We narrowed to 10,799 results for: sacs

1. pscALPSpuro-HsACE2 (human)

   Plasmid

   #158081

   Purpose

   Expresses human ACE2 in mammalian cells

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   Human ACE2 (ACE2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pNHEJ-recAmu-sacB

   Plasmid

   #158717

   Purpose

   CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum and recAR61C allele.

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

   Insert

   recA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   R61C

   Promoter

   Available since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFS_0235-pET30-Fusicatenibacter_saccharivorans_ARRAY2_FaqI

   Plasmid

   #116954

   Purpose

   Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

   Depositor

   Randall Platt

   Article

   Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

   Insert

   Fusicatenibacter saccharivorans RT-Cas1-Cas2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLenti6.3-SaCas9-BlastR

   Plasmid

   #220966

   Purpose

   Expresses human codon-optimized SaCas9

   Depositor

   Hyongbum Kim

   Article

   Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

   Insert

   SaCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET-Sac-Aβ(M1-42/C18C33)

   Plasmid

   #173759

   Purpose

   Expresses the AβC18C33 peptide.

   Depositor

   James Nowick

   Article

   Zhang et al Biochemistry. 2022 Feb 15;61(4):252-264.

   Insert

   Aβ(M1-42/C18C33) (APP Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changed valine 18 to cysteine; changed glycine 33…

   Promoter

   T7

   Available since

   Aug. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pQCXIP mScarlet-ER [TM(SAC1)]

   Plasmid

   #186572

   Purpose

   Expression of the Endoplasmic Reticulum marker mScarlet-ER [TM(SAC1)] in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   SAC1 (SACM1L Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C-terminal transmembrane region of the protein: 5…

   Promoter

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDAI-SceI-SacB

   Plasmid

   #113635

   Purpose

   Broad host range replicative plasmid expressing the I-SceI homing endonuclease and the counterselectable marker SacB

   Depositor

   Miguel Valvano

   Article

   Aubert et al Methods Mol Biol. 2014;1197:311-27. doi:

   Insert

   sacB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Please see Depositor Comments

   Promoter

   sacB promoter

   Available since

   Nov. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TagBFP2-C1-SACM1LdeltaTMD-Fis1tail

   Plasmid

   #220078

   Purpose

   SAC1 with the TMD deleted and replaced with the Fis1tail to replace the TMD and target the mitochondria instead of the ER with a Blue Fluorophore

   Depositor

   Gerry Hammond

   Article

   Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

   Insert

   SACM1l-Fis1tail (SACM1L Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deltaTMD

   Promoter

   Available since

   May 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EGFP-1-SAC1deltaTMD-Fis1tail

   Plasmid

   #220077

   Purpose

   SAC1 with the TMD deleted and replaced with the Fis1tail to replace the TMD and target the mitochondria instead of the ER with a Green Fluorophore

   Depositor

   Gerry Hammond

   Article

   Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

   Insert

   SACM1l-Fis1tail (SACM1L Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deltaTMD

   Promoter

   Available since

   May 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBK1290-AAV-dSaCas9

    Plasmid

    #223139

    Purpose

    AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA Scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-sACE2v2.4(732)-8h

    Plasmid

    #154103

    Purpose

    Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2.4 (8his tag)

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Short GSG linker and 8his tag

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-G…

    Promoter

    CMV

    Available since

    June 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SAC1deltaTMD-FKBP-mCherry

    Plasmid

    #108123

    Purpose

    Recruitable cytosolic SAC1

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    SACM1L(1-520):FKBP1A(3-108):mCherry (SACM1L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SACM1L(1-520)

    Promoter

    CMV

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pscALPSpuro-SsACE2 (Pig)

    Plasmid

    #158085

    Purpose

    Expresses pig ACE2 in mammalian cells

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    Pig ACE2 (ACE2 Sus scrofa)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-sACE2v1-Fc(IgG1)

    Plasmid

    #145164

    Purpose

    Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity variant 1

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Fc region of human IgG1

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available since

    April 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mCherry-SAC1-FKBP

    Plasmid

    #108129

    Purpose

    Recruitable SAC1

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    mCherry:SACM1L:FKBP1A(3-108) (SACM1L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-sACE2v2-Fc(IgG1)

    Plasmid

    #145165

    Purpose

    Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 2

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Fc region of human IgG1

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-sACE2v2.4-8his

    Plasmid

    #149664

    Purpose

    Mammalian expression plasmid for his-tagged sACE2 high affinity variant 2.4

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Short GSG linker and 8his tag

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SAC1C389SdeltaTMD-FKBP-mCherry

    Plasmid

    #108124

    Purpose

    Recruitable cytosolic inactive SAC1

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    SACM1L(C389S; 1-520):FKBP1A(3-108):mCherry (SACM1L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SACM1L(C389S; 1-520)

    Promoter

    CMV

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-sACE2v2.4-sfGFP

    Plasmid

    #149666

    Purpose

    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 2.4

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Linker GSGGSGSGG and superfolder GFP

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti6.3-efSaCas9-BlastR

    Plasmid

    #220971

    Purpose

    Expresses human codon-optimized efSaCas9

    Depositor

    Hyongbum Kim

    Article

    Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

    Insert

    efSaCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge