We narrowed to 6,231 results for: nls

1. pcDNA3.2/NS5-YFP-NLS-mut

   Plasmid

   #120832

   Purpose

   Ectopic expression of ZIKV protein

   Depositor

   Jan Rehwinkel

   Article

   Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

   Insert

   ZIKV NS5-eYFP "NLS-mut"
   

   Use

   Tags

   eYFP-V5

   Expression

   Mammalian

   Mutation

   R372A/Q373A/K387A/H388A/K389A

   Promoter

   CMV

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLIK 3XFLAG-EGFP-NLS hygro

   Plasmid

   #87781

   Purpose

   Inducible expression of EGFP with a C-terminal NLS sequence

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   eGFP
   

   Use

   Lentiviral

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. ZIF-NLS-EGFP(Y66F)-HOTag6

   Plasmid

   #208749

   Purpose

   part B of nls-SparkDrop with non-fluorescent eGFP

   Depositor

   Xiaokun Shu

   Article

   Chung et al ACS Cent Sci. 2023 Jul 7;9(7):1466-1479.

   Insert

   ZIF-NLS-EGFP(Y66F)-HOTag6
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)-myc-OGA(GS-544)C181_NLS

   Plasmid

   #193997

   Purpose

   plasmid encoding myc-tagged OGA(GS-544)C181_NLS

   Depositor

   Christina Woo

   Article

   Ge et al ACS Chem Biol. 2023 Jan 4. doi: 10.1021/

   Insert

   myc-OGA(GS-544)C181_NLS
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-MED1trunc-IDR-YFP-NLS

   Plasmid

   #145275

   Purpose

   Mammalian Expression of Nuclear MED1 truncated IDR-YFP with SV40 NLS

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   MED1-IDR truncated (MED1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. SIV URA PCP-NLS-GFPEnvy

   Plasmid

   #189941

   Purpose

   Single-integration vector for inserting PCP-NLS-GFPEnvy at the URA3 locus in Saccharomyces cerevisiae

   Depositor

   Tineke Lenstra

   Article

   Brouwer et al Nat Struct Mol Biol. 2023 May;30(5):692-

   Insert

   PCP-NLS-GFPEnvy
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-Synapsin-miRFP670-3XNLS-WPRE

   Plasmid

   #159952

   Purpose

   nucleus marker

   Depositor

   Michael Courtney

   Article

   Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

   Insert

   miRFP670-3XNLS
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   synthetic

   Promoter

   hSyn1

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNTKV- Elf5-KI-3'UTR-2A-EYFP-NLS

   Plasmid

   #128833

   Purpose

   Knock-in of nuclear EYFP into mouse Elf5 locus

   Depositor

   Yossi Buganim

   Article

   Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

   Insert

   Nuclear EYFP into Elf5 locus (Elf5 Mouse)
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-T7-AcrIIA17_Efa-NLS(SV40) (KAC91)

   Plasmid

   #134333

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA17_Efa with C-terminal NLS (SV40)

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized AcrIIA17_Efa
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. 3xnls-cpmTq2-Calcium-lifetime-sensor

    Plasmid

    #129626

    Purpose

    Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

    Depositor

    Dorus Gadella

    Article

    van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

    Insert

    3xnls-cpmTq2-Calcium-lifetime-sensor
    

    Use

    Tags

    3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pC4EN-F1-mGAF709 (NLS-FKBP-mGAF709)

    Plasmid

    #80004

    Purpose

    miRFP709 GAF domain fused to FKBP and NLS

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Insert

    FKBP
    

    Use

    Tags

    GAF domain of miRFP709 and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCA15-Ubc-NLS- scFV-tdTomato

    Plasmid

    #199446

    Purpose

    Expression of scFV-tdTomato that binds to the GCN4 peptide from the SunTag System in mammalian cells

    Depositor

    Baohui Chen

    Article

    Xu et al J Virol. 2022 Dec 21;96(24):e0092022. do

    Insert

    scFV-tdTomato
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human ubiquitin C (UBC)

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAG413 GAL ORF1 Shuffle1 NLS

    Plasmid

    #145794

    Purpose

    mPing Yeast Transposition Assay

    Depositor

    Charles Nathan Hancock

    Article

    Payero et al Journal of the South Carolina Academy of

    Insert

    ORF1 Shuffle1 NLS
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Enhanced Nucleur Localization Signal

    Promoter

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEMHE-NLS-Beta-catenin-S33Y

    Plasmid

    #138537

    Purpose

    For making Beta-catenin-S33Y RNA

    Depositor

    Carmen Williams

    Article

    Gambini et al Dev Cell. 2020 Jun 8;53(5):545-560.e7. d

    Insert

    Beta-catenin
    

    Use

    In vitro transcription

    Tags

    2XFLAG and 2xNLS

    Expression

    Mutation

    changed serine 33 to tyrosine

    Promoter

    T7

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEJS1004-pCSDest2-AcrIIA5-NLS-FLAG

    Plasmid

    #136513

    Purpose

    Expresses type II-A anti-CRISPR protein AcrIIA5 in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Garcia et al Cell Rep. 2019 Nov 12;29(7):1739-1746.e5

    Insert

    codon-optimized AcrIIA5
    

    Use

    Tags

    FLAG/NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. FokI(inactive)-mCherry-LacI-NLS

    Plasmid

    #215100

    Purpose

    Expresses FokI(inactive)-mCherry-LacI-NLS in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    FokI(inactive)-mCherry-LacI-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    FokI(inactive) mutant D69A.

    Promoter

    CMV

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. EYFP-NLS-actin-K68/284R

    Plasmid

    #60616

    Purpose

    Encodes YFP and NLS tagged beta-actin with sumoylation resistant mutations

    Depositor

    Primal de Lanerolle

    Article

    Hofmann et al J Cell Biol. 2009 Jul 27;186(2):193-200.

    Insert

    Beta-actin (ACTB Human)
    

    Use

    Tags

    EYFP and NLS

    Expression

    Mammalian

    Mutation

    K68R, 284R

    Promoter

    Available Since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pT2ADW-lox-mCherryNLS-pA-lox-4493NES

    Plasmid

    #138374

    Purpose

    Booster-PKA for the generation of mice

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

    Insert

    lox-mCherry-lox-Booster-PKA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIRES-mRFP-NLS-PCNA (2514)

    Plasmid

    #61850

    Purpose

    cell cycle marker

    Depositor

    Jonathon Pines

    Insert

    PCNA (PCNA Human)
    

    Use

    Tags

    NLS and mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

20. A3Ai E72A-Cas9n-UGI-NLS

    Plasmid

    #109430

    Purpose

    Expresses catalytically inactive human APOBEC3A with an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.

    Depositor

    Reuben Harris

    Article

    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

    Insert

    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A E72A Catalytic Mutant (APOBEC3A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Insertion of an L1 intron into the coding sequenc…

    Promoter

    CMV

    Available Since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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