We narrowed to 5,457 results for: aaas

1. pENTRY4_ Pi21

   Plasmid

   #126904

   Purpose

   Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.

   Depositor

   Sophien Kamoun

   Insert

   Wheat_live_Cas9
   

   Use

   CRISPR

   Available Since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICSL4723_ Pi21

   Plasmid

   #126892

   Purpose

   Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.

   Depositor

   Sophien Kamoun

   Insert

   Wheat_live_Cas9
   

   Use

   CRISPR

   Available Since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-KrasWT/sgKras/Cre

   Plasmid

   #99848

   Purpose

   Expresses Cre-recombinase, a barcoded HDR template that serves as a control for HDR into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

   Depositor

   Monte Winslow

   Article

   Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

   Insert

   Kras (Kras Mouse)
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Mutation

   AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

   Available Since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-KrasG13C/sgKras/Cre

   Plasmid

   #99856

   Purpose

   Expresses Cre-recombinase, a barcoded HDR template to introduce a G13C mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

   Depositor

   Monte Winslow

   Article

   Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

   Insert

   Kras (Kras Mouse)
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Mutation

   AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

   Available Since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-KrasG12R/sgKras/Cre

   Plasmid

   #99852

   Purpose

   Expresses Cre-recombinase, a barcoded HDR template to introduce a G12R mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

   Depositor

   Monte Winslow

   Article

   Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

   Insert

   Kras (Kras Mouse)
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Mutation

   AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

   Available Since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-KrasG13D/sgKras/Cre

   Plasmid

   #99857

   Purpose

   Expresses Cre-recombinase, a barcoded HDR template to introduce a G13D mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

   Depositor

   Monte Winslow

   Article

   Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

   Insert

   Kras (Kras Mouse)
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Mutation

   AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

   Available Since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-KrasG12S/sgKras/Cre

   Plasmid

   #99853

   Purpose

   Expresses Cre-recombinase, a barcoded HDR template to introduce a G12S mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

   Depositor

   Monte Winslow

   Article

   Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

   Insert

   Kras (Kras Mouse)
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Mutation

   AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

   Available Since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAV-KrasG12A/sgKras/Cre

   Plasmid

   #99849

   Purpose

   Expresses Cre-recombinase, a barcoded HDR template to introduce a G12A mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

   Depositor

   Monte Winslow

   Article

   Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

   Insert

   Kras (Kras Mouse)
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Mutation

   AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

   Available Since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. MB 98w3 ttrSR(m13)-Bxb1_P7-bARGSer

   Plasmid

   #232473

   Purpose

   Optimized tetrathionate sensor with recombinase switch and acoustic reporter genes (bARGSer)

   Depositor

   Mikhail Shapiro

   Article

   Buss et al Nat Commun. 2025 Aug 25;16(1):7931. doi:

   Inserts

   ttrS
   ttrR
   PttrB185-269
   bARGSer
   AxeTxe
   Bxb1 integrase
   

   Tags

   ssrA degradation tag

   Expression

   Bacterial

   Mutation

   the gene Ser39006_001280 was deleted from the clu…

   Promoter

   Constitutive (TTGATAGCTAGCTCAGTCCTAGGTATTGTGCTAGC…

   Available Since

   Sept. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEX-A-U6-MaSgRNA_PuroR

    Plasmid

    #84780

    Purpose

    Expresses major satellite-specific sgRNA.

    Depositor

    Heinrich Leonhardt

    Article

    Schmidtmann et al Nucleus. 2016 Sep 27:0.

    Insert

    major satellite-specific sgRNA
    

    Use

    CRISPR

    Expression

    Bacterial and Mammalian

    Promoter

    U6

    Available Since

    July 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. MB 93C1 thsS(t3)R-Bxb1_P7-bARGSer

    Plasmid

    #232469

    Purpose

    Optimized thiosulfate sensor with recombinase switch and acoustic reporter genes (bARGSer)

    Depositor

    Mikhail Shapiro

    Article

    Buss et al Nat Commun. 2025 Aug 25;16(1):7931. doi:

    Inserts

    thsS(t3)
    thsR
    PphsA342
    bARGSer
    AxeTxe
    Bxb1 integrase
    

    Tags

    ssrA degradation tag

    Expression

    Bacterial

    Mutation

    Contains the following mutations K286Q, Q350H, I…

    Promoter

    J23104 (ttgacagctagctcagtcctaggtattgtgctagc), J23…

    Available Since

    Sept. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL4-eIF3B MBE2 mutant

    Plasmid

    #133309

    Purpose

    Expression of luciferase driven by eIF3B promoter region mutated at the E-box binding motif 2

    Depositor

    Josep Domingo-Domenech

    Article

    Santasusagna et al Cancer Discov. 2023 Sep 7. doi: 10.1158/

    Insert

    eIF3B promoter MBE2 mutant (EIF3B Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    gccacatgcacc changed to gcAaAaAAcacc

    Promoter

    eIF3B

    Available Since

    Oct. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. PLKO.1-STAU1-CDS

    Plasmid

    #136048

    Purpose

    STAU1 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (CGAGTAAAGCCTAGAATCAAA)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    STAU1 (STAU1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLH-stsgRNA3.1

    Plasmid

    #64118

    Purpose

    Vector for expression of St1 sgRNA3.1 in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    St1 sgRNA3.1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330-NEAT1pr_v1

    Plasmid

    #97081

    Purpose

    Encodes an sgRNA that creates a DSB at the promoter of human NEAT1 gene

    Depositor

    Archa Fox

    Article

    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

    Insert

    sgRNA NEAT1pr_v1
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSDMA66

    Plasmid

    #128355

    Purpose

    gRNA ribozyme construct with Cryptococcus neoformans ACT1 promter and TRP1 terminator. Following ribozyme cleavage, a gRNA targeting ADE2 is liberated.

    Depositor

    James Fraser

    Article

    Arras et al PLoS One. 2016 Oct 6;11(10):e0164322. do

    Insert

    Noureothricin (NAT)
    

    Use

    Unspecified

    Promoter

    ACT1

    Available Since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSDMA67

    Plasmid

    #128356

    Purpose

    gRNA ribozyme construct with Cryptococcus neoformans ACT1 promter and TRP1 terminator. Following ribozyme cleavage, a gRNA targeting ADE2 is liberated.

    Depositor

    James Fraser

    Article

    Arras et al PLoS One. 2016 Oct 6;11(10):e0164322. do

    Insert

    Noureothricin (NAT)
    

    Use

    Unspecified

    Promoter

    ACT1

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PLKO.1-UPF3A-3'UTR

    Plasmid

    #136042

    Purpose

    UPF3A shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (GACGTAGAAACACGCAGAAAC)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    UPF3A (UPF3A Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAW218

    Plasmid

    #113240

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader IHF binding site flip

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Expression

    Bacterial

    Mutation

    AAAAAATCATTAATTAATAATAGGTTATG->CATAACCTATTATTA…

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. FUGW-APPSwe-T2A-mCherry

    Plasmid

    #190804

    Purpose

    Express Swedish mutant APP from Ubiquitin C promoter, co-expressing mCherry

    Depositor

    Ronald Hart

    Article

    Ma et al J Neurosci. 2023 Mar 8;43(10):1830-1844.

    Insert

    APP (APP Human)
    

    Use

    Lentiviral

    Mutation

    Swedish (KM670/671NL)

    Promoter

    Ubi C

    Available Since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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