We narrowed to 5,787 results for: PIR;

1. Str-Ii_SBP-mCherry-Golgin84

   Plasmid

   #65304

   Purpose

   synchronize trafficking of Golgin-84 from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Golgin-84 (GOLGA5 Human)
   

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. Str-Ii_SBP-EGFP-Golgin84

   Plasmid

   #65303

   Purpose

   synchronize trafficking of Golgin-84 from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Golgin-84 (GOLGA5 Human)
   

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. StrKDEL-IRES-ManII-mScarlet-i

   Plasmid

   #117274

   Purpose

   Expresses an Str-KDEL Hook for the RUSH system and separately mannosidase II-mScarlet-i

   Depositor

   David Stephens

   Article

   McCaughey et al J Cell Biol. 2018 Dec 26. pii: jcb.20180

   Inserts

   partial sequence of mannosidase II (Man2a1 Mouse)
   Streptavidin-KDEL
   

   Tags

   mScarlet-i

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 116 of ManII

   Promoter

   CMV and IRES

   Available Since

   Jan. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. EGFR-ET1

   Plasmid

   #44185

   Depositor

   Timothy Springer

   Article

   Mi et al Biochemistry. 2008 Sep 30;47(39):10314-2

   Insert

   EGFR-ET1 (EGFR Human)
   

   Tags

   3C cleavge site, protein C peptide tag, his6, SBP…

   Expression

   Mammalian

   Mutation

   I104V and D254N relative to NP_005219.2

   Promoter

   CMV

   Available Since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. Opto-GPR55

   Plasmid

   #106026

   Purpose

   Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR55; Opto-GPR55; C4)

   Depositor

   Harald Janovjak

   Article

   Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

   Insert

   Opto-GPR55 (GPR55 Bovine, Human)
   

   Tags

   Rhodopsin-1D4 and VSV-G

   Expression

   Mammalian

   Mutation

   Chimeric receptor protein

   Promoter

   CMV

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-APPL1-ΔPTB

   Plasmid

   #59768

   Purpose

   Expresses APPL1 with PTB domain deleted

   Depositor

   Donna Webb

   Article

   Broussard et al Mol Biol Cell. 2012 Apr;23(8):1486-99. d

   Insert

   APPL1 (APPL1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 467-709

   Promoter

   CMV

   Available Since

   Oct. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

7. D998-EGFR-ET1

   Plasmid

   #44186

   Depositor

   Timothy Springer

   Article

   Mi et al Biochemistry. 2008 Sep 30;47(39):10314-2

   Insert

   D998-EGFR-ET1 (EGFR Human)
   

   Tags

   3C cleavge site, protein C peptide tag, his6, SBP…

   Expression

   Mammalian

   Mutation

   EGFR amino acids 1−998

   Promoter

   CVM

   Available Since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

8. MAP1LC3A-V1

   Plasmid

   #98569

   Purpose

   Expresses MAP1LC3A-V1

   Depositor

   Shahenda El-Naggar

   Article

   Nassar et al Sci Rep. 2017 Aug 14;7(1):8022. doi: 10.

   Insert

   MAP1LC3A (MAP1LC3A Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC19-Q0P897

   Plasmid

   #103120

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   Q0P897(Cas9 coding gene from Rhodospirillum rubrum (strain ATCC 11170 / NCIB 8255))
   

   Use

   CRISPR; Cloning vector

   Mutation

   human codon-optimized

   Available Since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC19-D3NT09

    Plasmid

    #103074

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    D3NT09(Cas9 coding gene from Azospirillum sp. (strain B510))
    

    Use

    CRISPR; Cloning vector

    Mutation

    human codon-optimized

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. p35S-FHA-SpAGO4a_gDNA

    Plasmid

    #216841

    Purpose

    N-term Flag-HA tagged Spirodela polyrhiza (Sp9509) AGO4a gDNA under regulation of CaMV 35S promoter

    Depositor

    Arturo Marí-Ordóñez

    Article

    Dombey et al bioRxiv 2024.04.03.587901; doi: https://

    Insert

    ARGONAUTE4
    

    Tags

    Flag-HA

    Expression

    Plant

    Promoter

    CaMV 35S

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. p35S-FHA-SpAGO4a_cDNA

    Plasmid

    #216842

    Purpose

    N-term Flag-HA tagged Spirodela polyrhiza (Sp9509) AGO4a cDNA under regulation of CaMV 35S promoter

    Depositor

    Arturo Marí-Ordóñez

    Article

    Dombey et al bioRxiv 2024.04.03.587901; doi: https://

    Insert

    ARGONAUTE4
    

    Tags

    Flag-HA

    Expression

    Plant

    Promoter

    CaMV 35S

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. GPR55-Tango

    Plasmid

    #66363

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR55 (GPR55 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

14. Str-STIM1-NN_neomycin

    Plasmid

    #65311

    Purpose

    Luminal ER hook only (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    STIM1 mutated for binding motif to microtubule (STIM1 Human)
    

    Tags

    streptavidin

    Expression

    Mammalian

    Mutation

    2 amino acids of STIM1 (IP from SxIP motif) were …

    Promoter

    CMV

    Available Since

    June 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. Cx32R220stop

    Plasmid

    #78151

    Purpose

    Mammalian Expression of the first 219 amino acids of Connexin 32 and EGFP from a bicistronic mRNA

    Depositor

    Mario Bortolozzi

    Article

    Carrer et al Hum Mol Genet. 2017 Oct 25. pii: 4565496

    Insert

    Connexin 32 (GJB1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Only contains first 219 residues

    Promoter

    CMV

    Available Since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

16. Str-STIM1-NN_puromycin

    Plasmid

    #65310

    Purpose

    Luminal ER hook only (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    STIM1 mutated for binding motif to microtubule (STIM1 Human)
    

    Tags

    streptavidin

    Expression

    Mammalian

    Mutation

    2 amino acids of STIM1 (IP from SxIP motif) were …

    Promoter

    CMV

    Available Since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-N1 VAMP2 R125TAG

    Plasmid

    #69876

    Purpose

    express in mammalian cells for unnatural amino acid incorporation into VAMP2-GFP

    Depositor

    Silvio Rizzoli

    Article

    Vreja et al ACS Nano. 2015 Nov 24;9(11):11034-41. do

    Insert

    VAMP2-GFP (Vamp2 Rat)
    

    Use

    Synthetic Biology

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Amber stop codon in the linker between VAMP2 and …

    Available Since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

18. GFP-APPL1-R146A/K152A/R154A

    Plasmid

    #59767

    Purpose

    Expresses APPL1 Endosomal Localization Mutant

    Depositor

    Donna Webb

    Article

    Broussard et al Mol Biol Cell. 2012 Apr;23(8):1486-99. d

    Insert

    APPL1 (APPL1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    R146A/K152A/R154A

    Promoter

    CMV

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pN1 VAMP2 R125TAG

    Plasmid

    #69877

    Purpose

    express in mammalian cells for unnatural amino acid incorporation into VAMP2

    Depositor

    Silvio Rizzoli

    Article

    Vreja et al ACS Nano. 2015 Nov 24;9(11):11034-41. do

    Insert

    VAMP2 (Vamp2 Rat)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Mutation

    Amber and Ochre stop codons in the former linker …

    Available Since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pTE4561

    Plasmid

    #107525

    Purpose

    Expresses Lb crRNA and mCherry in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Lb crRNA
    mCherry
    

    Expression

    Mammalian

    Promoter

    CBh and human U6

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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