We narrowed to 39,931 results for: LAT;

1. GFP-CLCb (EED/QQN)

   Plasmid

   #47422

   Depositor

   Peter McPherson

   Article

   Poupon et al Proc Natl Acad Sci U S A. 2008 Jan 8;105

   Insert

   CLCb EED/QQN (CLTB Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   EED changed to QQN, in aa20-41 region

   Promoter

   CMV

   Available Since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pGEX-2T-TRR-C421-Y2404F

   Plasmid

   #182846

   Purpose

   mutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 2011-2431 aa). Expression in E. coli. by IPTG induction

   Depositor

   Peter Harte

   Article

   Tie et al Development. 2014 Mar;141(5):1129-39. do

   Insert

   TRR 2011-2431 (trr Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   mutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 20…

   Promoter

   Available Since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pShuttle-EGFP-CLASP2 340-1362 9xS/A

   Plasmid

   #24373

   Depositor

   Torsten Wittmann

   Article

   Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

   Insert

   CLASP2 (340-1362) 9xS/A (CLASP2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Nonphosphorylatable CLASP2 deletion mutant. M…

   Promoter

   Available Since

   June 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAdEasy-EGFP-CLASP2 340-1084 9xS/A

   Plasmid

   #24525

   Depositor

   Torsten Wittmann

   Article

   Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

   Insert

   CLASP2 (340-1084) 9xS/A (CLASP2 Human)
   

   Use

   Adenoviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Nonphosphorylatable CLASP2 deletion mutant. M…

   Promoter

   Available Since

   June 29, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pShuttle-EGFP-CLASP2 340-1084 9xS/A

   Plasmid

   #24384

   Depositor

   Torsten Wittmann

   Article

   Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

   Insert

   CLASP2 (340-1084) 9xS/A (CLASP2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Nonphosphorylatable CLASP2 deletion mutant. M…

   Promoter

   Available Since

   June 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. SAM-DNMT3A-inactive+sgGFP

   Plasmid

   #213169

   Purpose

   Vector with sgGFP used as a negative control (inactive DNMT3A) for induction of global DNA methylation.

   Depositor

   Sefi Rosenbluh

   Article

   Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

   Insert

   sgGFP
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   E1Fa and U6

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. SAM-DNMT3A-inactive+sgAAVS1_145

   Plasmid

   #213170

   Purpose

   Vector with sgAAVS1 used as a negative control (inactive DNMT3A) for induction of global DNA methylation.

   Depositor

   Sefi Rosenbluh

   Article

   Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

   Insert

   sgAAVS1_145
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   E1Fa and U6

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX-2T-TRR-C421-G2304D

   Plasmid

   #182845

   Purpose

   mutation G2304D (GGC/GAC), PCR product coding C-terminal residues 2011-2431 of TRR was inserted into modified pGEX-2T by Nde I-Nsi I sites

   Depositor

   Peter Harte

   Article

   Tie et al Development. 2014 Mar;141(5):1129-39. do

   Insert

   TRR 2011-2431 (trr Fly)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   mutation G2304D (GGC/GAC)

   Promoter

   Available Since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-CLASP2 512-650 9xS/A

   Plasmid

   #24410

   Depositor

   Torsten Wittmann

   Article

   Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

   Insert

   CLASP2 (512-650) 9xS/A (CLASP2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Nonphosphorylatable CLASP2 deletion mutant that c…

   Promoter

   Available Since

   April 23, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. DNA-PKcs PQR>ala

    Plasmid

    #83319

    Purpose

    human DNA-PKcs PQR>ala

    Depositor

    Katheryn Meek

    Article

    Cui et al Mol Cell Biol. 2005 Dec;25(24):10842-52.

    Insert

    human DNA-PKcs (PRKDC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    5 phosphorylation sites in the PQR cluster substi…

    Promoter

    Available Since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. DNA-PKcs ABCDE>A

    Plasmid

    #83318

    Purpose

    human DNA-PKcs/ABCDE>ala

    Depositor

    Katheryn Meek

    Article

    Cui et al Mol Cell Biol. 2005 Dec;25(24):10842-52.

    Insert

    human DNA-PKcs (PRKDC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    6 phosphorylation sites in the ABCDE cluster subs…

    Promoter

    Available Since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. RAPpGEX

    Plasmid

    #162241

    Purpose

    Expresses LDL-receptor associated protein (RAP), full length in mammalian cells

    Depositor

    Andreas Faissner

    Article

    Hennen et al J Biol Chem. 2013 Jun 7;288(23):16538-45

    Insert

    lrpap1 (Lrpap1 Mouse)
    

    Use

    Tags

    GST-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCasper4-g-osk-TRICK

    Plasmid

    #71244

    Purpose

    oskar translation biosensor with 12x copies of modified PP7 stem loops and 6xMS2 stem loops. . full genomic region, used for P element-mediated germline transformation in Drosophila.

    Depositor

    Anne Ephrussi

    Article

    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

    Insert

    oskar (osk Fly)
    

    Use

    P element-mediated germline transformation

    Tags

    12xPP7 and 6xMS2

    Expression

    Insect

    Mutation

    Promoter

    osk

    Available Since

    Dec. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA6.2-YFP-CWT

    Plasmid

    #176689

    Purpose

    Fluorescent reporter for phagosomal escape of Staphylococcus aureus

    Depositor

    Martin Fraunholz

    Article

    Blattner et al PLoS Pathog. 2016 Sep 15;12(9):e1005857.

    Insert

    cell wall-targeting domain of Lysostaphin
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    deletion of protase domain of Lysostaphin

    Promoter

    CMV

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLIK-NFLAG-hFUS

    Plasmid

    #50487

    Purpose

    Produces lentivirus expressing human FUS with FLAG tag at its N-terminus by co-transfection with psPAX2 and pMD2G

    Depositor

    Zissimos Mourelatos

    Article

    Nakaya et al RNA. 2013 Apr;19(4):498-509. doi: 10.126

    Insert

    FUS (FUS Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    TRE

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3-mCherry-TSC2(AA,3Q)-NLS

    Plasmid

    #192445

    Purpose

    Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP activity; targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    mCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q )-NLS (TSC2 Human)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. ECON

    Plasmid

    #82553

    Purpose

    Epigenetic effector construct for targeted methylation

    Depositor

    Joseph Irudayaraj

    Article

    Choudhury et al Oncotarget. 2016 Jul 4. doi: 10.18632/on

    Inserts

    DNMT3A (DNMT3A Human)
    CRY2 (CRY2 Mustard Weed)
    

    Use

    Tags

    HALO and mCherry

    Expression

    Mammalian

    Mutation

    N-terminal photolyase homology region domain of C…

    Promoter

    CMV

    Available Since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMIR-Report-Luc-KLF4-K1S

    Plasmid

    #62979

    Purpose

    Translational reporter for KLF4

    Depositor

    Michael Ruppert

    Article

    Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

    Insert

    KLF4 partial sequence (KLF4 Human)
    

    Use

    Luciferase

    Tags

    firefly luciferase

    Expression

    Mutation

    Fragment 1-446 of KLF4

    Promoter

    CMV

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

19. SAM-DNMT3A-inactive+sgAAVS1_118

    Plasmid

    #213171

    Purpose

    Vector with sgAAVS1 used as a negative control (inactive DNMT3A) for induction of global DNA methylation.

    Depositor

    Sefi Rosenbluh

    Article

    Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

    Insert

    sgAAVS1_118 (AAVS1 Human)
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa and U6

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJET-osr1-KI-donor

    Plasmid

    #184875

    Purpose

    Donor template for knock-in of p2a-CreERT2 into the medaka osr1 locus via CRISPR/Cas9-mediated homology directed repair

    Depositor

    Christoph Winkler

    Article

    Hui et al Development. 2022 May 20. pii: 275483. d

    Inserts

    Odd-Skipped Related Transcription Factor 1 5' homology arm
    Odd-Skipped Related Transcription Factor 1 3' homology arm
    p2a-CreERT2
    Myosin light chain 2 promoter
    EGFP
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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