165,821 results

1. pT3-EF1A-MYC-IRES-luc

   Plasmid

   #129775

   Purpose

   Transposon based vector that expresses human MYC followed by IRES and firefly luciferase

   Depositor

   Amaia Lujambio

   Article

   Ruiz de Galarreta et al Cancer Discov. 2019 Jun 11. pii: 2159-82

   Insert

   human MYC (MYC Human)
   

   Use

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available Since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET11a_3xFlag-nsp14 (SARS-CoV-2)

   Plasmid

   #169159

   Purpose

   To express SARS-CoV-2 nsp14 in E. coli

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

   Insert

   3xFlag-nsp5CS[VATLQ]-nsp14 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   3xFlag and nsp5CS[VATLQ]: predicted nsp5 protease…

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T7

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmCherry-C1_SEPT9 i1

   Plasmid

   #71622

   Purpose

   mammalian expression of human SEPT9 with an mCherry fusion

   Depositor

   Elias Spiliotis

   Article

   Dolat et al J Cell Biol. 2014 Oct 27;207(2):225-35.

   Insert

   Septin 9 (SEPTIN9 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENN.AAV.hSyn.Cre.hGH (AAV9)

   Viral Prep

   #105555-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pENN.AAV.hSyn.Cre.hGH (#105555). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.Cre.hGH plasmid DNA. hSyn driven Cre expression. These AAV preparations are suitable purity for injection into animals.

   Depositor

   James M. Wilson

   Promoter

   Syn

   Tags

   None

   Available Since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

5. Tubulin-LSSmGFP

   Plasmid

   #186535

   Purpose

   Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to tubulin

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   LSSmGFP
   

   Use

   Tags

   Tubulin

   Expression

   Mammalian

   Mutation

   hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

   Promoter

   CMV

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-EFS-NLS-CasRx-U6-DR-sgRNA_LacZ-DR

   Plasmid

   #154003

   Purpose

   Vector expressed CasRx and sgRNA_LACZ for AAV package

   Depositor

   Hui Yang

   Article

   He et al Protein Cell. 2020 Mar 18. pii: 10.1007/

   Insert

   CasRx
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   EFS

   Available Since

   July 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Str-Golgin84_VSVG-SBP-EGFP

   Plasmid

   #65305

   Purpose

   synchronize trafficking of Golgin-84 from the Golgi apparatus (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Golgin-84 (GOLGA5 Human)
   

   Use

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCXLE-hGLIS1

   Plasmid

   #37625

   Purpose

   Non-integrating (episomal) expression of human GLIS1

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

   Insert

   human GLIS1 (GLIS1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T110A, A187G, I221T

   Promoter

   CAG

   Available Since

   Dec. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLSB-HYG

   Plasmid

   #166699

   Purpose

   Combined sgRNA/Cas9 pLSB vector with hphMX6 (hygromycin) marker. Golden Gate-ready for cloning custom sgRNA sequences.

   Depositor

   Robin Allshire

   Article

   Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do

   Inserts

   tRNA promoter : sgRNA cassette
   adh15 promoter : Cas9 codon-optimised for S. pombe
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   adh15 promoter and tRNA promoter

   Available Since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. GPR77-Tango

    Plasmid

    #66373

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR77 (C5AR2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only