We narrowed to 10,644 results for: cat.1

1. -962 human cyclin D1 promoter AP-1 site mutant pGL3Basic

   Plasmid

   #32728

   Depositor

   Frank McCormick

   Article

   Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

   Insert

   CCND1 promoter (CCND1 Human)
   

   Use

   Luciferase; Luciferase reporter gene

   Tags

   Expression

   Mutation

   -947 AP-1 binding site TGAGTCA deletion

   Promoter

   -962 CCND1 promoter

   Available since

   Jan. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

2. -962 human cyclin D1 promoter TCF (1) site mutant pGL3Basic

   Plasmid

   #32733

   Depositor

   Frank McCormick

   Article

   Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

   Insert

   CCND1 promoter (CCND1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   -75 TCF(1) site CTTTGAT deletion

   Promoter

   -962 CCND1

   Available since

   Jan. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pB80-hsKIF5B(1-560)Rigor-L-mVenus-L-SspB(micro)

   Plasmid

   #193721

   Purpose

   Optogenetic coupling to dimeric human kinesin-1 (KIF5B aa1-807) via SSPB(micro) for anterograde transport

   Depositor

   Lukas Kapitein

   Article

   Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

   Insert

   hsKIF5B(1-560)Rigor-L-mVenus-L-SspB(micro) (KIF5B Human, Synthetic)
   

   Use

   Tags

   mVenus-SSPB(micro)

   Expression

   Mammalian

   Mutation

   hsKIF5B(aa1-560), RIGOR: G234A; mVenus: Met1Del, …

   Promoter

   Chicken beta-actin

   Available since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLL3.7m-Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)

   Plasmid

   #83841

   Purpose

   Fluorescent probe for G1/S transition and M/G1 transition

   Depositor

   Michael Lin

   Article

   Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120) (GMNN, CDT1 Human, Synthetic)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS4134

   Plasmid

   #131747

   Purpose

   Human reduced folate transporter 1 SLC19A1 for lentiviral transduction, copGFP transduction marker

   Depositor

   Christina Smolke

   Article

   Xiang et al Nat Commun. 2019 Sep 23;10(1):4327. doi:

   Insert

   Human reduced folate carrier 1 (SLC19A1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKM402

   Plasmid

   #107770

   Purpose

   Expression of phage Che9c RecT for oligo-mediated recombineering in mycobacteria

   Depositor

   Kenan Murphy

   Article

   Murphy et al Methods Mol Biol. 2015;1285:177-99. doi:

   Inserts

   Che9C RecT
   TetR (tet repressor)
   cat
   kan
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Cat promoter, Ptet, kan promoter, and pTB21

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28b-SUMO-FLAG-TeKaiB-1-94-Y8A-Y94A-FLAG

   Plasmid

   #229223

   Purpose

   For expression of T. elongatus KaiB protein mutant

   Depositor

   Andy LiWang

   Insert

   kaiB (kaiB T. elongatus)
   

   Use

   Tags

   FLAG and SUMO-FLAG

   Expression

   Bacterial

   Mutation

   1-94 (truncated), Y8A, Y94A

   Promoter

   Available since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. Helios-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

   Plasmid

   #75246

   Purpose

   CRISPR/Cas9 NICKASE plasmid against human Helios (1/2)

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against human Helios (IKZF2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSico_U6-Pan-PGC1a sgRNA

   Plasmid

   #165426

   Purpose

   Expression of gRNA against human Total PGC-1a variants

   Depositor

   Antonio Amelio

   Article

   Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

   Insert

   gRNA against human Total PGC-1a variants (PPARGC1A Human, Synthetic)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Afp

    Plasmid

    #99696

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Afp, vector allows for strong activation of mouse Afp, can be packaged and delivered as AAV

    Depositor

    George Church

    Insert

    dCas9 and gRNA targeting Afp (Afp Synthetic)
    

    Use

    AAV

    Tags

    VPR mini

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. SG3ΔENV CA E45A

    Plasmid

    #145783

    Purpose

    Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and E45A mutation in capsid (CA E45A).

    Depositor

    Wesley Sundquist

    Article

    Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/

    Insert

    Capsid
    

    Use

    Lentiviral and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Changed Capsid residue glutamic acid 45 to alanin…

    Promoter

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. SG3ΔENV CA M66I

    Plasmid

    #149687

    Purpose

    Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and M66I mutation in capsid (CA M66I).

    Depositor

    Wesley Sundquist

    Article

    Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/

    Insert

    Capsid
    

    Use

    Lentiviral and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Changed Capsid residue Met 66 to Iso (CA M66I)

    Promoter

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Hbb

    Plasmid

    #99692

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Hbb, vector allows for strong activation of mouse Hbb, can be packaged and delivered as AAV

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9 and gRNA targeting Hbb (Hbb Synthetic)
    

    Use

    AAV

    Tags

    VPR mini

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. p36-p37-p38-p40-pET-Duet-1-Rfc3-T76D

    Plasmid

    #175051

    Purpose

    Overexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coli

    Depositor

    Brian Kelch

    Article

    Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

    Insert

    Replication factor C subunit 5 (RFC2, RFC3, RFC4, RFC5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changed Thr76 to Asp, phosphomimetic mutation

    Promoter

    T7 promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. p36-p37-p38-p40-pET-Duet-1-Rfc3-3KtoA

    Plasmid

    #175050

    Purpose

    Overexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coli

    Depositor

    Brian Kelch

    Article

    Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

    Insert

    Replication factor C subunits 2-5, Rfc3-3KtoA-mutation (RFC4, RFC2, RFC3, RFC5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Neurog2

    Plasmid

    #99694

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9 and gRNA targeting Actc1 (gRNA: GGTATATAAGGGGTTTTAAG) (Actc1 Synthetic, Mouse)
    

    Use

    AAV and CRISPR

    Tags

    VPR mini

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Jan. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFastBac-Dual_BNIP3(1-158aa)-THROMBIN-GST (W18A/L21A; deltaLIR)

    Plasmid

    #223778

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    BNIP3 soluble part (BNIP3 Human)
    

    Use

    Tags

    Thrombin cleavage-GST

    Expression

    Insect

    Mutation

    W18A/L21A; deletion of LIR

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETDuet1_NIX(1-182aa)-EGFP-TEV-GST (W36A/L39A; deltaLIR)

    Plasmid

    #223748

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    NIX (BNIP3L) soluble part (BNIP3L Human)
    

    Use

    Tags

    EGFP-TEV-GST

    Expression

    Bacterial

    Mutation

    W36A/L39A; deletion of LIR

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (I224A/L227A; deltaLIR3)

    Plasmid

    #223775

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    BCL2L13 soluble part (BCL2L13 Human)
    

    Use

    Tags

    EGFP-TEV-GST

    Expression

    Bacterial

    Mutation

    I224A/L227A; deletion of LIR3

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (I307A/V310A; deltaLIR4)

    Plasmid

    #223776

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    BCL2L13 soluble part (BCL2L13 Human)
    

    Use

    Tags

    EGFP-TEV-GST

    Expression

    Bacterial

    Mutation

    I307A/V310A; deletion of LIR4

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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