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lenti dCAS-VP64_Blast (Lentiviral Prep)

Viral Prep

#61425-LV

Purpose

Ready-to-use Lentiviral Prep particles produced from lenti dCAS-VP64_Blast (#61425). In addition to the viral particles, you will also receive purified lenti dCAS-VP64_Blast plasmid DNA.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Promoter

EF1a

Tags

None

Available since

July 14, 2016

Availability

Academic Institutions and Nonprofits only
hSERT pcDNA3

Plasmid

#15483

Depositor

Randy Blakely

Article

Ramamoorthy et al Proc Natl Acad Sci U S A. 1993 Mar 15. 9

Insert

hSERT (SLC6A4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only

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SpCas9 TadCBEd

Plasmid

#193835

Purpose

Express TadCBEd (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadA-CDd-SpCas9 D10A-2xUGI

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-Rab7A

Plasmid

#61803

Purpose

Human Rab7A fluorescently tagged with GFP on the N-terminus for mammalian expression

Depositor

Gia Voeltz

Article

Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

Insert

Rab7A (RAB7A Human)

Use

Tags

2x HA and GFP

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pET His6 Mocr TEV LIC cloning vector (1O)

Plasmid

#29658

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

6xHis, Mocr, and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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pmVenus(L68V)-mTurquoise2

Plasmid

#60493

Purpose

Produces a fusion between mTurquoise2 and the FRET acceptor mVenus(L68V). It can be used as a positive control for FRET from mTurquoise2 to mVenus(L68V)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

SYFP2-mTurquoise2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pHIV-Luc-ZsGreen

Plasmid

#39196

Depositor

Bryan Welm

Inserts

Firefly Luciferase Luc2P
ZsGreen

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1-alpha and EF1-alpha

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pET His6 GST TEV LIC cloning vector (2G-T)

Plasmid

#29707

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

6xHis, GST, and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pGWB455

Plasmid

#74837

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with mRFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO V5 HSPA2

Plasmid

#19512

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

heat shock 70kDa protein 2 (HSPA2 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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