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AAV2/9-CW3SL-mCherry-DrTrkB

Plasmid

#184002

Purpose

AAV production for expression of optically controlled eDrTrkB with N-terminal fusion of mCherry

Depositor

Dmitry Gerashchenko, Vladislav Verkhusha

Article

Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

Insert

eDrTrkB (NTRK2 Human)

Use

AAV

Tags

Ig kappa secretory signal, mCherry, Deinococcus r…

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pSC5GGv2

Plasmid

#188604

Purpose

pSC5 Golden-Gate Cloning Plasmid (BsaI - Site 2)

Depositor

Bogumil Karas

Article

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology; Diatom expression

Expression

Bacterial and Yeast

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-LacI-HIPK2

Plasmid

#179269

Purpose

Expresses GFP-LacI-HIPK2 fusion protein

Depositor

Lienhard Schmitz

Article

Haas et al Front Cell Dev Biol. 2020 Sep 1;8:852. d

Insert

homeodomain interacting protein kinase 2 (HIPK2 Human)

Tags

GFP-LacI

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pAGE2.0-i

Plasmid

#194639

Purpose

Empty pAGE2.0 plasmid with pBAD inducible promoter

Depositor

Bogumil Karas

Article

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology; Diatom expression

Expression

Bacterial and Yeast

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pBS mEya1 (CT#671)

Plasmid

#13971

Depositor

Cliff Tabin

Article

Heanue et al Mech Dev. 2002 Feb . 111(1-2):75-87.

Insert

Eya1 (Eya1 Mouse)

Expression

Bacterial

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pCR8/GW/TOPO-SRF

Plasmid

#98618

Purpose

Gateway entry TOPO TA cloning vector for SRF

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

SRF (Srf Mouse)

Use

Topo ta cloning vector

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-2B-Nkx2-5

Plasmid

#98612

Purpose

Gateway entry vector for NKX2-5

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

NKX2-5 (Nkx2-5 Mouse)

Use

Entry vector

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-lifeactin

Plasmid

#229607

Purpose

Expresses mNeonGreen-lifeactin fusion in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

Insert

mNeonGreen

Expression

Mammalian

Available Since

Oct. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pSB02

Plasmid

#230991

Purpose

B. dendrobatidis plasmid for integration of target sequence at URA3 locus, blasticidin S selection

Depositor

Lillian Fritz-Laylin

Article

Brody et al Proc Natl Acad Sci U S A. 2025 Nov 4;122

Insert

BdURA3_LHA2-Hsbsr-ScADH1ter

Use

Bd gene targeting vector

Tags

bsr

Expression

Bacterial

Available Since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCytERM-(n1)StayGold(E138D)(c4)-N1

Plasmid

#229596

Purpose

Expresses P450-(n1)StayGold(E138D)(c4) in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

Insert

(n1)StayGold(E138D)(c4)

Expression

Mammalian

Available Since

July 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCytERM-mStayGold(E138D)-N1

Plasmid

#229569

Purpose

Expresses P450-mStayGold(E138D) in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

Insert

mStayGold(E138D)

Expression

Mammalian

Available Since

July 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCytERM-StayGold-N1

Plasmid

#229568

Purpose

Expresses P450-StayGold fusion in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

Insert

StayGold

Expression

Mammalian

Available Since

July 16, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP-lifeactin

Plasmid

#229606

Purpose

Expresses EGFP-lifectin fusion in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

Insert

EGFP

Expression

Mammalian

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pSC5GGv1_ShBle

Plasmid

#188603

Purpose

pSC5GGv1 with Sh ble insert for yeast selection

Depositor

Bogumil Karas

Article

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology; Diatom expression

Expression

Bacterial and Yeast

Available Since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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M2C5

Plasmid

#188606

Purpose

Minimal Conjugative Plasmid 2

Depositor

Bogumil Karas

Article

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology; Diatom expression

Expression

Bacterial and Yeast

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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M1C1

Plasmid

#188609

Purpose

Minimal Conjugative Plasmid 1

Depositor

Bogumil Karas

Article

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology; Diatom expression

Expression

Bacterial and Yeast

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSF11-wmiRFP2

Plasmid

#178969

Purpose

Expresses miRFP2 in worm cells

Depositor

Kiryl Piatkevich

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

wmiRFP2

Tags

no

Expression

Worm

Mutation

NO

Promoter

tag-168 promoter

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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bdSUMO-5.3-GFP-HiBit

Plasmid

#171014

Purpose

For bacterial expression of bdSUMO tagged muGFP with 5.3 peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-5.3-muGFP-HiBiT

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i3-msfGFP

Plasmid

#180322

Purpose

mammalian expression of human SEPT9_i3 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v3 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mBaoJin-P2A-FusionRed

Plasmid

#229572

Purpose

Expresses mBaoJin and FusionRed in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

Insert

mBaoJin

Use

AAV

Expression

Mammalian

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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