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Plasmid#187186PurposeProtein ExpressionDepositorInsertENSDARG00000024546 (pla2g4aa Zebrafish)
UseTagsEGFPExpressionBacterialMutationY90F, V91FPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
NLS-GI-VP16-IRES-NLS-GAL4DBD-GID-SmBiT-FKF1
Plasmid#183404PurposeDual expression of GI-VP16 and GAL4DBD-GID-SmBiT-FKF1 in the nucleusDepositorInsertGI-VP16-IRES-GAL4DBD-GID-SmBiT-FKF1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
NLS-GI-VP16-IRES-NLS-GAL4DBD-NanoLuc-FKF1
Plasmid#183399PurposeDual expression of GI-VP16 and GAL4DBD-NanoLuc-FKF1 in the nucleusDepositorInsertGI-VP16-IRES-GAL4DBD-NanoLuc-FKF1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pJH2198
Plasmid#179773PurposePrgef-1 NLF-1::GFP unc-54 3' UTR C.elegans pan-neural expression of NLF-1::GFPDepositorInsertNLF-1::GFP (nlf-1 Nematode)
UseTagsGFPExpressionWormMutationPromoterPrgef-1Available sinceMarch 14, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pET11a-Z-N-wtBlc
Plasmid#168258PurposeN-fragment of the wtBlc‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168256: pMRBad-Z-C-wtBlc to obtain wtBlc‐split-Zip proteinDepositorInsertN-fragment of wtBlc + Leucine Zipper
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRBad-C-wtBlc
Plasmid#168257PurposeC-fragment. This plasmid can be co-transformed with either Plasmid 168259: pET11a-N-wtBlc or Plasmid 168472: pET11a-N-DiB2 to obtain wtBlc‐split or DiB2‐split protein, correspondinglyDepositorInsertC-fragment of wtBlc
UseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRBad-Z-C-wtBlc
Plasmid#168256PurposeC-fragment. This plasmid can be co-transformed with either Plasmid 168258: pET11a-Z-N-wtBlc or Plasmid 168473: pET11a-Z-N-DiB2 to obtain wtBlc‐split-Zip or DiB2‐split-Zip protein, correspondinglyDepositorInsertLeucine Zipper + C-fragment of wtBlc
UseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc120
Plasmid#161950PurposeContains elements for building reporter plasmid for IBM. Built upon pSB4A3. ECF20 reporter plasmidDepositorInsertpSB4A3-PECF20-B30-mScarlet-I-B15
UseTagsExpressionBacterialMutationPromoterPECF20Available sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc393
Plasmid#161953PurposeDemonstrates chemically induced dimmerization of acVHH. T7 RNAP polymerase reporter plasmidDepositorInsertpSB4A3-T-PT7-B30-mScarlet-I-3T
UseTagsExpressionBacterialMutationPromoterPT7Available sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc330
Plasmid#161952PurposeContains elements for building reporter plasmid for IBM. SrpR reporter plasmidDepositorInsertpSEVA171-PSrpR-B30-mScarlet-I-Ter
UseTagsExpressionBacterialMutationPromoterPSrpRAvailable sinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc050
Plasmid#161938PurposeCarries Golden Gate substitution insert for IBM with M86 intein. Plux2 as C-lobe promoterDepositorInsertpSB1A3-BbsI-M86N-cat-Plux2-B32-M86C-SapI
UseTagsExpressionBacterialMutationPromoterPlux2Available sinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc259
Plasmid#161951PurposeContains elements for building reporter plasmid for IBM.TetR reporter plasmidDepositorInsertpSEVA471-PTet*-RiboJ10-B64-mScarlet-I-Ter
UseTagsExpressionBacterialMutationPromoterPTet*Available sinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
px330 WAPL gRNA
Plasmid#165595PurposeInsertion of WAPL degromDepositorInsertWAPL (Wapl Mouse)
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
px330 BRM gRNA
Plasmid#165586PurposeInsertion of BRM degronDepositorInsertBRM (Smarca4 Mouse)
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFlox_N-BRM_BSD_FKBPF36V_EYFPF36V
Plasmid#163876PurposeAcute Depletion of BRM-EYFPDepositorInsertBrm (Smarca2 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_Hsa-Serum Albumin SP
Plasmid#153496PurposeHuman Serum Albumin Signal Peptide Donor PlasmidDepositorInsertSerum Albumin SP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_Sce-Killer Protein SP
Plasmid#153498PurposeS. cerevisiae Killer Signal Peptide Donor PlasmidDepositorInsertKiller Protein SP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_Cfo-CPO SP
Plasmid#153500PurposeCaldariomyces (Leptoxyphium) fumago Chloroperoxidase Signal Peptide Donor PlasmidDepositorInsertChloroperoxidase SP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only