165,817 results

1. LZRS-DNMT1

   Plasmid

   #24952

   Depositor

   Paul Khavari

   Article

   Sen et al Nature. 2010 Jan 28. 463(7280):563-7.

   Insert

   DNMT1 (DNMT1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. B52 (empty plasmid backbone to express 2 sgRNAs)

   Plasmid

   #100708

   Purpose

   Empty plasmid backbone to express 2 sgRNAs (use BbsI and BsmBI for cloning)

   Depositor

   Alberto Ciccia

   Article

   Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoters

   Available Since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSUMO3

   Plasmid

   #52260

   Purpose

   Bacterial expression of human 6His-tagged SUMO3, SUMO-E1 and SUMO-E2

   Depositor

   Primo Schaer

   Article

   Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

   Inserts

   SUMO-3 (SUMO3 Human)
   UBE2l (UBE2I Human)
   SAE-2 (UBA2 Human)
   SAE-1 (SAE1 Human)
   

   Use

   Tags

   6His-tag

   Expression

   Bacterial

   Mutation

   C-terminal gly-gly

   Promoter

   T7 and rbs

   Available Since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRBC-sfGFP-150TAG

   Plasmid

   #174076

   Purpose

   Plasmid with p15a origin of replication expressing codon optimized superfolder GFP-150 TAG with C-terminal His6 tag, under T7 promoter,

   Depositor

   Ryan Mehl

   Article

   Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

   Insert

   Superfolder GFP
   

   Use

   Tags

   His-6

   Expression

   Bacterial

   Mutation

   N150TAG

   Promoter

   T7

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLHCX-FLAG-mPKM2

   Plasmid

   #44239

   Depositor

   Lewis Cantley, Matthew Vander Heiden

   Article

   Christofk et al Nature. 2008 Mar 13;452(7184):230-3. doi

   Insert

   Pyruvate Kinase M2 (Pkm Mouse)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 29, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pX330-PITCh-GOLGA2

   Plasmid

   #207791

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of GOLGA2 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of GOLGA2 (GOLGA2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Dec. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPRE

   Plasmid

   #122151

   Purpose

   Expresses dCas9-SunTag(24X) fusion and a fluorescent indicator BFP.

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

   Insert

   dCas9 fused to SunTag(24x)
   

   Use

   Lentiviral

   Tags

   2xNLS-P2A-BFP and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available Since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pN1/G3BP1-iRFP

   Plasmid

   #129339

   Purpose

   Stress granule marker

   Depositor

   Yasushi Okada

   Article

   Yaginuma et al bioRxiv 2021.10.08.463131

   Insert

   iRFP (from Dr. M. Matsuda)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLGB13

   Plasmid

   #126618

   Purpose

   Suicide vector for allelic replacement in Bacteroides, erythromycin selection and aTC-inducible ss-Bfe1 counterselection

   Depositor

   Laurie Comstock

   Article

   Garcia-Bayona et al mBio. 2019 Aug 13;10(4). pii: mBio.01762

   Type

   Empty backbone

   Use

   Allellic exchange in bacteria

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLS-mP

    Plasmid

    #81225

    Purpose

    Lentivirus enhancer assay plasmid that contains a minimal promoter (mP) and a EGFP reporter gene.

    Depositor

    Nadav Ahituv

    Article

    Inoue et al Genome Res. 2016 Nov 9. pii: gr.212092.1

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Derived from pGL4.23

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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