We narrowed to 1,934 results for: IRES-GFP

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pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#3

Plasmid

#197426

Purpose

π-element knock-in into exon 5 of MAPRE1

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

MAPRE1 gRNA #3 (targets Exon 5) for π-element knock-in (MAPRE1 Human)

Expression

Mammalian

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2-Cep123-C2 domain

Plasmid

#73329

Purpose

Encodes the distal appendage targeting domain of isoform 1 of Cep123 (residues 500-600) fused to EGFP

Depositor

Michel Bornens

Article

Sillibourne et al Biol Open. 2013 Apr 9;2(6):535-45. doi:

Insert

Cep123 (CEP89 Human)

Tags

EGFP tag

Expression

Mammalian

Promoter

CMV

Available Since

April 4, 2016

Availability

Academic Institutions and Nonprofits only

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6K-loop-->R GLUT1-GFP

Plasmid

#200098

Purpose

human GLUT1 with K-->R mutation of 6 lysines in the major cytoplasmic loop and C-terminal GFP tag in pQCXIP vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

GLUT1 (SLC2A1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

K-->R mutations of amino acids 225, 229, 230, …

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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1K245 GLUT1-GFP

Plasmid

#200097

Purpose

human GLUT1 with a single cytosolic lysine (K245) and C-terminal GFP tag in pQCXIP vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

GLUT1 (SLC2A1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

K-->R mutations of amino acids 6, 7, 225, 229,…

Promoter

CMV

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#1

Plasmid

#197424

Purpose

π-element knock-in into exon 5 of MAPRE1

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

MAPRE1 gRNA #1 (targets Exon 5) for π-element knock-in (MAPRE1 Human)

Expression

Mammalian

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#2

Plasmid

#197425

Purpose

π-element knock-in into exon 5 of MAPRE1

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

MAPRE1 gRNA #2 (targets Exon 5) for π-element knock-in (MAPRE1 Human)

Expression

Mammalian

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe-GFP-IKBalpha-wt

Plasmid

#15263

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

IKB alpha (NFKBIA Human)

Use

Retroviral

Expression

Mammalian

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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EGFP-NIP45 D394R

Plasmid

#210265

Purpose

Inducible expression of N-terminally EGFP-tagged NIP45 D394R resistant to siRNA. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 D394R siRES (NFATC2IP Synthetic)

Tags

EGFP

Expression

Mammalian

Mutation

D394R, siRES

Promoter

CMV/TetO2

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-NIP45 delta-N1

Plasmid

#210268

Purpose

Inducible expression of N-terminally EGFP-tagged NIP45 delta-N1 resistant to siRNA. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 delta-N1 (aa 208-419) siRES (NFATC2IP Synthetic)

Tags

EGFP

Expression

Mammalian

Mutation

delta-N1, siRES

Promoter

CMV/TetO2

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-NIP45 delta-N2

Plasmid

#210269

Purpose

Inducible expression of N-terminally EGFP-tagged NIP45 delta-N2 resistant to siRNA. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 delta-N2 (aa 261-419) siRES (NFATC2IP Synthetic)

Tags

EGFP

Expression

Mammalian

Mutation

delta-N2, siRES

Promoter

CMV/TetO2

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19-π-EB1_EF1α-EGFP

Plasmid

#197430

Purpose

Homology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

EF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)

Use

CRISPR and Synthetic Biology

Tags

EGFP, GCN4 leucine zipper, LOV2, and Zdk1

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pRSET.GltI253-cpGFP.L1LV/L2NP

Plasmid

#41733

Purpose

a single-wavelength intracellular glutamate sensor constructed from E. coli Gltl and cpGFP. For bacterial cytoplasmic expression

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

Insert

GltI based Glutamate Biosensor

Use

Glutamate biosensor

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Bacterial

Mutation

cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…

Promoter

T7

Available Since

Jan. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pMCB306-GFP-mito

Plasmid

#229232

Purpose

Expresses GFP localized to mitochondria

Depositor

Suzanne Pfeffer

Article

Bagnoli et al Proc Natl Acad Sci U S A. 2025 Feb 4;122

Insert

EGFP-MAOA (MAOA Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMIH-TFAM-GFP

Plasmid

#113704

Purpose

Fluorescent fusion protein used to visualise mitochondrial nucleoids, with hygromycin selection

Depositor

Benjamin Kile

Article

McArthur et al Science. 2018 Feb 23;359(6378). pii: 359

Insert

TFAM (Tfam Mouse)

Use

Retroviral

Tags

eGFP

Expression

Mammalian

Promoter

MSCV

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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PB-PARP1-Apex2–eGFP

Plasmid

#211555

Purpose

A piggyBac vector containing CMV-PARP1-Apex2-eGFP-IRES-Neo cassette.

Depositor

Chris Lord

Article

Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

Insert

PARP1 (PARP1 Human)

Use

Piggybac

Tags

Apex2-eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-PARP1-E988K

Plasmid

#211580

Purpose

CMV driven PARP1-E988K -eGFP expressing construct with IRES-Neomycin selection

Depositor

Chris Lord

Article

Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

Insert

PARP1 (PARP1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

Contains E988K mutation

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1_c-SRC

Plasmid

#171850

Purpose

pEGFP-N1_c-SRC

Depositor

Michele Pagano

Article

Moro et al Nat Cell Biol. 2020 Sep;22(9):1130-1142.

Insert

c-SRC (SRC Human)

Tags

GFP

Expression

Mammalian

Mutation

Added a linker sequence in 3' of c-SRC: GSGS

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pmGFP-P2A-K0-P2A-RFP

Plasmid

#105686

Purpose

Double fluorescent stalling reporter K0

Depositor

Ramanujan Hegde

Article

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Inserts

P2A-3xFLAG-VHPbeta
K0
P2A-mCherry

Expression

Mammalian

Promoter

CMV (from vector)

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs-puro GFP-p62

Plasmid

#38277

Depositor

Noboru Mizushima

Article

Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

Insert

Sequestosome-1 (Sqstm1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pMXs-puro GFP-p62 dC

Plasmid

#38282

Depositor

Noboru Mizushima

Article

Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

Insert

Sequestosome-1 (Sqstm1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Truncate mutant 1-265 amino acids residues

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

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