We narrowed to 7,660 results for: ski

1. NLS-dCas9-HA-NLS--GFP-p300

   Plasmid

   #183925

   Purpose

   p300 HAT core domain coupled to catalytically dead dCas9 for localization; labeled with GFP

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   dCas9-GFP-p300
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CMV-eBFP2-NLS-pA

   Plasmid

   #183800

   Purpose

   AAV vector to express nuclear localized eBFP2 from CMV promoter

   Depositor

   Jonathan Ploski

   Article

   de Solis et al Brain Res. 2017 Oct 1;1672:148-162. doi:

   Insert

   eBFP2-NLS
   

   Use

   AAV

   Tags

   NLS

   Available Since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-tight-DDdCas9GFP-IRES-Neo

   Plasmid

   #102907

   Purpose

   PiggyBac construct with doxycyline inducible TRE-tight promoter expression of dCas9-GFP followed by IRES-Neomycin selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   DDdCas9GFP-IRES-Neo
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   TRE-tight

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. CAG-SaCas9-WPRE

   Plasmid

   #89996

   Purpose

   Expresses high levels of WT SaCas9 for genome editing

   Depositor

   Timo Otonkoski

   Article

   Balboa et al Stem Cell Res. 2017 Jul;22:16-19. doi: 1

   Insert

   SaCas9 (NEWENTRY Staphylococcus aureus)
   

   Tags

   3xHA, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. NLS-tdPCP-GFP-VPR

   Plasmid

   #183936

   Purpose

   PCP tandem dimer binding to PP7 RNA stem loops coupled to VP16 activation domain; labeled with GFP

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   tdPCP-GFP-VPR
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV (TATA box removed)

   Available Since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHP138 Myc-TRF2-deltaB deltaM

   Plasmid

   #164255

   Purpose

   for generation of mammalian cells with inducible expression of dominant negative TRF2 for induction of telomere crisis used together with any PiggyBac transposase plasmid

   Depositor

   John Maciejowski

   Article

   Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

   Insert

   TRF2
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   deletion of amino acids-86 and 496-542

   Promoter

   Tetracycline inducible promotor

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO.1 shSV40 neo

   Plasmid

   #158646

   Purpose

   Lentiviral shRNA vector targeting simian virus 40 T antigen (SV40Tag)

   Depositor

   Kevin Janes

   Article

   Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

   Insert

   SV40Tag (SV40gp6 Simian virus 40)
   

   Use

   Lentiviral

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. FKBP-myc

   Plasmid

   #20211

   Depositor

   Carolyn Bertozzi

   Article

   Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

   Insert

   FKBP (FKBP1A Human)
   

   Tags

   myc

   Expression

   Mammalian

   Available Since

   Feb. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pscALPS- HIV2-ROD vpx

   Plasmid

   #115816

   Purpose

   Encodes codon optimized 3xFLAG-HIV2ROD Vpx (M15390) and puromycin resistance protein

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   HIV2ROD Vpx (M15390)
   

   Use

   Lentiviral

   Tags

   3x-FLAG

   Promoter

   SFFV

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCellFree_G03 CIRBP

    Plasmid

    #67055

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CIRBP (CIRBP Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

11. NLS-dCas9-HA-NLS-GFP-VP16

    Plasmid

    #183923

    Purpose

    VP16 activation domain coupled to catalytically inactive dCas9 for localization; labeled with GFP

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    dCas9-GFP-VP16
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBS PKC theta

    Plasmid

    #8426

    Depositor

    Frederic Mushinski

    Article

    Mischak et al FEBS Lett 1993 Jul 12;326(1-3):51-5.

    Insert

    Mouse Protein Kinase C theta (Prkcq Mouse)
    

    Expression

    Bacterial

    Available Since

    Nov. 22, 2005

    Availability

    Academic Institutions and Nonprofits only

13. pLX302 OAS2-V5 puro

    Plasmid

    #158642

    Purpose

    Lentiviral expression vector for constitutive expression of V5-tagged OAS2.

    Depositor

    Kevin Janes

    Article

    Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

    Insert

    OAS2 (OAS2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCellFree_G03 IL6

    Plasmid

    #67061

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    IL6 (IL6 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pQCXIZ-turq-3xFLAG-TREX1 D18N

    Plasmid

    #164233

    Purpose

    retroviral plasmid for turq-3xFLAG-TREX1 D18N (catalytically-deficient)

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    TREX1 (TREX1 Human)
    

    Use

    Retroviral

    Tags

    FLAG and mturquoise

    Expression

    Mammalian

    Mutation

    D18N ->nuclease deficient

    Promoter

    CMV

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL3_5UTR_Myc_FLuc

    Plasmid

    #229502

    Purpose

    Myc 5' untranslated region firefly luciferase reporter.

    Depositor

    Davide Ruggero

    Article

    Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

    Insert

    Myc 5'UTR
    

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pscALPS-HIV-1 Group P-vpr

    Plasmid

    #115837

    Purpose

    Encodes codon optimized 3xFLAG-HIV1 Group P Vpr (HQ179987) and puromycin resistance protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    HIV1 Group P Vpr (HQ179987)
    

    Use

    Lentiviral

    Tags

    3x-FLAG

    Promoter

    SFFV

    Available Since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. RhoC FLARE.sc mCer, mVenus - wt

    Plasmid

    #65071

    Purpose

    RhoC biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC

    Depositor

    Klaus Hahn

    Article

    Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

    Insert

    RBD, mCerulean, mVenus, RhoC
    

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    T153M in mVenus

    Promoter

    CMV, p10

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pFA6a-link-GFPEnvy-SpHis5

    Plasmid

    #60782

    Purpose

    Yeast C-terminal tagging vector, which creates gene fusions with GFPEnvy using SpHis5 selection

    Depositor

    Linda Huang

    Article

    Slubowski et al Yeast. 2015 Jan 22. doi: 10.1002/yea.306

    Insert

    GFPEnvy
    

    Use

    Yeast integrating

    Mutation

    S30R Y39N T65C S72A F99S N105T Y145F M153T V163A …

    Available Since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMTH PKC alpha

    Plasmid

    #8409

    Depositor

    Frederic Mushinski

    Article

    Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

    Insert

    Mouse Protein Kinase C alpha (Prkca Mouse)
    

    Expression

    Mammalian

    Available Since

    March 5, 2007

    Availability

    Academic Institutions and Nonprofits only