We narrowed to 14,482 results for: 112

1. pSN-A112C-CP8B1

   Plasmid

   #191240

   Purpose

   Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies.

   Depositor

   Alessandro Senes

   Article

   Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

   Insert

   nuc (nuclease A), CYP8B1 (CYP12) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
   

   Tags

   nuclease A from S. aureus fused to the poly-Leu T…

   Expression

   Bacterial

   Mutation

   Changed Alanine 112 to Cysteine in SN, and predic…

   Promoter

   T7 promoter

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pvLEA22mer_mutant_3_mCh-2xFKBP (pBS1123)

   Plasmid

   #185318

   Purpose

   For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_3 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   pvLEA22mer_mutant_3
   

   Expression

   Mammalian

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. PvLEA4_repeats_k5_1_mCh-2xFKBP (pBS1122)

   Plasmid

   #185317

   Purpose

   For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k5_1 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   PvLEA4_repeats_k5_1
   

   Expression

   Mammalian

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pvLEA22mer_mutant_10_mCh-2xFKBP (pBS1121)

   Plasmid

   #185316

   Purpose

   For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_10 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   pvLEA22mer_mutant_10
   

   Expression

   Mammalian

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT5T_Rev7_1-211_SA112_KVK_Rev3_1873-1898

   Plasmid

   #105635

   Purpose

   Expresses mouse monomeric Rev7 and fragment of Rev3 protein (amino acids 1873-1898) from bicistronic plasmid

   Depositor

   Thanos Halazonetis

   Inserts

   Rev7 (Mad2l2 Mouse)
   Rev3l (REV3L Human)
   

   Expression

   Bacterial

   Mutation

   Mutations: F11S, G12A, V132K, C133V, A135K

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT5T_Rev7_1-211_SA112_KVK_Rev3_1989-2014

   Plasmid

   #105637

   Purpose

   Expresses mouse monomeric Rev7 and fragment of Rev3 protein (amino acids 1989-2014) from bicistronic plasmid

   Depositor

   Thanos Halazonetis

   Inserts

   Rev7 (Mad2l2 Mouse)
   Rev3l (REV3L Human)
   

   Expression

   Bacterial

   Mutation

   Mutations: F11S, G12A, V132K, C133V, A135K

   Promoter

   T7

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT5T_Rev7_1-211_SA112_KVK_Champ1_328-355

   Plasmid

   #105638

   Purpose

   Expresses mouse monomeric Rev7 and fragment of Champ1 protein (amino acids 328-355) from bicistronic plasmid

   Depositor

   Thanos Halazonetis

   Inserts

   Rev7 (Mad2l2 Mouse)
   Champ1 (CHAMP1 Human)
   

   Expression

   Bacterial

   Mutation

   Mutations: F11S, G12A, V132K, C133V, A135K

   Promoter

   T7

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAc5.1C-Fluc-CG11206_A

   Plasmid

   #145900

   Purpose

   Insect Expression of CG11206-3UTR

   Depositor

   Elisa Izaurralde

   Article

   Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

   Insert

   CG11206-3UTR (CG11206 Fly)
   

   Expression

   Insect

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. lentiSAMv2 rs11257655-guide1

   Plasmid

   #125460

   Purpose

   CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus)

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   dCas9-VP64-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a core and U6

   Available Since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. lentiSAMv2 rs11257655-guide2

    Plasmid

    #118205

    Purpose

    CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus)

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    dCas9-VP64-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a core and U6

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. lentiSAMv2 rs11257655-guide4

    Plasmid

    #118207

    Purpose

    CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus)

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    dCas9-VP64-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a core and U6

    Available Since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. lentiSAMv2 rs11257655-guide3

    Plasmid

    #118206

    Purpose

    CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus)

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    dCas9-VP64-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a core and U6

    Available Since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE-PIK3CA-I112N

    Plasmid

    #116502

    Purpose

    Lentiviral expression of PIK3CA I112N

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Lentiviral

    Mutation

    I112N

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE-PIK3CA-E109_I112delinsD

    Plasmid

    #116468

    Purpose

    Lentiviral expression of PIK3CA E109_I112delinsD

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Lentiviral

    Mutation

    E109_I112delinsD

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28b+R3(R112C/T180C)C

    Plasmid

    #108194

    Purpose

    Expresses PdR-PCNA3(R112C/T180C)-P450cam fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PdR-PCNA3(R112C/T180C)-P450cam fusion protein
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C/T180C(PCNA3)

    Promoter

    T7 promoter

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28b+3(R112C/T180C)C

    Plasmid

    #108193

    Purpose

    Expresses PCNA3(R112C/T180C)-P450cam fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PCNA3(R112C/T180C)-P450cam fusion protein
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C/T180C(PCNA3)

    Promoter

    T7 promoter

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET15b+C3(R112C/T180C)X

    Plasmid

    #108196

    Purpose

    Expresses P450cam-PCNA3(R112C/T180C)-PdX fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    P450cam-PCNA3(R112C/T180C)-PdX fusion protein
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C/T180C(PCNA3); C73S/C85S (PdX)

    Promoter

    T7 promoter

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGS-3R112C/T180C

    Plasmid

    #85095

    Purpose

    Host vector to fuse a gene at the N-terminus of PCNA3 variant (R112C/T180C)

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    DNA polymerase sliding clamp 3
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    changed Arginine 112 to Cysteine and Threonine 18…

    Promoter

    T7 promoter

    Available Since

    Nov. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. GLYCTK gRNA (BRDN0001487112)

    Plasmid

    #77905

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human GLYCTK

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    GLYCTK (GLYCTK Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. NTRK1 gRNA (BRDN0001148112)

    Plasmid

    #75965

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human NTRK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    NTRK1 (NTRK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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