We narrowed to 13,833 results for: Cas9

1. pcDNA3.1-dCas9-CibN

   Plasmid

   #60552

   Purpose

   Expresses dCas9-CibN in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

   Insert

   dCas9-CibN
   

   Use

   CRISPR

   Tags

   FLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   inactivating Cas9 mutations D10A and H840A

   Promoter

   CMV

   Available since

   Jan. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZeo-Cas9-gate

   Plasmid

   #113744

   Purpose

   Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving Zeocin for selection in plants.

   Depositor

   Magdalena Bezanilla

   Article

   Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

   Insert

   Cas9
   

   Use

   CRISPR; Gateway destination vector

   Tags

   Expression

   Mutation

   Promoter

   Z. Mays ubiquitin promoter, 35S promoter

   Available since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSAG1::CAS9-U6::sg290860-6

   Plasmid

   #59855

   Purpose

   Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for CRISPR disruption of TGME49_290860.

   Depositor

   David Sibley

   Article

   Behnke et al Eukaryot Cell. 2014 Dec 5. pii: EC.00229

   Insert

   CRISPR sg290860-6
   

   Use

   CRISPR; ; toxoplasma gondii

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPB-R1R2_EF1adCas9p300_T2A_MS2p65HSF1-IRESbsdpA

   Plasmid

   #113342

   Purpose

   Expresses dCas9-p300 and MS2-p65-HSF1 fusion proteins

   Depositor

   Gavin Wright

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   dCas9-p300core-T2A-MS2-p65-HSF1
   

   Use

   Piggybac

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pST1374-GCN4-dCas9

   Plasmid

   #113023

   Purpose

   Expresses GCN4-dCas9 in mammalian cells

   Depositor

   Xingxu Huang

   Article

   Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

   Insert

   GCN4-dCas9 (GCN4 S. pyogenes, Budding Yeast)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   CMV

   Available since

   July 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. Construct 33 - NFKBRp_Cas9_3xNLS_p2a-puroR

   Plasmid

   #81255

   Purpose

   Lentiviral construct for building stable cell lines expressing Cas9 in the presence of TNFa via puromycin selection

   Depositor

   Timothy Lu

   Article

   Perli et al Science. 2016 Aug 18. pii: aag0511.

   Insert

   Cas9-3xNLS
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. BII-gR-BtW-eSpCas9

   Plasmid

   #133358

   Purpose

   Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   eSpCas9
   

   Use

   Tags

   Flag-tagged eSpCas9

   Expression

   Mammalian

   Mutation

   eSpCas9 mutations

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cas9 gateway compatible

   Plasmid

   #128321

   Purpose

   Cas9 C-terminal gateway fusion

   Depositor

   Alejandro Chavez

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESneopA

   Plasmid

   #112921

   Purpose

   Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Neomycin resistant marker

   Depositor

   Kosuke Yusa

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   EF1a promoter, dCas9VP64, MS2p65HSF1, IRESneo
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAct-Fok1:dCas9

    Plasmid

    #62211

    Purpose

    Expresses Fok1:dCas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous high specificity genome engineering in Drosophila melanogaster.

    Depositor

    Simon Bullock

    Insert

    Fok1-dCas9
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    act5C

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28b-NmCas9-Flag

    Plasmid

    #109030

    Purpose

    Wild type Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

    Depositor

    Yan Zhang

    Article

    Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

    Insert

    CRISPR-associated endonuclease Cas9
    

    Use

    Tags

    Flag Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMCSG7-D16A-NmeCas9

    Plasmid

    #71476

    Purpose

    bacterial pMCSG7 expression vector expressing Nme cas9 nickase with a D16A mutation (RuvC domain) with T7 promoter, N-terminal His tag and TEV site

    Depositor

    Erik Sontheimer

    Article

    Zhang et al Mol Cell. 2015 Oct 15;60(2):242-55. doi:

    Insert

    pMCSG7-D16A-NmeCas9
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    D16A

    Promoter

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST-Cas9-tdTomato

    Plasmid

    #141013

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMCSG7-H588A-NmeCas9

    Plasmid

    #71477

    Purpose

    bacterial pMCSG7 expression vector expressing Nme cas9 nickase with H588A mutation (HNH domain) with T7 promoter, N-terminal His tag and TEV site

    Depositor

    Erik Sontheimer

    Article

    Zhang et al Mol Cell. 2015 Oct 15;60(2):242-55. doi:

    Insert

    pMCSG7-H588A-NmeCas9
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    H588A

    Promoter

    T7

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV_APOBEC1-UdgX-nCas9

    Plasmid

    #163531

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    APOBEC1-UdgX-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRGEB-VirD2-Cas9

    Plasmid

    #139803

    Purpose

    Expressing VirD-Cas9 fusion under ubiquitin promoter

    Depositor

    Magdy Mahfouz

    Article

    Ali et al Commun Biol. 2020 Jan 23;3(1):44. doi: 1

    Insert

    VirD2-Cas9
    

    Use

    Tags

    VirD2-Cas9

    Expression

    Plant

    Mutation

    Promoter

    Ubiquitin

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRS416gT-GalL-Cas9

    Plasmid

    #79903

    Purpose

    pRS416 (URA) + RPR1(TetO) promoter with gRNA locus + Tetracycline Repressor + GalL promoter driven Cas9 for yeast expression

    Depositor

    Ronald Davis

    Article

    Fu et al Nucleic Acids Res. 2016 May 19. pii: gkw

    Inserts

    Cas9
    guide RNA (gRNA)
    Tetracycline Repressor
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GPM1 promoter, GalL, and RPR1 promoter with TetO …

    Available since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQ-dzCas9-NG-Act3.0

    Plasmid

    #158415

    Purpose

    CRISPR-Act3.0 system containing dzCas9-NG-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Insert

    dzCas9-NG-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. px330-CIB1-dCas9

    Plasmid

    #63665

    Purpose

    Mammalian gRNA expression vector also expressing CIB1-dCas9

    Depositor

    Dong Kong

    Article

    Chen et al Theranostics. 2018 Aug 7;8(16):4447-4461

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and CMV

    Available since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX459_gRNA pAS single_hspCas9

    Plasmid

    #193311

    Purpose

    Cas9 from S. pyogenes and U6-pAS single sgRNA (V2.0)

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

    Insert

    Cas9 from S. pyogenes and U6-pAS single sgRNA (V2.0)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    not applicable

    Promoter

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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