165,821 results

1. pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPRE

   Plasmid

   #122151

   Purpose

   Expresses dCas9-SunTag(24X) fusion and a fluorescent indicator BFP.

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

   Insert

   dCas9 fused to SunTag(24x)
   

   Use

   Lentiviral

   Tags

   2xNLS-P2A-BFP and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available Since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF1512

   Plasmid

   #144366

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NKX3-2 (NKX3-2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHBV-Luc

   Plasmid

   #71414

   Purpose

   To see the activity of HBV transcription using Luciferase assay

   Depositor

   Wang-Shick Ryu

   Article

   Cha et al J Gen Virol. 2009 Apr;90(Pt 4):978-86. d

   Insert

   HBV core promoter, enhancer I, enhancer II and luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   HBV

   Available Since

   Dec. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. Str-KDEL_ST-SBP-mCherry

   Plasmid

   #65265

   Purpose

   synchronize trafficking of ST from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)
   

   Use

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 43 of ST

   Promoter

   CMV

   Available Since

   June 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTRE-Fast-FT

   Plasmid

   #31913

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

   Insert

   Fast-FT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   M18V/E34K/K69R/L84W/S149T/A224S compared to mCher…

   Promoter

   PTight Tet-responsive PminCMVd

   Available Since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRF-SrpR

   Plasmid

   #49372

   Depositor

   Christopher Voigt

   Article

   Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

   Insert

   SrpR repressor
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. Cultivarium MACKEREL Library

   Pooled Library

   #208054

   Purpose

   pGL0_151 [RS421] is a library of broad host range prokaryotic promoters-RBS regulatory sequences formatted as a golden gate assembly Level 0 part.

   Depositor

   Cultivarium Tools, Nili Ostrov, Charlie Gilbert

   Article

   Gilbert et al bioRxiv 2024.02.23.581749

   Use

   Synthetic Biology

   Available Since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

8. C1ORF43-3xFLAG

   Plasmid

   #130880

   Purpose

   Expresses human C1ORF43 with C-terminal 3xFLAG tag

   Depositor

   Michael Bassik

   Article

   Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

   Insert

   C1ORF43 (C1orf43 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+)-RIα-EGFP

   Plasmid

   #181840

   Purpose

   GFP-tagged PKA RIα subunit for mammalian expression.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   RIα-EGFP (PRKAR1A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF0677

    Plasmid

    #142192

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PRDM16 (PRDM16 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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