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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,980 results for: SEC

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Showing: 1221 - 1240 of 10980 results

  2. pOCC102

    Plasmid
    #118883
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C protease
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6-MBP, cleavable with 3C protease
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118883-plasmid-map-sequence-id-232315

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  3. pYSD080

    Plasmid
    #212917
    Purpose
    Encodes the S. cerevisiae Aga1p yeast surface display anchor protein as a Type 3 part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)
    Depositor
    Paul Young
    Article
    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102
    Insert
    AGA1 (AGA1 Budding Yeast)
    Expression
    Bacterial
    Available Since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    212917-plasmid-map-sequence-id-421793

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  4. pYSD083

    Plasmid
    #212920
    Purpose
    Encodes the HA-tagged S. cerevisiae Sed1p yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)
    Depositor
    Paul Young
    Article
    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102
    Insert
    SED1 (SED1 Budding Yeast)
    Expression
    Bacterial
    Available Since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    212920-plasmid-map-sequence-id-421785

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  6. pYSD023

    Plasmid
    #212909
    Purpose
    Encodes a hybrid S. cerevisiae OST1 pre- MFalpha1 pro- signal peptide (OST1MFapp) as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)
    Depositor
    Paul Young
    Article
    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102
    Insert
    MFalpha1 (MF(ALPHA)1 Budding Yeast)
    Expression
    Bacterial
    Available Since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    212909-plasmid-map-sequence-id-421774

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  7. pYSD081

    Plasmid
    #212918
    Purpose
    Encodes the HA-tagged S. cerevisiae Aga2p yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)
    Depositor
    Paul Young
    Article
    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102
    Insert
    AGA2 (AGA2 Budding Yeast)
    Expression
    Bacterial
    Available Since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    212918-plasmid-map-sequence-id-421770

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  12. pECIA2

    Plasmid
    #47032
    Purpose
    This is a Gateway destination vector for secreted expression in Drosophila cell culture by induction via CuSO4. Inserts will be C-terminally tagged with Fc, V5 and hexahistidine tags.
    Depositor
    Chris Garcia
    Article
    Ozkan et al Cell. 2013 Jul 3;154(1):228-39. doi: 10.
    Type
    Empty backbone
    Use
    Secreted expression in drosophila culture
    Tags
    Fc(human IgG1), HRV 3C Protease Cleavage site, He…
    Expression
    Insect
    Promoter
    Metallothionein (Copper-Inducible)
    Available Since
    Aug. 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    47032-plasmid-map-sequence-id-67069

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  14. pFastBac HT JS-Munc18c

    Plasmid
    #135555
    Purpose
    Express mouse Munc18c in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18c.
    Depositor
    Jingshi Shen
    Article
    Yu et al Methods Mol Biol. 2019;1860:237-249. doi
    Insert
    Munc18c
    Tags
    6xHis tag and TEV site
    Expression
    Insect
    Promoter
    polyhedrin promoter
    Available Since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    135555-plasmid-map-sequence-id-272498

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  16. pYSD061

    Plasmid
    #212915
    Purpose
    Encodes the S. cerevisiae SUC2 (Invertase) protein (minus signal peptide) as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)
    Depositor
    Paul Young
    Article
    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102
    Insert
    SUC2 (SUC2 Budding Yeast)
    Expression
    Bacterial
    Available Since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    212915-plasmid-map-sequence-id-421750

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  19. pOCC65

    Plasmid
    #118865
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal monomeric GFP
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-mGFP-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease, mGFP
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118865-plasmid-map-sequence-id-232317

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  20. ZJOM135

    Plasmid
    #133659
    Purpose
    Integration of SEC63-2mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast endoplasmic reticulum (ER).
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-SEC63-2mTagBFP2 (SEC63 Budding Yeast)
    Tags
    mTagBFP2
    Expression
    Yeast
    Available Since
    Dec. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    133659-plasmid-map-sequence-id-265905

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Showing: 1221 - 1240 of 10980 results