We narrowed to 1,335 results for: piggyBac

1. pPB-tetO(hCMV1)-HA-Tet1mHxD(201R2)-IV

   Plasmid

   #102422

   Purpose

   Inducible expression of siRNA resistant mouse Tet1-201 (ENSMUST00000050826.13) with mutated catalytic domain (H1620Y & D1622A), HA-tag and IRES-Venus in piggyBac (PB) transposon vector

   Depositor

   Kian Peng Koh

   Article

   Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi

   Insert

   Tet1 catalytic domain mutant (Tet1 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   H1620Y and D1622A mutations in Tet1 catalytic dom…

   Promoter

   TetO-CMV

   Available Since

   Nov. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pB-Tet-On-Arl13b-RhoA sensor

   Plasmid

   #189613

   Purpose

   Tet-On cilia-targeted RhoA sensor (Xlone piggybac back-bone); Arl13b-fused with a sGFP-mScarlet-I based sensor

   Depositor

   David Clapham

   Article

   Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

   Insert

   Arl13b
   

   Use

   Tags

   GFP and mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   Tet3G

   Available Since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-CuO-V5 CASC3 siRes 1-480 WT

   Plasmid

   #158541

   Purpose

   For PiggyBac-mediated integration of V5-tagged siRNA-resistant CASC3 1-480

   Depositor

   Niels Gehring

   Article

   Gerbracht et al Nucleic Acids Res. 2020 Jul 4. pii: 5867

   Insert

   CASC3; BTZ; MLN51 (CASC3 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Amino acids 1-480, C-terminal deletion

   Promoter

   CMV

   Available Since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. XLone-Puro beta-catenin P2A eGFP-NLS

   Plasmid

   #155189

   Purpose

   PiggybacTransposon-based tunable and temporal expression control of mutated beta-catenin and nuclear eGFP

   Depositor

   Xiaoping Bao

   Article

   Chang et al Biomaterials. 2022 May 6;285:121569. doi

   Insert

   beta-catenin (Ctnnb1 Mouse)
   

   Use

   Unspecified; Piggybac transposon

   Tags

   P2A eGFP NLS

   Expression

   Mammalian

   Mutation

   Sites phosphorylated by GSK-3beta have been mutat…

   Promoter

   TRE3G

   Available Since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKrox24(MapERK)d1EGFP (KroxDs)

   Plasmid

   #214912

   Purpose

   for PiggyBac mediated integration and stable expression of destabilised EGFP as a reporter to MAPK/ERK pathway activation

   Depositor

   Pavel Krejčí

   Article

   Zlinska et al Mol Ther Nucleic Acids. 2024 Nov 28;36(1

   Insert

   d1EGFP under the MapERK promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MapkERK promoter

   Available Since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB-cT3G-cERP2-HOPX

   Plasmid

   #192923

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of HOPX

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   HOPX (HOPX Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPBCAG-rtTAM2-2A-SOX17-GR-IH

   Plasmid

   #165079

   Purpose

   Ubiquitously expresses rtTAM2, dexamethasone-inducible human SOX17, and hygromycin-resistance gene.

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al Stem Cell Reports. 2021 Mar 23. pii: S22

   Inserts

   rtTA-M2
   SOX17 (SOX17 Human)
   

   Use

   Piggybac transposition

   Tags

   Glucocorticoid Receptor

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB-TRE-V5-TurboID-T2A-SARS-CoV2-NSP7-bGH polyA > mPGK-BlastR-SV40 polyA

   Plasmid

   #218914

   Purpose

   PiggyBac transposition

   Depositor

   Sven Eyckerman

   Article

   Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Insert

   V5-TurboID-T2A-SARS-CoV2-NSP7/BlastR (ORF1ab )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   2363G>C and 4330T>C

   Promoter

   Available Since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB-cT3G-cERP2-KLF6

   Plasmid

   #192909

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of KLF6

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   KLF6 (KLF6 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-CuO-V5 CASC3 siRes 1-480 188/218-mut

    Plasmid

    #158542

    Purpose

    For PiggyBac-mediated integration of V5-tagged siRNA-resistant EJC-binding deficient CASC3 1-480

    Depositor

    Niels Gehring

    Article

    Gerbracht et al Nucleic Acids Res. 2020 Jul 4. pii: 5867

    Insert

    CASC3; BTZ; MLN51 (CASC3 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Amino acids 1-480, C-terminal deletion, F188D, W2…

    Promoter

    CMV

    Available Since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCBh_3x Flag-NLS-OsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid

    #197024

    Purpose

    Vector encoding a human codon-optimized OsCas12f1 driven by CBh promoter, guide RNAs compatible with OsCas12f1 driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Xiangfeng Kong

    Article

    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

    Insert

    humanized OsCas12f1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh, CMV, hU6

    Available Since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-CuO-V5 CASC3 siRes 110-480 WT

    Plasmid

    #158543

    Purpose

    For PiggyBac-mediated integration of V5-tagged siRNA-resistant CASC3 110-480

    Depositor

    Niels Gehring

    Article

    Gerbracht et al Nucleic Acids Res. 2020 Jul 4. pii: 5867

    Insert

    CASC3; BTZ; MLN51 (CASC3 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Amino acids 110-480, C-terminal deletion

    Promoter

    CMV

    Available Since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPB-cT3G-cERP2-KLF4

    Plasmid

    #192908

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of KLF4

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    KLF4 (KLF4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPB-cT3G-cERP2-JUN

    Plasmid

    #192895

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of JUN

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    JUN (JUN Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCBh_3x Flag-NLS-RhCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid

    #197025

    Purpose

    Vector encoding a human codon-optimized RhCas12f1 driven by CBh promoter, guide RNAs compatible with RhCas12f1 driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Xiangfeng Kong

    Article

    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

    Insert

    humanized RhCas12f1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh, CMV, hU6

    Available Since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPB-TRE-V5-TurboID-MUTT2A-SARS-CoV2-NSP7-bGH polyA > mPGK-BlastR-SV40 polyA

    Plasmid

    #218915

    Purpose

    PiggyBac transposition

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    V5-TurboID-MUTT2A-SARS-CoV2-NSP7/BlastR (ORF1ab )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    2363G>C and 4330T>C

    Promoter

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPB-TRE-V5-TurboID-MUTT2A-SARS-CoV2-NCAP-bGH polyA > mPGK-BlastR-SV40 polyA

    Plasmid

    #218913

    Purpose

    PiggyBac transposition

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    V5-TurboID-MUTT2A-SARS-CoV2-NCAP/BlastR (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    2363G>C and 5335T>C

    Promoter

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPB-TRE-V5-TurboID-T2A-SARS-CoV2-NCAP-bGH polyA > mPGK-BlastR-SV40 polyA

    Plasmid

    #218912

    Purpose

    PiggyBac transposition

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    V5-TurboID-T2A-SARS-CoV2-NCAP/BlastR (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    2363G>C and 5335T>C

    Promoter

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPB-TRE-V5-TurboID-linker-MUTT2A-GR-bGH polyA > mPGK-BlastR-SV40 polyA

    Plasmid

    #218911

    Purpose

    PiggyBac transposition

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    V5-TurboID-MUTT2A-huGR/BlastR (NR3C1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    2363G>C and 4155C>T and 4729T>G and 4731…

    Promoter

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNM1325

    Plasmid

    #197144

    Purpose

    CAGp-Target-AID-2A-BlastR for PiggyBac genomic integration

    Depositor

    Nozomu Yachie

    Article

    Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

    Insert

    SpCas9-PmCDA1-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    SpCas9(D10A)

    Promoter

    CAG

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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