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pAW-SpyCsn2

Plasmid

#113251

Purpose

E. coli expression vector for S. pyogenes Csn2-MBP-His

Depositor

Jennifer Doudna

Article

Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

Insert

S. pyogenes Csn2

Tags

MBP-6xHis

Expression

Bacterial

Promoter

T7

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pYOY153

Plasmid

#23107

Depositor

Linda Wordeman

Article

Andrews et al Dev Cell. 2004 Feb . 6(2):253-68.

Insert

Mitotic Centromere Associated Kinesin

Tags

GFP and His

Expression

Mammalian

Mutation

H530-A, R534-A, K537-A

Available Since

Feb. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pAW-SpyCas1

Plasmid

#113248

Purpose

E. coli expression vector for His-MBP-S. pyogenes Cas1

Depositor

Jennifer Doudna

Article

Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

Insert

S. pyogenes Cas1

Tags

6xHis-MBP

Expression

Bacterial

Promoter

T7

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAW-SpyCas2

Plasmid

#113249

Purpose

E. coli expression vector for His-MBP-S. pyogenes Cas2

Depositor

Jennifer Doudna

Article

Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

Insert

S. pyogenes Cas2

Tags

6xHis-MBP

Expression

Bacterial

Promoter

T7

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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GGDestX1-Amp

Plasmid

#157652

Purpose

Golden Gate assembly and cell-free (bacterial) expression of fusion-proteins

Depositor

Karmella Haynes

Article

Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

Type

Empty backbone

Use

Cell-free expression (e. coli sigma-70)

Expression

Bacterial

Promoter

OR2-OR1-Pr

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pDSAG

Plasmid

#62289

Purpose

docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, GFP marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Expression

Insect

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pDSARN

Plasmid

#62295

Purpose

docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, DsRed-NLS marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Expression

Insect

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pDSAT

Plasmid

#62290

Purpose

docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, mTurquoise CFP marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Expression

Insect

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pNOC-NLUC

Plasmid

#98141

Purpose

Gateway compatible construct for N' terminal fusion or promoter driven NanoLuciferase reporter, hygromycin resistance cassette

Depositor

Eva Farre

Article

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis

Tags

HA

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pattB-RfB2

Plasmid

#62296

Purpose

docking site transgenesis plasmid for Gateway Cloning and insect transgenesis

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Use

Unspecified

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pDSAYN

Plasmid

#62294

Purpose

docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, YFP-NLS marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Expression

Insect

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pRB326

Plasmid

#8667

Depositor

David Botstein

Article

Schatz et al Mol Cell Biol 1986 Nov;6(11):3722-33.

Insert

TUB1 (TUB1 Budding Yeast)

Use

Centromeric

Expression

Yeast

Available Since

June 30, 2005

Availability

Academic Institutions and Nonprofits only
pCAGGS mutated Pdx1 (DM#233)

Plasmid

#15072

Depositor

Douglas Melton

Article

Grapin-Botton et al Genes Dev. 2001 Feb 15. 15(4):444-54.

Insert

Pdx1

Expression

Mammalian

Mutation

DNA binding mutant

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pSN859

Plasmid

#190692

Purpose

Mammalian expression of kinesin scFv(H2) fused with Fc domain of chicken IgY

Depositor

Shinsuke Niwa

Article

Niwa et al Cytoskeleton (Hoboken). 2023 Apr 10. doi

Insert

kinesin scFv(H2)::Fc domain of chicken IgY

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pNOC-FLUC

Plasmid

#98140

Purpose

Gateway compatible construct for N' terminal fusion or promoter driven firefly luciferase reporter, hygromycin resistance cassette

Depositor

Eva Farre

Article

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis

Tags

HA

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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UB13p::dsSPL10 v01 (SPL10-RNAi)

Plasmid

#27409

Depositor

David Bartel

Article

Nodine et al Genes Dev. 2010 Dec 1. 24(23):2678-92.

Insert

SPL10 inverted (AT1G27370 Mustard Weed)

Available Since

Jan. 20, 2011

Availability

Academic Institutions and Nonprofits only

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pCre

Plasmid

#236212

Purpose

Arabinose-inducible Cre recombinase

Depositor

Matthew Waldor

Article

Basta et al Nat Microbiol. 2025 Mar 27. doi: 10.1038

Insert

Cre recombinase

Expression

Bacterial

Promoter

araBAD promoter

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pJH23

Plasmid

#159314

Purpose

Punc-25 GFP::SYD-2

Depositor

Mei Zhen

Article

Yeh et al J Neurosci. 2005 Apr 13;25(15):3833-41.

Insert

syd-2 (syd-2 Nematode)

Tags

GFP

Expression

Worm

Promoter

Punc-25

Available Since

Sept. 22, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pEF1a-IRES-Neo

Plasmid

#28019

Depositor

Thomas Zwaka

Article

Dejosez et al Genes Dev. 2010 Jul 15. 24(14):1479-84.

Type

Empty backbone

Expression

Mammalian

Available Since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

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luxB:cp157Venus+luxA/pRSETB Duet

Plasmid

#197583

Purpose

BRET lux luciferase with cp157Venus for bacterial expression

Depositor

Takeharu Nagai

Article

Kaku et al Sci Rep. 2021 Jul 22;11(1):14994. doi: 1

Insert

LuxA LuxBcp157Venus

Use

E.coli expression

Tags

His X 6

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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co luxE pcDNA3.1(+)

Plasmid

#129089

Purpose

Expression of luxE in mammalian cells

Depositor

Stefan Hell

Article

Gregor et al Proc Natl Acad Sci U S A. 2019 Dec 2. pi

Insert

co luxE

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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co luxC pcDNA3.1(+)

Plasmid

#129087

Purpose

Expression of luxC in mammalian cells

Depositor

Stefan Hell

Article

Gregor et al Proc Natl Acad Sci U S A. 2019 Dec 2. pi

Insert

co luxC

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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co luxD pcDNA3.1(+)

Plasmid

#129088

Purpose

Expression of luxD in mammalian cells

Depositor

Stefan Hell

Article

Gregor et al Proc Natl Acad Sci U S A. 2019 Dec 2. pi

Insert

co luxD

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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co frp pcDNA3.1(+)

Plasmid

#129090

Purpose

Expression of frp in mammalian cells

Depositor

Stefan Hell

Article

Gregor et al Proc Natl Acad Sci U S A. 2019 Dec 2. pi

Insert

co frp

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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co luxA pcDNA3.1(+)

Plasmid

#129085

Purpose

Expression of luxA in mammalian cells

Depositor

Stefan Hell

Article

Gregor et al Proc Natl Acad Sci U S A. 2019 Dec 2. pi

Insert

co luxA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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co luxB pcDNA3.1(+)

Plasmid

#129086

Purpose

Expression of luxB in mammalian cells

Depositor

Stefan Hell

Article

Gregor et al Proc Natl Acad Sci U S A. 2019 Dec 2. pi

Insert

co luxB

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pLV CMV-TERRA

Plasmid

#111375

Purpose

TERRA expression contains approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences

Depositor

Klaus Holzmann

Article

Kreilmeier et al Genes (Basel). 2016 Aug 16;7(8). pii: ge

Insert

TERRA polyA

Use

Lentiviral

Promoter

CMV

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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luxA+luxB/pRSETB Duet

Plasmid

#197581

Purpose

Expressing luxA and luxB under dual T7 promoter

Depositor

Takeharu Nagai

Article

Kaku et al Sci Rep. 2021 Jul 22;11(1):14994. doi: 1

Insert

LuxA LuxB

Tags

T7 tag and 6xHis

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCMVlux-cp157Venus

Plasmid

#197584

Purpose

BRET lux luciferase with cp157Venus for mammalian expression

Depositor

Takeharu Nagai

Article

Kaku et al Sci Rep. 2021 Jul 22;11(1):14994. doi: 1

Insert

human codon-optimized Lux-cp157Venus

Expression

Mammalian

Promoter

CMV promoter

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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luxB:Venus+luxA/pRSETB Duet

Plasmid

#197582

Purpose

BRET lux luciferase with Venus for bacterial expression

Depositor

Takeharu Nagai

Article

Kaku et al Sci Rep. 2021 Jul 22;11(1):14994. doi: 1

Insert

LuxA LuxBVenus

Use

E.coli expression

Tags

His X 6

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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