We narrowed to 1,931 results for: cas9 expression vector

1. pZac2.1 U6-SaDMDR7-U6-SaDMDL2

   Plasmid

   #78603

   Purpose

   AAV vector containing gRNAs (for SaCas9) targeting Dmd introns 22 and 23

   Depositor

   Amy Wagers

   Article

   Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

   Insert

   U6-SaDMDR7-U6-SaDMDL2
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

2. pRN3P_T3_ABE8e_IVT

   Plasmid

   #201676

   Purpose

   Plasmid to be used as DNA template for in vitro RNA transcription of the ABE8e base editor (A to G) by T3 RNA polymerase. The plasmid contains optimised 5'UTR and 3'UTR to improve protein expression.

   Depositor

   Timo Otonkoski

   Article

   Jalil et al Am J Hum Genet. 2024 Apr 4;111(4):714-72

   Insert

   ecTadA(8e)-nSpCas9
   

   Use

   CRISPR; Vector for in-vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   T3 promoter

   Available since

   April 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCF142_U6-sgRNA

   Plasmid

   #225960

   Purpose

   U6-sgRNA (recipient). Expresses U6-sgRNA (recipient) for VLP production. This is a recipient vector for cloning of specific SpyCas9 sgRNAs.

   Depositor

   Christof Fellmann

   Article

   Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

   Insert

   U6-sgRNA (recipient)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCDH-V1-rCD20-BSD

   Plasmid

   #209752

   Purpose

   To reconstitute CD20 expression with a specific mRNA isoform (variant 1) after CRISPR-Cas9-mediated knockout of CD20.

   Depositor

   Andrei Thomas-Tikhonenko

   Article

   Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

   Insert

   MS4A1 (MS4A1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   The CCTGGGGGGTCTTCTGATGATCC sequence within the C…

   Promoter

   Available since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCDH-V2-rCD20-BSD

   Plasmid

   #209753

   Purpose

   To reconstitute CD20 expression with a specific mRNA isoform (variant 2) after CRISPR-Cas9-mediated knockout of CD20.

   Depositor

   Andrei Thomas-Tikhonenko

   Article

   Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

   Insert

   MS4A1 (MS4A1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   The CCTGGGGGGTCTTCTGATGATCC sequence within the C…

   Promoter

   Available since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCDH-V3-rCD20-BSD

   Plasmid

   #209754

   Purpose

   To reconstitute CD20 expression with a specific mRNA isoform (variant 3) after CRISPR-Cas9-mediated knockout of CD20.

   Depositor

   Andrei Thomas-Tikhonenko

   Article

   Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

   Insert

   MS4A1 (MS4A1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   The CCTGGGGGGTCTTCTGATGATCC sequence within the C…

   Promoter

   Available since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMVP and pMAGIC Cloning Systems

   Plasmid Kit

   #1000000155

   Purpose

   pMVP and pMAGIC are cloning systems for adenoviral, lentiviral, expression, PiggyBac transposon, and Sleeping Beauty transposon vectors for transgene or RNAi delivery and dCas9-based engineering.

   Depositor

   Christopher Newgard

   Available since

   Sept. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

8. AAVS1-iSAM

   Plasmid

   #211495

   Purpose

   AAVS1 targeting vector containing an all-in-one DOX-inducible system for CRISPRa

   Depositor

   Antonella Fidanza, Lesley Forrester

   Article

   Petazzi et al Elife. 2024 Dec 23;13:RP94884. doi: 10.7

   Inserts

   UniSAM insert
   TET-ON
   Neo Resistance
   Insulator
   

   Use

   CRISPR and Synthetic Biology

   Tags

   T2A-mCherry Tag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a, NA, Splicing acceptor, and TREGV promoter

   Available since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. lentiCRISPRv2_RB1#2

   Plasmid

   #174151

   Purpose

   Lentiviral vector expressing Cas9 and a sgRNA against the human RB1 gene

   Depositor

   Julien Sage

   Article

   Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

   Insert

   RB1 (RB1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. lenti.DTR.GFP

    Plasmid

    #201962

    Purpose

    Lentiviral vector that expresses diptheria toxin receptor (DTR) C-terminally fused to EGFP

    Depositor

    Bernhard Schmierer

    Article

    Fernandez Moro et al Nat Commun. 2023 Aug 18;14(1):5024. doi:

    Insert

    Diptheria Toxin Receptor
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRP_8xgRNA-FLuc

    Plasmid

    #135937

    Purpose

    Firefly luciferase reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activation.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minCMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge