We narrowed to 1,854 results for: rigi

1. punc-119cR

   Plasmid

   #21596

   Depositor

   Al Fisher

   Article

   Ferguson et al Plasmid. 2009 Sep;62(2):140-5. doi: 10.1

   Insert

   unc-119:mCherry (unc-119 Nematode)
   

   Use

   Adds unc-119 marker to amp resistant plasmids

   Tags

   mCherry

   Expression

   Worm

   Mutation

   N/A

   Promoter

   Available Since

   Aug. 26, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBTK1003 (pSL2)

   Plasmid

   #190998

   Purpose

   Expresses red chromoprotein on a broad host range RSF1010 origin plasmid

   Depositor

   Jeffrey Barrick

   Article

   Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

   Insert

   red chromoprotein
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   CP25

   Available Since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pconst #5-RFP

   Plasmid

   #81079

   Purpose

   constitutive promoter expressing RFP, biobrick construction

   Depositor

   Jay Keasling, Paul Adams

   Article

   Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

   Insert

   RFP
   

   Use

   P15a origin, constitutive promoter, kanr

   Tags

   Expression

   Mutation

   Promoter

   Constitutive Promoter - BBa_J23110

   Available Since

   Aug. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMRMTK-clo10

   Plasmid

   #203961

   Purpose

   Cloning plasmid containing the erythromycin resistance gene insert

   Depositor

   Nathan Crook

   Article

   van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

   Insert

   Erythromycin Resistance Gene
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   N/A

   Available Since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. CP1300-ABEmax

   Plasmid

   #119810

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

   Insert

   ecTadA-ecTadA*(7.10)-nSpCas9 (CP1300)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pconst #4-RFP

   Plasmid

   #81078

   Purpose

   constitutive promoter expressing RFP, biobrick construction

   Depositor

   Jay Keasling, Paul Adams

   Article

   Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

   Insert

   RFP
   

   Use

   P15a origin, constitutive promoter, kanr

   Tags

   Expression

   Mutation

   Promoter

   Constitutive Promoter - BBa_J23107

   Available Since

   Aug. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6-SacB-Cas9-T2A-mCherry_(BbsI)

   Plasmid

   #117070

   Purpose

   Encodes Cas9, T2A-mCherry, and AmpR which creates a CRISPR system application with reliable positive selection

   Depositor

   Kim Failor

   Inserts

   Cas9
   SacB
   

   Use

   CRISPR

   Tags

   3x FLAG, NLS, and T2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available Since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pconst #1-RFP

   Plasmid

   #81075

   Purpose

   constitutive promoter expressing RFP, biobrick construction

   Depositor

   Jay Keasling, Paul Adams

   Article

   Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

   Insert

   RFP
   

   Use

   P15a origin, constitutive promoter, kanr

   Tags

   Expression

   Mutation

   Promoter

   Constitutive Promoter - BBa_J23119

   Available Since

   Aug. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAL1

   Plasmid

   #197285

   Purpose

   Shuttle plasmid that replicates in E. coli, S. cerevisiae, and A. laidlawii. Contains the OriC from A. laidlawii 8195

   Depositor

   Bogumil Karas

   Article

   Nucifora et al ACS Synth Biol. 2024 Jan 19;13(1):45-53.

   Insert

   Origin of replication of A. laidlawii 8195: whole dnaA gene, plus upstream and downstream intergenic regions
   

   Use

   Synthetic Biology; Bacterial and yeast cloning

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. GTT-g

    Plasmid

    #81109

    Purpose

    Gene Tagging vector TRP/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)

    Depositor

    Serge Pelet

    Article

    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

    Insert

    sfGFP
    

    Use

    Cre/Lox

    Tags

    sfGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. GTH-r

    Plasmid

    #81106

    Purpose

    Gene Tagging vector HIS/RFP - Plasmid for monomeric Cherry (mCherry) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)

    Depositor

    Serge Pelet

    Article

    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

    Insert

    mCherry
    

    Use

    Cre/Lox

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPpT4- TEF2prom-TIR1F74G-FLAG

    Plasmid

    #189727

    Purpose

    Enables expression of OsTIR1(F74G) from the TEF2 promoter, used to implement the AID2 system in Komagataella phaffii (original name pLB219)

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

    Insert

    OsTIR1
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    TEF2

    Available Since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Nup54 no FG-mEGFP494(0)

    Plasmid

    #164269

    Purpose

    Mammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 5 amino acids at the carboxyl end of mEGFP

    Depositor

    Sanford Simon

    Article

    Pulupa et al Elife. 2020 Dec 21;9. pii: 60654. doi: 1

    Insert

    Nup54 (NUP54 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    deleted the FG region of Nup54

    Promoter

    CMV

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTH652-RLuc/maxFLuc

    Plasmid

    #29698

    Depositor

    Tobias von der Haar

    Article

    Chu et al Nucleic Acids Res. 2011 Aug;39(15):6705-

    Insert

    Firefly Luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    All codons of the original FLuc gene were exchang…

    Promoter

    Available Since

    Sept. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. BB3aZ_14*

    Plasmid

    #98542

    Purpose

    empty BB3 for insertion and overexpression of 1 gene in P. pastoris

    Depositor

    Diethard Mattanovich, Brigitte Gasser, Michael Sauer

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-CTE-Luc

    Plasmid

    #60881

    Purpose

    Reporter construct to study unspliced RNA export. Coding sequence of luciferase gene was sandwiched between 5' and 3' splice site sequences originating from the HIV gag/pol gene followed by CTE

    Depositor

    Vinay Nandicoori

    Article

    Rajanala et al PLoS One. 2012;7(1):e29921. doi: 10.1371

    Insert

    Luc-CTE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJUMP27ts[mCherry]-1A(lacZ)

    Plasmid

    #126980

    Purpose

    Level 1 vector. With lacZ as alternative cloning reporter. Thermosensitive origin pSC101 (low copy number). Constitutive mCherry in downstream secondary site.

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    RepA A56V thermosensitive mutation

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. THI VP64 pGAP T5

    Plasmid

    #161492

    Purpose

    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T5_gRNA_MS2

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (Targeting T5 in the THI11 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. THI VP64 pGAP T1

    Plasmid

    #161488

    Purpose

    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T1_gRNA_MS2

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (Targeting T1 in the THI11 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCK300

    Plasmid

    #87766

    Purpose

    empty control plasmid, three terminators to place inserts between for insulation, ampR, pBR322 origin, lacI

    Depositor

    John Heap

    Article

    Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    None

    Available Since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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