173,178 results
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Plasmid#73915Purposetissue-specific multiplex gRNA plasmidDepositorInsert:tRNA:gRNA:tRNA:gRNA:tRNA
UseCRISPRExpressionInsectPromoterUAS-DSCPAvailable SinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-CD33-COMP5AP-AviTag-9xHis
Plasmid#157258PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SIGLEC1(Trunc2)-Fc(DAPA)-AviTag-6xHis
Plasmid#156999PurposeMammalian expression of cell-surface protein partial extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertSIGLEC1 (SIGLEC1 Human)
TagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SIGLEC6-Fc(DAPA)-AviTag-6xHis
Plasmid#156762PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertSIGLEC6 (SIGLEC6 Human)
TagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-MAG-Fc(DAPA)-AviTag-6xHis
Plasmid#156882PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertMAG (MAG Human)
TagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available SinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-MAG-COMP5AP-AviTag-9xHis
Plasmid#157446PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-Flag-hDcp2
Plasmid#72213Purposemammalian expression of Flag-tagged human Dcp2DepositorInsertDcp2 (DCP2 Human)
TagsFlagExpressionMammalianMutationQ170R (please see depositor's comment below)PromoterCMVAvailable SinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-N-TEV-Twin-Strep
Plasmid#202526PurposeExpress C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cellDepositorTypeEmpty backboneTagsTEV cleavable site and Twin-strep-tagExpressionMammalianPromoterCAGAvailable SinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDule-tfmF A65V S158A
Plasmid#85484PurposeMachinery plasmid expressing permissive Mj tfmF synthetase and cognate amber suppressing tRNADepositorInserttri-fluoromethyl-phenylalanine Mj synthetase A65V S158A
TagsNoneExpressionBacterialMutationY32V F108W Q109M I159M with 65Val and 158AlaPromoterlpp (constitutive)Available SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
IL2 F42A pAcGP67a
Plasmid#41807DepositorInsertIL2 (IL2 Human)
Tags6x HisExpressionInsectMutationPhenylalanine 42 to AlaninePromoterPolyhedronAvailable SinceJan. 3, 2013AvailabilityAcademic Institutions and Nonprofits only