-
Plasmid#217014PurposeExpression of CDK2-7 chimera in E.coliDepositorUseTagsC3 protease cleavable GST tagExpressionBacterialMutationCDK2 (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N)PromoterAvailable sinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only
-
Nsp3 Mac1 - C2 (UCSF)
Plasmid#169209PurposeExpression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removalDepositorInsertSARS-CoV-2 Nsp3 Mac1
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceJuly 28, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
Nsp3 Mac1 - P43 (Oxford)
Plasmid#169210PurposeExpression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removalDepositorInsertSARS-CoV-2 Nsp3 Mac1
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceJuly 28, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST (AAV9)
Viral Prep#105448-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST (#105448). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST plasmid DNA. hSyn-driven expression of ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmRuby2Available sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
TBXTA-c005
Plasmid#139754PurposeFor protein expression/crystallography (PDB:6F58)DepositorInsertTBXT, DNA-binding domain, WT (TBXT Human)
UseTagsHis6ExpressionBacterialMutationContains only amino acids E41-S224PromoterT7Available sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
TBXTA-c006
Plasmid#139755PurposeFor protein expression/crystallography (PDB:6F59)DepositorInsertTBXT, DNA-binding domain, G177D (TBXT Human)
UseTagsHis6ExpressionBacterialMutationG177D; contains only amino acids E41-S224PromoterT7Available sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
RAB5AA-c006
Plasmid#175476PurposeProtein expression in bacterial cells. GTPase domain, G15-N184. Can be used for crystallography.DepositorInsertRAB5A (RAB5A Human)
UseTagsHis6-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
YSCD44A-c002
Plasmid#175493PurposeProtein expression in bacterial cells. Single chain Fv antibody to human CD44 Link domain. Can be used for crystallography.DepositorInsertSingle chain Fv CD44 antibody (H90)
UseTags3XFLAG-EK-His8 and pelBExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
TBXTA-c021
Plasmid#139758PurposeProtein expression/crystallography (PDB:5QT0)DepositorInsertTBXT, DNA-binding domain, G177D (TBXT Human)
UseTagsHis6-SUMOExpressionBacterialMutationG177D; contains only amino acids E41-N211PromoterT7Available sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
SDCBPA-c010
Plasmid#175482PurposeProtein expression in bacterial cells. PDZ1 and PDZ2 domains, A106-V288. Can be used for crystallography.DepositorInsertSDCBP (SDCBP Human)
UseTagsHis6-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
TBXTA-c020
Plasmid#139757PurposeFor protein expression/crystallography (PDB 5QS0)DepositorInsertTBXT, DNA-binding domain, WT (TBXT Human)
UseTagsHis6-SUMOExpressionBacterialMutationContains only amino acids E41-N211PromoterT7Available sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
TBXTA-c022
Plasmid#139759PurposeProtein expression/crystallographyDepositorInsertTBXT, DNA-binding domain, G177D (TBXT Human)
UseTagsHis6-SUMOExpressionBacterialMutationG177D; contains only amino acids E41-A214PromoterT7Available sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
Rab11A[8-175]-His-C
Plasmid#228040PurposeProtein purification construct. Rab11 crystallography truncation with a C-terminal cys for maleimide labeling.DepositorInsertRab11A[8-175]-His-C (RAB11A Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
CD44MMA-c002
Plasmid#175452PurposeProtein expression in bacterial cells (as inclusion bodies). CD44 Link module, Q23-D172. Used for crystallography.DepositorInsertCd44 (Cd44 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSuccessor
Plasmid#228391Purposeintended to be co-transformed along with pDawn-Ag43 to generate successor strain (SS-GFP) for multipattern biofilm lithographyDepositorInsertpConst23100-sfGFP__pLambda-DHFR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
Rab11A[8-175]-His-C Q70L
Plasmid#228042PurposeProtein purification construct. Rab11 crystallography truncation with a C-terminal cys for maleimide labeling. Constitutively active mutation (Q70L).DepositorInsertRab11A[8-175]-His-C Q70L (RAB11A Human)
UseTagsExpressionBacterialMutationQ70LPromoterAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
EPB41L3A-c021
Plasmid#175460PurposeProtein expression in bacterial cells. FERM domain, P107-L390. Used for crystallography (PDB:6IBE).DepositorInsertEPB41L3 (EPB41L3 Human)
UseTagsHis6-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDifferentiation
Plasmid#228393Purposeintended to be co-transformed along with pDawn-Ag43 to generate differentiation strain (DS-mRuby2) for multipattern biofilm lithographyDepositorInsertpLambda-luxR+LVAtag__pLux-mRuby2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBEL1584
Plasmid#139875PurposeExpression plasmid of rings 2 and 3 of LetB for crystallographyDepositorInsertLetB
UseTagsExpressionBacterialMutationLetB(159-383)-6xHisPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM3
Plasmid#154168PurposeEmpty vector with FLAG tag expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only