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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,915 results for: SPI

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Showing: 1241 - 1260 of 5915 results

  1. pHYP-NPC-10H

    Plasmid
    #162789
    Purpose
    SARS-CoV-2 Nucleocapsid Protein expression in E.coli
    Depositor
    Ivan Vorobiev
    Article
    Kolesov et al PeerJ. 2022 Jan 7;10:e12751. doi: 10.771
    Insert
    Nucleocapsid phosphoprotein [Severe acute respiratory syndrome coronavirus 2] (N Severe acute respiratory syndrome coronavirus 2)
    Tags
    10xHis
    Expression
    Bacterial
    Promoter
    T7lac
    Available Since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    162789-plasmid-map-sequence-id-333793

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  5. pGBW-m4046810

    Plasmid
    #149251
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 N (nucleocapsid)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 N (nucleocapsid) (N Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;HA
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    May 11, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149251-plasmid-map-sequence-id-357431

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  10. pPRISM-QF2-he1.1:YFP

    Plasmid
    #184819
    Purpose
    Modified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Backbone into which homology arms are cloned for CRISPR/Cas9-mediated integration of QF2.
    Depositor
    Marnie Halpern
    Article
    Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7
    Insert
    QF2-pA-he1.1:YFP
    Use
    CRISPR; Backbone for cloning of homology arms (fo…
    Available Since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    184819-plasmid-map-sequence-id-366121

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  11. pPRISM-QF2-he1.1:YFP-rln3a-HA

    Plasmid
    #184820
    Purpose
    Modified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Contains homology arm inserts for integration of QF2 into rln3a gene by CRISPR/Cas9 integration.
    Depositor
    Marnie Halpern
    Article
    Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7
    Insert
    rln3a-HA
    Use
    CRISPR; For integration of qf2 by homology-direct…
    Available Since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    184820-plasmid-map-sequence-id-366119

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  16. pET24a-LaM4-1xTS

    Plasmid
    #162778
    Purpose
    Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to one tyrosine sulfation (TS) motif. LaM4-1xTS also contains a T7, HA, BAP and His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii
    Insert
    anti-mCherry nanobody fused to a TS site, T7, HA, BAP and His6 epitope
    Tags
    BAP, HA, His6, T7, and TS site from proCCK
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    162778-plasmid-map-sequence-id-321887

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Showing: 1241 - 1260 of 5915 results