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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,493 results for: D10

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Showing: 1281 - 1300 of 2493 results

  6. AAV-AIO-M11-gRNA-EFS-NMS-SadCas9

    Plasmid
    #210710
    Purpose
    This Plasmid express M11 promoter driven SadCas9 specific gRNA and EFS promoter driven NMS transactivation module fused to SadCas9
    Depositor
    Isaac Hilton
    Publication
    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4
    Insert
    SadCas9 specific gRNA and NMS fused to N-terminus of SadCas9 (STAT1, MRTFA Synthetic, Staphylococcus aureus, Human)
    Use
    AAV and CRISPR
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D10A/N580A
    Promoter
    M11
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    210710-plasmid-map-sequence-id-416264

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  7. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)

    Plasmid
    #129028
    Purpose
    inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-mCherry and cloning backbone for sgRNA
    Depositor
    Julia K Polansky
    Publication
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-hudTET1CD, SgRNA cloning site
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    129028-plasmid-map-sequence-id-256131

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  13. pWT063a

    Plasmid
    #107908
    Purpose
    W2m.1-1 plasmid. Contains TRESG-BE3 and is ppart of the CAMERA2m system for cellular recording in mammalian cells
    Depositor
    David Liu
    Publication
    Tang et al Science. 2018 Feb 15. pii: science.aap89
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TRESG-BE3
    Expression
    Mammalian
    Available since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    107908-plasmid-map-sequence-id-207320

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  14. pSABEn

    Plasmid
    #133969
    Purpose
    Expresses ABEn CRISPR base editor and gRNA scaffold in Streptomyces
    Depositor
    Yuhui Sun
    Publication
    Zhong et al bioRxiv 630137
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ABEn
    Use
    CRISPR and Synthetic Biology
    Available since
    Dec. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    133969-plasmid-map-sequence-id-266622

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  15. pRN3P_T3_ABE8e_IVT

    Plasmid
    #201676
    Purpose
    Plasmid to be used as DNA template for in vitro RNA transcription of the ABE8e base editor (A to G) by T3 RNA polymerase. The plasmid contains optimised 5'UTR and 3'UTR to improve protein expression.
    Depositor
    Timo Otonkoski
    Publication
    Jalil et al Am J Hum Genet. 2024 Apr 4;111(4):714-72
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ecTadA(8e)-nSpCas9
    Use
    CRISPR; Vector for in-vitro transcription
    Promoter
    T3 promoter
    Available since
    April 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    201676-plasmid-map-sequence-id-406944

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  17. pSems-leader-SNAPf-mEGFPe-IFNAR1(28-557)

    Plasmid
    #187844
    Purpose
    Expression of SNAPf and mEGFPe-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy
    Depositor
    Jacob Piehler
    Publication
    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    IFNAR1 (IFNAR1 Human)
    Tags
    Ig k-chain leader sequence, SNAPf-tag, and meGFPe…
    Expression
    Mammalian
    Mutation
    meGFPe: asparagine 198 to aspartic acid and tyros…
    Promoter
    CMV
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    187844-plasmid-map-sequence-id-379371

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  18. eTd-CBEa

    Plasmid
    #196603
    Purpose
    Mammalian expression of eTd-CBEa
    Depositor
    Dali Li
    Publication
    Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    bpNLS-TadA-8e (N46L/P29A)-nSpCas9-bpNLS-P2A-UGI-bpNLS
    Tags
    6*His
    Expression
    Mammalian
    Available since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196603-plasmid-map-sequence-id-386796

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Showing: 1281 - 1300 of 2493 results