We narrowed to 1,383 results for: piggyBac

1. pSLPB3B-mAID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1

   Plasmid

   #187963

   Purpose

   mAID degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance, T2A site and osTIR1 under PGK promoter.

   Depositor

   Edda Schulz

   Article

   Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

   Inserts

   AID-SpdCas9-tagRFPt-P2A-tagBFP; Blasticidin-T2A-osTIR1
   osTIR1
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GGS linker and T2A

   Expression

   Mammalian

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. XLone-Puro Cas13d P2A eGFP-NLS

   Plasmid

   #155182

   Purpose

   PiggybacTransposon-based tunable and temporal expression control of Cas13d and nuclear eGFP

   Depositor

   Xiaoping Bao

   Article

   Chang et al Biomaterials. 2022 May 6;285:121569. doi

   Insert

   CasRx
   

   Use

   Unspecified; Piggybac transposon

   Tags

   NLS and P2A eGFP NLS

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB-cT3G-cERP2-EMX2

   Plasmid

   #192898

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of EMX2

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   EMX2 (EMX2 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPB-cT3G-cERP2-FOSB

   Plasmid

   #192901

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of FOSB

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   FOSB (FOSB Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pW210-pPB-GA-inducible-CRISPRa-sa_dCas9-VPR-hygR

   Plasmid

   #170808

   Purpose

   PiggyBac vector to express GA-inducible VPR-Sa_dCas9 with hygromycin resistance

   Depositor

   Kenneth Yamada

   Article

   Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

   Inserts

   GID1-VPR-IRES-HygR
   GAI-tagBFP-Sa_dCas9 (GAI Synthetic, S. aureus, Mustard Weed)
   

   Expression

   Mammalian

   Mutation

   Synonymous mutation in PYL1 to remove XhoI site (…

   Available Since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEF1a_HH-HEK3 spacer-gRNA scaffold-EC73-5'-donor template-EC73-3'-HDV_pA_pCMV_EGFP-L138S-P2A-mCherry_pA

   Plasmid

   #176460

   Purpose

   Vector for expressing retron Ec73ncRNA-gRNA chimeric RNA (rgRNA) targeting HEK3 site driven by EF1alpha promoter and EGFP(L138S)-P2A-mCherry driven by CMV

   Depositor

   Hui Yang

   Article

   Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

   Insert

   HH-HEK3 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. FLAG-FullLength-TET1-CXXC/LLAA

   Plasmid

   #124399

   Purpose

   Full-length mutant TET1 (L897A/L900A), missing zinc finger domain (585-624), used to PiggyBAC rescue the function of TET1/2 DKO cells

   Depositor

   Shaun Cowley

   Article

   Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

   Insert

   FLAG-FullLength-TET1 CXXC zinc finger deletion (585-624) and L897A/L900A mutations (TET1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   CXXC zinc finger deletion (aa 585-624) and L897A/…

   Available Since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB-cT3G-cERP2-ZFPM2

   Plasmid

   #192922

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of ZFPM2

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   ZFPM2 (ZFPM2 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB-CAG-IB-H2B-HaloTag

   Plasmid

   #247345

   Purpose

   Expresses H2B-Halo under CAG promoter and can be randomly integrated by PiggyBac transposase

   Depositor

   Kazuhiro Maeshima

   Article

   Nagashima et al J Cell Biol. 2019 Mar 1. pii: jcb.201811

   Insert

   H2B (H2BC11) (H2BC11 Human)
   

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Nov. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPB-EF1α-H2B-PA-mCherry-PGKneo

    Plasmid

    #247340

    Purpose

    Expresses photoactivatable H2B-Cherry under EF1 promoter and can be randomly integrated by PiggyBac transposase

    Depositor

    Kazuhiro Maeshima

    Article

    Maeshima et al Curr Biol. 2018 Feb 5;28(3):444-451.e6.

    Insert

    H2B (H2BC11) (H2BC11 Human)
    

    Tags

    PAmCherry

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    Nov. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-EF1-H4-HaloTag-IRES-Neo

    Plasmid

    #247450

    Purpose

    Expresses wild-type H4-Halo under EF1 promoter and can be randomly integrated by PiggyBac transposase

    Depositor

    Kazuhiro Maeshima

    Article

    Semeigazin et al Histochem Cell Biol. 2024 Jul;162(1-2):2

    Insert

    H4 (H4C1) (H4C1 Human)
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    Nov. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPB-CAG-IB-H2B-PA-mCherry

    Plasmid

    #247339

    Purpose

    Expresses photoactivatable H2B-Cherry under CAG promoter and can be randomly integrated by PiggyBac transposase

    Depositor

    Kazuhiro Maeshima

    Article

    Nozaki et al Mol Cell. 2017 Jul 20;67(2):282-293.e7.

    Insert

    H2B (H2BC11) (H2BC11 Human)
    

    Tags

    PAmCherry

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB-PE SpG_ATH51

    Plasmid

    #242072

    Purpose

    Expresses SpG_ATH51 prime editor and pegRNA in mammalian cells

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

    Insert

    SpG_ATH51_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    bpSV40 NLS

    Expression

    Mammalian

    Mutation

    ectopic insertion of 7 amino acids of PID turn-he…

    Available Since

    Oct. 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB_rtTA_BsmB-HNRNPK_sgRNA_4

    Plasmid

    #242899

    Purpose

    PiggyBac cargo vector with HNRNPK sgRNA 4 for dox-inducible knockdown

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al Mol Cell. 2019 Aug 8;75(3):523-537.e10.

    Insert

    HNRNPK (Hnrnpk Mouse)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Sept. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pB-TetOn-DEST-EFS-mODC-rtTA-IRES-NEO (JDW 931)

    Plasmid

    #242578

    Purpose

    PiggyBac transposon flanked, Tet-on, gateway destination vector.

    Depositor

    Joshua Wythe

    Article

    Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

    Insert

    attR1-CmR-ccdB-attR2
    

    Expression

    Mammalian

    Available Since

    Sept. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB_rtTA_BsmB-HNRNPK_sgRNA_1

    Plasmid

    #242897

    Purpose

    PiggyBac cargo vector with HNRNPK sgRNA 1 for dox-inducible knockdown

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al Mol Cell. 2019 Aug 8;75(3):523-537.e10.

    Insert

    HNRNPK (Hnrnpk Mouse)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB_rtTA_BsmB-HNRNPK_sgRNA_3

    Plasmid

    #242898

    Purpose

    PiggyBac cargo vector with HNRNPK sgRNA 3 for dox-inducible knockdown

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al Mol Cell. 2019 Aug 8;75(3):523-537.e10.

    Insert

    HNRNPK (Hnrnpk Mouse)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-PE iSM_PPL2

    Plasmid

    #235996

    Purpose

    Expresses iSM_PPL2 prime editor and pegRNA in mammalian cells

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

    Insert

    iSM_PPL2_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    bpSV40 NLS

    Expression

    Mammalian

    Mutation

    replacement of 5 amino acids of Smac-Cas9 PL2 wit…

    Available Since

    Aug. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPB.DEST-ITGB1(YYFF)-mRuby2

    Plasmid

    #215449

    Purpose

    Piggybac expression vector with integrin beta1 NPxY mutant (YYFF) tagged with mRuby2

    Depositor

    Johanna Ivaska

    Article

    Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

    Insert

    ITGB1 (ITGB1 Human)
    

    Use

    Transposon-based stable expression

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Mutations in the NPxY sites Y783F, Y795F; Silent …

    Promoter

    CAG

    Available Since

    May 29, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pB-Tet-Off_FLEX_Luc-P2A-H2A-mCherry (JDW 970)

    Plasmid

    #229824

    Purpose

    A PiggyBac vector with a cre-dependent dual luciferase / nuclear mCherry reporter. In the presence of cre, this tet-off vector will be ubiquitously expressed in the absence of dox/tet

    Depositor

    Joshua Wythe

    Article

    Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

    Insert

    Luciferase-P2A-H2A-mCherry
    

    Expression

    Mammalian

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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