We narrowed to 576 results for: ena/1000

1. pBC2T-actin

   Plasmid

   #108968

   Purpose

   expresses BC2-tag-actin in mammalian cells

   Depositor

   Ulrich Rothbauer

   Article

   Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

   Insert

   beta-actin (ACTB Human)
   

   Use

   Tags

   BC2-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcs2-Δhel1/Δhel2HRI-GFP-IRES-mCherry

   Plasmid

   #226101

   Purpose

   HRI stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells

   Depositor

   Michael Rape

   Article

   Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

   Insert

   HRI (EIF2AK1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   SIFI degron helices 1 & 2 deleted (Δ61-112)

   Promoter

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcs2-N-HRI(1-138)-GFP-IRES-mCherry

   Plasmid

   #226100

   Purpose

   HRI N-terminal region (residues 1-138) stability reporter construct for transient expression in mammalian cells

   Depositor

   Michael Rape

   Article

   Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

   Insert

   HRI (EIF2AK1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains only N-HRI (aa 1-138)

   Promoter

   Available since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. LSB-hsa-miR-675-3p

   Plasmid

   #103720

   Purpose

   Used to sense miRNA activity using fluorescence based tools. hsa-miR-675-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

   Depositor

   Ron Weiss

   Article

   Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

   Insert

   hsa-miR-675-3p target (MIR675 Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. LSB-hsa-miR-675-5p

   Plasmid

   #103721

   Purpose

   Used to sense miRNA activity using fluorescence based tools. hsa-miR-675-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

   Depositor

   Ron Weiss

   Article

   Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

   Insert

   hsa-miR-675-5p target (MIR675 Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMS22H-IRE

   Plasmid

   #133905

   Purpose

   Positive control when used in combination with pAWH-IRP. Expression of MS2BS-IRE site hybrid. Homology regions for recombination with pAWH

   Depositor

   Sebastian Maurer

   Article

   Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

   Insert

   IRE (Ftl1 Rat)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Pol III

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLJM1-FLAG-GFP-OSBP

   Plasmid

   #134659

   Purpose

   Flag and GFP-tagged OSBP

   Depositor

   Roberto Zoncu

   Article

   Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

   Insert

   OSBP (OSBP Human)
   

   Use

   Lentiviral

   Tags

   FLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRC54-DHX37-GFP

   Plasmid

   #192149

   Purpose

   express DHX37-GFP

   Depositor

   Sidi Chen

   Article

   Dong et al Cell. 2019 Aug 22;178(5):1189-1204.e23.

   Insert

   DHX37-GFP (Dhx37 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available since

   Nov. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. sg_Trp53_i4-ipUSEPR-TR657

   Plasmid

   #228930

   Purpose

   Knockdown of Trp53 in mammalian cells

   Depositor

   Sika Zheng

   Article

   Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

   Insert

   sg_Trp53_i4 (Trp53 sequence: GTCGCTACCTACAGCCAGGA, Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Integrin Beta1-mPA-GFP

    Plasmid

    #186302

    Purpose

    Expresses mPA-GFP tagged Integrin beta-1 in mammalian cells on a CMV promoter

    Depositor

    Pakorn Kanchanawong

    Article

    Jain et al Nat Protoc. 2022 Jul 27. pii: 10.1038/s4

    Insert

    Integrin Beta 1 (ITGB1 Human)
    

    Use

    Tags

    mPA-GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBKWH-p53

    Plasmid

    #133900

    Purpose

    Positive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWH

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Insert

    p53 (Trp53 Mouse)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    T7

    Available since

    June 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. H513 px330.sgActin.UTR.2

    Plasmid

    #138176

    Purpose

    CRISPR SONIC: Encodes codon-optimized SpCas9 and gRNA for targeting 3' UTR of β-actin.

    Depositor

    Wen Xue

    Article

    Mou et al Genome Med. 2019 Apr 16;11(1):21. doi: 1

    Insert

    sgActin (Actb Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRC55-GFP-DHX37

    Plasmid

    #192150

    Purpose

    express GFP-DHX37

    Depositor

    Sidi Chen

    Article

    Dong et al Cell. 2019 Aug 22;178(5):1189-1204.e23.

    Insert

    GFP-DHX37 (Dhx37 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBSWHhc-p53

    Plasmid

    #133903

    Purpose

    Positive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWH

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Insert

    p53 (TP53 Mouse)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    T7

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mature ISG15 C-term. domain (79-157)

    Plasmid

    #110764

    Purpose

    Bacterial expression of proISG15 C-term. domain

    Depositor

    David Komander

    Article

    Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

    Insert

    ISG15 (ISG15 Human)
    

    Use

    Tags

    N-His6-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. proISG15 C-term. domain (79-165)

    Plasmid

    #110763

    Purpose

    Bacterial expression of proISG15 C-term. domain

    Depositor

    David Komander

    Article

    Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

    Insert

    ISG15 (ISG15 Human)
    

    Use

    Tags

    N-His6-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SS-Cyclin D3

    Plasmid

    #215116

    Purpose

    Expresses SS-Cyclin D3 in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CCND3 (CCND3 Human)
    

    Use

    Tags

    SS (STREP-STREP)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX-DmrB-DmrB-Ripk3-2A-mCherry-puro

    Plasmid

    #231972

    Purpose

    Tet inducible expression of DmrB-Ripk3 with mCherry to induce Ripk3 necroptosis

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    Ripk3-deltaC dimerizing construct with bi-cistronic mCherry (Ripk3 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-P3-Alb-mScarlet

    Plasmid

    #183461

    Purpose

    To produce AAV with the P3 promoter to express Alb(mouse)-mScarlet fusion protein

    Depositor

    Hajime Hirase

    Article

    Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

    Insert

    Alb (Alb Mouse)
    

    Use

    AAV

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-P3-Alb-mNG

    Plasmid

    #183460

    Purpose

    To produce AAV with the P3 promoter to express Alb(mouse)-mNeonGreen fusion protein

    Depositor

    Hajime Hirase

    Article

    Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

    Insert

    Alb (Alb Mouse)
    

    Use

    AAV

    Tags

    mNeonGreen

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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