-
Plasmid#118051PurposeGBPart - Operator/DNA Response element - 4 copies the SMAD DNA binding motifDepositorInsert4 copies the SMAD DNA binding motif
UseSynthetic Biology; Domestication of dna parts for…TagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
FB399
Plasmid#203635PurposeModule for the expression of geneticin resistance, Nluc under PgpdA promoter and luciferase under a synthetic promoter containing two copies of the target sequence for gRNA1 (2xLuc).DepositorInsertFB367+FB396
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEMS1177
Plasmid#29091PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been testedDepositorInsertPle81 (GAL Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-NLSExpressionMutationPromoterAvailable sinceFeb. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
mKeima-Red-Golgi-7
Plasmid#56014PurposeLocalization: GalT Targeting Sequence, Excitation: 440, Emission: 620DepositorInsertGolgi (B4GALT1 Human)
UseTagsmKeima-RedExpressionMammalianMutationaa 1-82 of NM_001497.3PromoterCMVAvailable sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-M45HA
Plasmid#17573DepositorInsertmurine cytomegalovirus M45 gene
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro
Plasmid#213026PurposeExpresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2DepositorInsertHuman LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag
UseTags3xFlag (ORF2p)ExpressionMammalianMutationPromoterCMVAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLD564 human L1 ORF2p-3xFlag (L1RP, CMV promoter) in pCEP4 Puro
Plasmid#213030PurposeExpresses full length human LINE-1 in human cells (native L1RP sequence), with a C-terminal 3xFlag tag on ORF2DepositorInsertHuman LINE-1 (L1RP Sequence) with 5' UTR and ORF2-3xFlag
UseTags3xFlag (ORF2p)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC0.017
Plasmid#119621PurposeLevel 0 Part. CDSDepositorInsertdCas9
UseSynthetic BiologyTagsExpressionMutationbp 331 C to A , 1237 A to C, 1284 C to T, 2121 C …PromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.178
Plasmid#119645PurposeLevel 0 Part. Insertion siteDepositorInsert7942 NS3 Down Flank
UseSynthetic BiologyTagsExpressionMutation4 nucleotide insertion between nt 65 and 66 in NS…PromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.175
Plasmid#119643PurposeLevel 0 Part. Insertion siteDepositorInsert7942 NS2 Up Flank
UseSynthetic BiologyTagsExpressionMutationbp 422 A to T, bp 423 A to T, bp 663 inserted G. …PromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.018
Plasmid#119622PurposeLevel 0 Part. CDSDepositorInsertdCas9+deg-tag
UseSynthetic BiologyTagsExpressionMutationbp 331 C to A , 1237 A to C, 1284 C to T, 2121 C …PromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.055
Plasmid#119566PurposeLevel 0 part. PromoterDepositorInsertPisiAB
UseSynthetic BiologyTagsExpressionMutationbp 391 G to A, RBS* added (22 bp) upstream of ATG…PromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAc-Neo-OVA
Plasmid#22533DepositorInsertOVA (OVAL Chicken)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pT26CfpSox10G4FER3(#1048)
Plasmid#184073Purposecyclofen-inducible GAL4 activation in zebrafish permanent transgenicDepositorInsertGal4bdFF-ERT2
UseZebrafish transgenesis (blue heart)TagsExpressionMutationERT2: G400V, M543A, L544A, deleted 1-281;PromoterZf-Sox10Available sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
PS-CFP-Golgi-7
Plasmid#57204PurposeLocalization: Golgi Complex / GalT, Excitation: 400 / 490, Emission: 468 / 511DepositorInsertGolgi (B4GALT1 Human)
UseTagsPS-CFPExpressionMammalianMutationaa 1-82 of NM_001497.3PromoterCMVAvailable sinceApril 1, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pT22i2Shh24G4FER3(#1041)
Plasmid#184072Purposecyclofen-inducible GAL4 activation in zebrafish permanent transgenicDepositorInsertGal4bdFF-ERT2
UseZebrafish transgenesis (blue eyes)TagsExpressionMutationERT2: G400V, M543A, L544A, deleted 1-281;PromoterZf-Shha2.4Available sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT2iaTubG4FER3(#940)
Plasmid#184071Purposecyclofen-inducible GAL4 activation in zebrafish transient transgenicDepositorInsertGal4bdFF-ERT2
UseZebrafish transient transgenesisTagsExpressionMutationERT2: G400V, M543A, L544A, deleted 1-281;PromoterGfish-a1TubAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
18065-M03-412
Plasmid#225666PurposeLentivirus protein expression. Control plasmid.DepositorInsertsUseLentiviralTags3xFLAGExpressionMutationC-terminally truncated (aa 1-333 only)PromoterWPRE-SV40p and mCMVpAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
18065-M04-412
Plasmid#225667PurposeLentivirus protein expression. Control plasmid.DepositorInsertsUseLentiviralTags3xFLAGExpressionMutationC-terminally truncated (aa 1-333 only)PromoterWPRE-SV40p and mCMVpAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pACT2-μ3A
Plasmid#198174PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-3 μ3A fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-3 μ3A
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationsilent substitution in codon 225PromoterADH1Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only