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psicheck-2-Mut

Plasmid

#48164

Purpose

contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of Mut

Use

Luciferase

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only
pJM614-TRA-2::GFP

Plasmid

#27128

Depositor

Ding Xue

Article

Mapes et al Proc Natl Acad Sci U S A. 2010 Oct 19. 1

Insert

TRA-2 GFP (tra-2 Nematode)

Tags

GFP

Expression

Worm

Mutation

replaced stop codon with AgeI and EcoRI added GFP…

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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GST HP1 gamma deltaC

Plasmid

#24077

Depositor

Naoko Tanese

Article

Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

Insert

HP1 gamma (CBX3 Human)

Tags

GST

Expression

Bacterial

Mutation

Missing the C-terminal 25 amino acids

Available Since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
HL 4175 (=HL 716 + pHL 1391)

Plasmid

#30096

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLlacO-1:rpoS::gfp

Use

Synthetic Biology

Tags

GFP

Expression

Bacterial

Available Since

July 11, 2011

Availability

Academic Institutions and Nonprofits only
pET-21a-GFP-Patronin CC 1288–1457

Plasmid

#59055

Purpose

for E. coli expression of Patronin CC truncation

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

Patronin (Patronin Fly)

Tags

GFP

Expression

Bacterial

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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TU#1448_unc-73b_cDNA

Plasmid

#82362

Purpose

express unc-73b cDNA

Depositor

Martin Chalfie

Article

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

unc-73 b isoform (unc-73 Nematode)

Expression

Worm

Promoter

none

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pJLI-Sup35?M

Plasmid

#1225

Depositor

Susan Lindquist

Article

Liu et al Proc Natl Acad Sci U S A 2002 Dec 10;99

Insert

SUP35 (SUP35 Budding Yeast)

Use

Yeast integrative plasmid

Mutation

deletion of aa 124-253 of SUP35

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pHL 1011

Plasmid

#52993

Purpose

pLlac0-1:RhyB-mCherry, pLtetO-1:RBS (st7) gfp

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Inserts

RhyB sRNA
RBS (st7)

Use

Synthetic Biology

Tags

GFP and RBS-mCherry

Expression

Bacterial

Promoter

pLlac0-1 and pLtetO-1

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only
140_pSKB3-GBD-LUMP

Plasmid

#65972

Purpose

Bacterial expression of GBD-LUMP protein, a sensor for GTP-bound Cdc42

Depositor

Gerard Marriott

Article

Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p

Insert

LUMP

Tags

GBD (GTPase binding domain from ACK1; residues 44…

Expression

Bacterial

Promoter

T7

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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mg172

Plasmid

#34652

Depositor

Miriam Goodman

Article

Johnson et al Proc Natl Acad Sci U S A. 2011 Nov 2.

Insert

slo-1(A1;B1;C0) (slo-1 Nematode)

Use

Xenopus oocyte expression

Tags

Xenopus beta-globulin 3' UTR and Xenopus bet…

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pHL1575

Plasmid

#53028

Purpose

PconNoHind:csrB, PLtetO-1:RBS(st3):csrD

Depositor

Han Lim

Article

Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

Inserts

csrB
csrD

Use

Synthetic Biology

Tags

RBS (st3)

Expression

Bacterial

Promoter

PconNoHind and pLtetO-1

Available Since

May 14, 2014

Availability

Academic Institutions and Nonprofits only
pHL1756

Plasmid

#53036

Purpose

PconNoHindM12:glgC::gfp, PconNoHind:RBS(st3):tetR

Depositor

Han Lim

Article

Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

Inserts

GFP
tetR

Use

Synthetic Biology

Tags

RBS(st3) and glgC leader

Expression

Bacterial

Promoter

pConNoHind and pConNoHindM12

Available Since

May 14, 2014

Availability

Academic Institutions and Nonprofits only
HL 3152 (=HL 716 + pHL 1056)

Plasmid

#30048

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pCon:MicC, pLlacO-1:rpoS::gfp

Use

Synthetic Biology

Tags

GFP

Expression

Bacterial

Available Since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only
pCR.MCV.Ori339 (589)

Plasmid

#28192

Depositor

Patrick Moore

Article

Shuda et al Proc Natl Acad Sci U S A. 2008 Oct 21. 1

Insert

MCV replication region

Expression

Bacterial

Available Since

Oct. 3, 2011

Availability

Academic Institutions and Nonprofits only
HL 4180 (=HL 2752 + pHL 1358)

Plasmid

#30099

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLlacO-1:hfq, pLtetO-1m9:sodB::gfp

Use

Synthetic Biology

Tags

GFP

Expression

Bacterial

Available Since

Sept. 9, 2011

Availability

Academic Institutions and Nonprofits only
MZB139

Plasmid

#217838

Purpose

Empty 10xHis-SUMO (Smt3) vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression

Depositor

Richard Baker

Article

Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

SMT3 (SMT3 Budding Yeast)

Tags

10x His and SUMO (SMT3)

Expression

Bacterial

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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MZB164

Plasmid

#217842

Purpose

10xHis-SUMO (Smt3) fused, HRV-3C cleavable, Halotag for rapid cloning/DNA assembly of pulldown targets via SwaI restriction site for E. coli based protein expression

Depositor

Richard Baker

Article

Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

HaloTag

Tags

10x His, HaloTag, and SUMO (SMT3)

Expression

Bacterial

Promoter

T7 Promoter

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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MZB216

Plasmid

#221766

Purpose

"Empty" C-terminal CPD-10xHis vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression

Depositor

Richard Baker

Article

Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

rtxA

Promoter

T7 Promoter

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-MLG-EGFP-LST2

Plasmid

#220785

Purpose

Expresses N-terminal EGFP-LST2 in mammalian cells

Depositor

Timm Maier

Article

Battaglioni et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

LST2 (ZFYVE28 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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STREP-SUMO-FLAG-SeKaiC

Plasmid

#218179

Purpose

WT SeKaiC for phosphorylation/dephosphorylation study

Depositor

Andy LiWang

Article

Kim et al Proc Natl Acad Sci U S A. 2008 Sep 2;105

Insert

STREP-SUMO-FLAG-SeKaiC

Tags

STREP-Tag

Expression

Bacterial

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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