We narrowed to 73,273 results for: LAS

1. pFH2.102_PB-EPN11-MCP_I-T2A-eGFP-PGK-Blast

   Plasmid

   #205547

   Purpose

   Express EPN11-MCP-I-T2A-eGFP and blasticidin resistance marker

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   EPN11-MCP-I
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1

   Available since

   Sept. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFH2.103_PB-EPN11-MCP_p6-T2A-eGFP-PGK-Blast

   Plasmid

   #205548

   Purpose

   Express EPN11-MCP-p6-T2A-eGFP and blasticidin resistance marker

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   EPN11-MCP-p6
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMOS030E: Entry vector for: ratiometric pHluorin (endoplasmic reticulum)

   Plasmid

   #163071

   Purpose

   Entry vector for: ratiometric pHluorin (endoplasmic reticulum)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   ratiometric pHluorin
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. p2T CAG dCas9-10XGcn4-P2A-mCherry BlastR

   Plasmid

   #186745

   Purpose

   dCas9-10XGcn4-P2A-mCherry expression construct

   Depositor

   Richard Sherwood

   Article

   Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

   Insert

   Cas9-10XGcn4-P2A-mCherry
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHIE067 CAG mKate2 EF1a EBFP2-P2A-BlastR

   Plasmid

   #138747

   Purpose

   Integration Vector, assembled with pHIE041 (TUPV1), pJM451 (TUPV2), pPD612

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   CAG
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMOS031E: Entry vector for: superecliptic pHluorin (endoplasmic reticulum)

   Plasmid

   #163068

   Purpose

   Entry vector for: superecliptic pHluorin (endoplasmic reticulum)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   superecliptic pHluorin
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHIE058 ZF43x0 mKate2 EF1a EBFP2-P2A-BlastR

   Plasmid

   #138739

   Purpose

   Integration Vector, assembled with pHIE042 (TUPV1), pJM451 (TUPV2), pPD612

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   ZF43x0
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAG-FLAG-IRES-blasticidin-Sox2 S248A/T258A

   Plasmid

   #182049

   Purpose

   expression of Sox2 mutants

   Depositor

   Hyonchol Jang

   Article

   Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

   Insert

   Sox2 (Sox2 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S248A/T258A

   Promoter

   CAG

   Available since

   April 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSHS273 - Bacterial expression plasmid for SpCas9∆REC3 variant

   Plasmid

   #101202

   Purpose

   Bacterial expression plasmid for SpCas9∆REC3 variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant M1–N497,GGS,V713–D1368
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   M1–N497, GGS, V713–D1368

   Promoter

   T7

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJSC228 - Bacterial expression plasmid for SpCas9 Cluster 2 variant

    Plasmid

    #101233

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 2 variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant G582A/V583A/E584A/D585A/N588A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    G582A, V583A, E584A, D585A and N588A

    Promoter

    T7

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJSC090 - Bacterial expression plasmid for SpCas9 + K855A variant

    Plasmid

    #101213

    Purpose

    Bacterial expression plasmid for SpCas9 + K855A variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant K855A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    K855A

    Promoter

    T7

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. MDH1-miR-196a-1SM-PGK-GFP-Blast

    Plasmid

    #31510

    Depositor

    Chang-Zheng Chen

    Article

    Arnold et al Genome Res. 2011 May . 21(5):798-810.

    Insert

    miR-196a1 (Mir196a-1 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    seed mutant control

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pMXS-IRES-BLAST flag-CAD H1734A H1741A

    Plasmid

    #169830

    Purpose

    Expresses C-terminal flag-tagged CAD with mutations at reported dimerization interface of the DHOase domain

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    H1734A H1741A; TCCC -> AGTC silent mutations a…

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMXS-IRES-BLAST flag-CAD delta Loop1

    Plasmid

    #169831

    Purpose

    Expresses C-terminal flag-tagged CAD with deletion of amino acids F1366 - P1376 in allosteric domain

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Deletion of amino acids F1366 - P1376; TCCC ->…

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMXS-IRES-BLAST flag-CAD delta Loop2

    Plasmid

    #169832

    Purpose

    Expresses C-terminal flag-tagged CAD with deletion of amino acids R1398 - S1407 in allosteric domain

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Deletion of amino acids R1398 - S1407; TCCC ->…

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMXS-IRES-BLAST flag-CAD T456A S1406A

    Plasmid

    #169823

    Purpose

    Expresses C-terminal flag-tagged CAD with mutation of reported Erk1/2 and PKA phosphorylation site

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    T456A S1406A; TCCC -> AGTC silent mutations at…

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMXS-IRES-BLAST flag-CAD T456A S1859A

    Plasmid

    #169824

    Purpose

    Expresses C-terminal flag-tagged CAD with mutation of reported Erk1/2 and S6K phosphorylation site

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    T456A S1859A; TCCC -> AGTC silent mutations at…

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFH2.99_PB-EPN1-MCP_I-T2A-eGFP-PGK-Blast

    Plasmid

    #205544

    Purpose

    Express EPN1-MCP-I-T2A-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN1-MCP-I
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFH2.98_PB-EPN1-MCP_Myc-T2A-eGFP-PGK-Blast

    Plasmid

    #205543

    Purpose

    Express EPN1-MCP-Myc-T2A-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN1-MCP-Myc
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMXS-IRES-BLAST flag-CAD R1403A R1404A

    Plasmid

    #188135

    Purpose

    Expresses C-terminal flag-tagged human CAD R1403A R1404A in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge