We narrowed to 74,860 results for: Rest

1. pEVOL-NBK-1

   Plasmid

   #207639

   Purpose

   A plasmid for the expression of M. mazei pyrrolysine aminoacyl-tRNA synthetase/tRNA pair (PylRS/tRNAPyl) in E. Coli.

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   Pyl-tRNACUA and 2x pyrrolysyl-tRNA synthetase (MmPylRS)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   The evolved synthetase NBK-1 contains mutations Y…

   Promoter

   araBAD

   Available since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOPINK-OTUD3 (OTU, aa 52-209)

   Plasmid

   #61412

   Purpose

   Expresses human OTUD3 (OTU domain) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD3 (OTUD3 Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-51 and aa 210-398.

   Promoter

   T7

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3_HMOX1-V5-APEX2

   Plasmid

   #85823

   Purpose

   Expressed C-term tagged APEX2 on HMOX1 in mammalian cell

   Depositor

   Hyun-Woo Rhee

   Article

   Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

   Insert

   HMOX1-V5-APEX2 (HMOX1 Human, Synthetic)
   

   Use

   Tags

   APEX2 and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-F

   Available since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. p1192-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS

   Plasmid

   #129530

   Purpose

   Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme

   Depositor

   Erik Sontheimer

   Article

   Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

   Inserts

   codon-optimized AcrIIC3Nme
   sgRNA_Rosa26
   

   Use

   AAV

   Tags

   FLAG/NLS

   Expression

   Mutation

   Promoter

   U6 and cytomegalovirus-enhancer chicken β-actin p…

   Available since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOPINK-OTUD1 (OTU+UIM, aa 287-481)

   Plasmid

   #61406

   Purpose

   Expresses human OTUD1 (OTU+UIM domains) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD1 (OTUD1 Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-286.

   Promoter

   T7

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3_V5-APEX2-Sec61B

   Plasmid

   #83412

   Purpose

   Expressed N-term tagged APEX2 on Sec61B in mammalian cell

   Depositor

   Hyun-Woo Rhee

   Article

   Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

   Insert

   V5-APEX2-Sec61B (SEC61B Human, Synthetic)
   

   Use

   Tags

   APEX2 and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-F

   Available since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. Fz4-GFP

   Plasmid

   #42197

   Depositor

   Robert Lefkowitz, Jeremy Nathans

   Article

   Chen et al Science. 2003 Sep 5;301(5638):1391-4.

   Insert

   Frizzled 4 (Fzd4 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmiRGLO Dual-Luciferase-PIK3R1-3'UTR

   Plasmid

   #182252

   Purpose

   Human PIK3R1 3` UTR region containing 1x binding site for miR-150 cloned downstream of luciferase reporter gene

   Depositor

   Silvia Monticelli

   Article

   Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

   Insert

   3' UTR region of PIK3R1 gene
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. 1119 pBabe puroL HA FKHR AAA

   Plasmid

   #9025

   Depositor

   William Sellers

   Article

   Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

   Insert

   FKHR AAA (FOXO1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   T24A S256A S319A

   Promoter

   Available since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pET14b-PITPalpha

    Plasmid

    #58256

    Purpose

    bacterial expression of human PITP alpha

    Depositor

    Shamshad Cockcroft

    Article

    Hara et al J Biol Chem. 1997 Jun 6;272(23):14908-13

    Insert

    PITP alpha (PITPNA Human)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 28, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pCW-OVOL2-HA

    Plasmid

    #184737

    Purpose

    Doxycycline-inducible lentiviral expression of OVOL2

    Depositor

    Gloria Manzotti, Alessia Ciarrocchi

    Article

    Gugnoni et al J Exp Clin Cancer Res. 2022 Mar 25;41(1)

    Insert

    ovo like zinc finger 2 (OVOL2 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Tet ON

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOP/SSAT

    Plasmid

    #140291

    Purpose

    Expresses human SSAT in bacterial cells

    Depositor

    Saverio Bettuzzi, Gaetano Marverti

    Article

    Marverti et al Carcinogenesis. 2005 Oct;26(10):1677-86.

    Insert

    SSAT1 (SAT1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hNR2F2-RFP

    Plasmid

    #22926

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 . 3():19.

    Insert

    human nuclear receptor subfamily 2 group F member 2 promoter driving DsRedExpress (NR2F2 Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pscAAV-Tyr

    Plasmid

    #135616

    Purpose

    Donor vector for genomic targeting of an EcoRI restriction site to the mouse Tyrosinase locus

    Depositor

    Lin He

    Article

    Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e

    Insert

    EcoRI restriction site flanked by Tyr homology arms (Tyr Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. RnBarr1-mVenus

    Plasmid

    #137790

    Purpose

    Visualization of beta-arrestin1

    Depositor

    Dorus Gadella

    Article

    Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

    Insert

    Rat beta-arrestin 1 (Arrb1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSEMS-SDHB-Halo7Tag

    Plasmid

    #108973

    Purpose

    Selflabeling tag at succinate dehydrogenase complex subunit B (SDHB)

    Depositor

    Karin Busch

    Article

    Wilkens et al J Cell Sci. 2013 Jan 1;126(Pt 1):103-16.

    Insert

    succinate dehydrogenase complex iron sulfur subunit B (SDHB Human)
    

    Use

    Tags

    Halo7-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. p1194-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIA4_FLAG_NLS

    Plasmid

    #129532

    Purpose

    Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIA4

    Depositor

    Erik Sontheimer

    Article

    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

    Inserts

    codon-optimized AcrIIA4
    sgRNA_Rosa26
    

    Use

    AAV

    Tags

    FLAG/NLS

    Expression

    Mutation

    Promoter

    U6 and cytomegalovirus-enhancer chicken β-actin p…

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOPINK-OTUD5 (OTU, aa 171-358)

    Plasmid

    #61415

    Purpose

    Expresses human OTUD5 (OTU domain) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD5 (OTUD5 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-170 and aa 359-571.

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOPINK-OTUD3 (OTU+UBA, aa 52-275)

    Plasmid

    #61411

    Purpose

    Expresses human OTUD3 (OTU+UBA domains) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD3 (OTUD3 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-51 and aa 276-398.

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Th-tdTomato

    Plasmid

    #159460

    Purpose

    Targeting vector to TH gene locus for induction of P2A-fused tdTomato, with floxed PGK-puroR drug resistant cassette

    Depositor

    Yuejun Chen

    Article

    Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-

    Insert

    P2A-tdTomato
    

    Use

    Cre/Lox

    Tags

    P2A

    Expression

    Mammalian

    Mutation

    Promoter

    Endogenous TH

    Available since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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