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Plasmid#69974PurposeExpresses FnCpf1 and spacers 1-4 of CRISPR array. Cas genes are removed.DepositorInsertFnCpf1 locus without Cas genes
UseCRISPRTagsExpressionBacterialMutationCas genes are deleted from locusPromoterAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
MLF2-GFP-3xFLAG
Plasmid#230986PurposeExpresses GFP tagged MLF2 in mammalian cellsDepositorInsertMLF2 (MLF2 Human)
UseTags3xFLAG and GFPExpressionMammalianMutationPromoterT7Available sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1alpha-soCoChR-tdTomato
Plasmid#107714PurposeA somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. EF1alpha promoterDepositorInsertsoCoChR-tdTomato
UseAAVTagsKA2 and tdTomatoExpressionMammalianMutationPromoterEF1alphaAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pY002 (pFnCpf1_min)
Plasmid#69975PurposeExpresses FnCpf1 and spacers 1-4 of CRISPR array under artificial promoter. Cas genes are removed.DepositorInsertFnCpf1 locus without Cas genes nd artificial promoter
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT/TO/FLAG/HA-DEST DICER
Plasmid#19881DepositorInsertDicer 1, ribonuclease type III (DICER1 Human)
UseTagsFLAG/HAExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin-FLEX-soCoChR-GFP
Plasmid#107712PurposeA somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. Cre-dependent virusDepositorHas ServiceAAV9InsertsoCoChR-GFP
UseAAVTagsEGFP and KA2ExpressionMammalianMutationPromoterhSynapsinAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin- soCoChR-GFP
Plasmid#107708PurposeA somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. Human Synapsin (hSynapsin) promoterDepositorHas ServiceAAV9InsertsoCoChR-GFP
UseAAVTagsEGFP and KA2ExpressionMammalianMutationPromoterhSynapsinAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
px458_2A_GFP_sgRNA_G3BP1_G1
Plasmid#127114DepositorInsertgRNA G3BP1 (G3BP1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFRT_TO_eGFP_CELF1
Plasmid#106100PurposeInducible gene expression of EGFP - CELF1DepositorInsertCELF1 (CELF1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNAD1
Plasmid#154347PurposepUC19 backbone with editing template for homologous recombination mediated deletion of Nitrate reductase gene of N. oceanica and selection based on zeocin resistanceDepositorInsertShBle
UseMicroalgaeTagsExpressionBacterialMutationnonePromoterViolaxanthin Chlorophyll a Binding Protein Precur…Available sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsEDC4
Plasmid#66597PurposeExpresses lambdaN-HA-tagged EDC4 in mammalian cellsDepositorInsertEDC4 (EDC4 Human)
UseTagslambdaN-HAExpressionMammalianMutationPromoterCMVAvailable sinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOP22-5'UTR-BBa_B0034
Plasmid#216626PurposeNanoLuc reporter plasmid to optimize chloroplast cell-free systems with RBS BBa_B0034 in 5'UTRDepositorInsertT7p-5'B0034-NanoLuc-3'TMV
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOP01-UTC-7.0
Plasmid#216625PurposeNanoLuc reporter plasmid to optimize chloroplast cell-free systems, universal test constructDepositorInsertT7p-5'g10-NanoLuc-3'TMV
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD/Paro-IFN
Plasmid#205439PurposeA donor vector plasmid for Cre-mediated integration of paromomycin resistance (Paro) and dog IFNα-4 (IFNα-4) genesDepositorInsertsdog interferon α-4 gene
paromomycin resistance gene
UseCre/Lox; Chlamydomonas expressionTagsExpressionMutationPromoterHeat shock protein 70A (HSP70A)/ribulose bisphosp…Available sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-soCoChR-GFP
Plasmid#107711PurposeA somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. CaMKII promoterDepositorInsertsoCoChR-GFP
UseAAVTagsEGFP and KA2ExpressionMammalianMutationPromoterCaMKIIAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-NTerm 6xHis-zmADH-T500
Plasmid#92228PurposeExpression of zmADH using the p70a promoter and UTR1DepositorInsertNTerm 6xHis Tagged zmADH
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationPromoterp70aAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR4-EGFP_opt
Plasmid#106334PurposeGateway Entry vector for EGFP (codon optimized for efficient gene synthesis)DepositorInsertEGFP
UseGateway entry vectorTagsExpressionBacterial and MammalianMutationcodon optimized for gene synthesis without affect…PromoterAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
px458_2A_GFP_sgRNA_ELAVL1
Plasmid#106106PurposeCRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting ELAVL1DepositorInsertgRNA targeting ELAVL1 (ELAVL1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFRT/FLAG/HA-DEST EIF2C1
Plasmid#19887DepositorInsertEukaryotic translation initiation factor 2C, 1 (AGO1 Human)
UseTagsFLAG/HAExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsDCP1a_G
Plasmid#146400PurposeMammalian Expression of HsDcp1aDepositorInsertHsDcp1a (DCP1A Human)
UseTagsExpressionMammalianMutationtwo silent mutations T237C and G1716T compared to…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only