Skip to main content
Addgene
Showing: 1221 - 1240 of 2456 results
  1. FB399

    Plasmid
    #203635
    Purpose
    Module for the expression of geneticin resistance, Nluc under PgpdA promoter and luciferase under a synthetic promoter containing two copies of the target sequence for gRNA1 (2xLuc).
    Depositor
    Insert
    FB367+FB396
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
  2. mKeima-Red-Golgi-7

    Plasmid
    #56014
    Purpose
    Localization: GalT Targeting Sequence, Excitation: 440, Emission: 620
    Depositor
    Insert
    Golgi (B4GALT1 Human)
    Use
    Tags
    mKeima-Red
    Expression
    Mammalian
    Mutation
    aa 1-82 of NM_001497.3
    Promoter
    CMV
    Available since
    April 28, 2015
    Availability
    Academic Institutions and Nonprofits only
  3. pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro

    Plasmid
    #213026
    Purpose
    Expresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag
    Use
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. pLD564 human L1 ORF2p-3xFlag (L1RP, CMV promoter) in pCEP4 Puro

    Plasmid
    #213030
    Purpose
    Expresses full length human LINE-1 in human cells (native L1RP sequence), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Insert
    Human LINE-1 (L1RP Sequence) with 5' UTR and ORF2-3xFlag
    Use
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. pT26CfpSox10G4FER3(#1048)

    Plasmid
    #184073
    Purpose
    cyclofen-inducible GAL4 activation in zebrafish permanent transgenic
    Depositor
    Insert
    Gal4bdFF-ERT2
    Use
    Zebrafish transgenesis (blue heart)
    Tags
    Expression
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    Zf-Sox10
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. PS-CFP-Golgi-7

    Plasmid
    #57204
    Purpose
    Localization: Golgi Complex / GalT, Excitation: 400 / 490, Emission: 468 / 511
    Depositor
    Insert
    Golgi (B4GALT1 Human)
    Use
    Tags
    PS-CFP
    Expression
    Mammalian
    Mutation
    aa 1-82 of NM_001497.3
    Promoter
    CMV
    Available since
    April 1, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
  7. pT22i2Shh24G4FER3(#1041)

    Plasmid
    #184072
    Purpose
    cyclofen-inducible GAL4 activation in zebrafish permanent transgenic
    Depositor
    Insert
    Gal4bdFF-ERT2
    Use
    Zebrafish transgenesis (blue eyes)
    Tags
    Expression
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    Zf-Shha2.4
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  8. pT2iaTubG4FER3(#940)

    Plasmid
    #184071
    Purpose
    cyclofen-inducible GAL4 activation in zebrafish transient transgenic
    Depositor
    Insert
    Gal4bdFF-ERT2
    Use
    Zebrafish transient transgenesis
    Tags
    Expression
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    Gfish-a1Tub
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
  9. pACT2-μ3A

    Plasmid
    #198174
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-3 μ3A fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Insert
    AP-3 μ3A
    Use
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    silent substitution in codon 225
    Promoter
    ADH1
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 1221 - 1240 of 2456 results