plasmid

   #124296

   Purpose

   His6-tagged Streptavidin GFP fusion protein

   Depositor

   Mark Arbing

   Insert

   His6 tag - TEV protease site - T7 tag - Streptavidin (15-159) - superfolder GFP
   

   Use

   Tags

   His6 and T7 tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. p21-Reporter

   Plasmid

   #52432

   Purpose

   DNA damage inducible Red Fluorescent Protein for mammalian expression

   Depositor

   Joel Voldman

   Article

   Fendyur et al Anal Chem. 2014 Aug 5;86(15):7598-605. d

   Insert

   p21-p53 Tandem Response Element Promoter
   

   Use

   Tags

   TurboRFP

   Expression

   Mammalian

   Mutation

   Promoter

   13X repeated p21-p53 response element

   Available since

   Oct. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. HA-KIF5B*-strep

   Plasmid

   #120170

   Purpose

   Expresses a chimera of HA tag, a KIF5B-based anterograde motor and streptavidin

   Depositor

   Juan Bonifacino

   Article

   Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

   Insert

   kinesin family member 5B (Kif5b Mouse)
   

   Use

   Tags

   HA and Streptavidin

   Expression

   Mammalian

   Mutation

   Truncated KIF5B: 1-555

   Promoter

   CMV

   Available since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Strep-KIFC1*-HA

   Plasmid

   #120171

   Purpose

   Expresses a chimera of streptavidin, a KIFC1-based retrograde motor and HA tag

   Depositor

   Juan Bonifacino

   Article

   Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

   Insert

   kinesin family member C1 (KIFC1 Human)
   

   Use

   Tags

   HA and Streptavidin

   Expression

   Mammalian

   Mutation

   Truncated KIFC1: 125-673 aa

   Promoter

   CMV

   Available since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. TMC1-TwinStrepII

   Plasmid

   #83267

   Purpose

   Expression of tagged protein in mammalian cells

   Depositor

   Peter Barr-Gillespie

   Insert

   TMC1 (Tmc1 Mouse)
   

   Use

   Tags

   Twin Strep Tag II

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. 2C::tdTomato Reporter

   Plasmid

   #40281

   Depositor

   Samuel Pfaff

   Article

   Macfarlan et al Nature. 2012 Jul 5;487(7405):57-63.

   Insert

   2C Promoter (2-cell stage promoter)
   

   Use

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 26, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSinREP5-iAchSnFR

   Plasmid

   #140643

   Purpose

   Acetylcholine sensor for sindbis virus

   Depositor

   J. Julius Zhu

   Article

   Borden et al bioRxiv 2020

   Insert

   Ach sensor V9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SP6

   Available since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cherry-LacRep

   Plasmid

   #18985

   Depositor

   Mirek Dundr

   Article

   Dundr et al J Cell Biol. 2007 Dec 17. 179(6):1095-10

   Insert

   LacI
   

   Use

   Tags

   AviTag, SV40 NLS, and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. miReporter-CAG

    Plasmid

    #82478

    Purpose

    microRNA reporter relying on a bidirectional CAG-based promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi

    Insert

    H2B-mCherry and H2B-Citrine. HygroR
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTD-NTwinStrep_Sm

    Plasmid

    #45937

    Purpose

    use to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Type

    Empty backbone

    Use

    Tags

    6xHis and Twin-Strep

    Expression

    Bacterial

    Mutation

    Promoter

    Trc (lactose/IPTG inducible)

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. miReporter-PGK

    Plasmid

    #82477

    Purpose

    microRNA reporter relying on a bidirectional PGK promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi

    Insert

    H2B-mCherry and H2B-Citrine. HygroR
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pREP-NFAT2

    Plasmid

    #11788

    Depositor

    Anjana Rao

    Insert

    NFAT2 (NFATC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. PM PKA Reporter

    Plasmid

    #104551

    Purpose

    Expresses Lyn-mTurquoise2-VASP at the plasma membrane of mammalian cells

    Depositor

    David Lawrence

    Article

    O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

    Insert

    Lyn-mTurquoise2-VASP 148 - 164
    

    Use

    Tags

    mTurquoise 2, HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1 alpha

    Available since

    March 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA4.TO-K5-2xCSTREP

    Plasmid

    #136154

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K5

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    K5
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTD-NTwinStrep_Km

    Plasmid

    #45938

    Purpose

    use to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Type

    Empty backbone

    Use

    Tags

    6xHis and Twin-Strep

    Expression

    Bacterial

    Mutation

    Promoter

    Trc (lactose/IPTG inducible)

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTD-CTwinStrep

    Plasmid

    #45939

    Purpose

    use to create C-terminal Twin-Strep-tag fusion proteins

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Type

    Empty backbone

    Use

    Tags

    Twin-Strep

    Expression

    Bacterial

    Mutation

    Promoter

    Trc (lactose/IPTG inducible)

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTD-NStrepHis

    Plasmid

    #45936

    Purpose

    use to create N-terminal Strep-tagII fusion proteins or dual tagged fusion with N-terminal Strep-tagII and C-terminal 6x His-tag

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Type

    Empty backbone

    Use

    Tags

    6xHis and Strep II

    Expression

    Bacterial

    Mutation

    Promoter

    Trc (lactose/IPTG inducible)

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX6P1-N-Strep2

    Plasmid

    #119758

    Purpose

    Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal Strep-II tag.

    Depositor

    Andrew Jackson, Martin Reijns

    Type

    Empty backbone

    Use

    Tags

    GST and Strep-tag II fusion tag

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. Nanog5P reporter

    Plasmid

    #16337

    Depositor

    Austin Cooney

    Article

    Gu et al Mol Cell Biol. 2005 Oct . 25(19):8507-19

    Insert

    Nanog promoter (Nanog Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Mouse Nanog promoter

    Available since

    Dec. 6, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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