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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 1281 - 1300 of 34825 results

  7. pAL829

    Plasmid
    #119697
    Purpose
    precursor slow-folding maltodextrin binding protein, MBP or malE, with mutations Y283D, G289C and V347C
    Depositor
    Linda Randall
    Article
    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1
    Insert
    slow-folding maltose ABC transporter periplasmic binding protein, malE, with mutations Y283D, G289C and V347C
    Tags
    none
    Expression
    Bacterial
    Mutation
    mutations Y283D, G289C and V347C
    Promoter
    T5
    Available Since
    Jan. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    119697-plasmid-map-sequence-id-232493

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  8. pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-TALETS3

    Plasmid
    #107301
    Purpose
    Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and TALE-VEGFA-TS3 in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    Truncated SpCas9 (aa 574-1368) fused to FKBP and TALE
    Use
    CRISPR
    Tags
    FKBP, SV40NLS, 3xHA, c-Myc NLS, and TALE_VEGFA-TS…
    Expression
    Mammalian
    Mutation
    Truncated SpCas9 (aa 574-1368) with R1335K mutati…
    Promoter
    CMV IE94
    Available Since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107301-plasmid-map-sequence-id-204785

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  11. pHL1017

    Plasmid
    #62828
    Purpose
    Lim Lab plasmid: AmpR + PConNoHindM8::glgC::gfp::T1T2 terminator + ColE1
    Depositor
    Han Lim
    Article
    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv
    Insert
    gfp
    Use
    Synthetic Biology
    Tags
    m8::glgC leader
    Expression
    Bacterial
    Promoter
    pConNoHind
    Available Since
    Aug. 14, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. pHL1757

    Plasmid
    #53037
    Purpose
    PconNoHindM2:glgC::gfp, PconNoHind:RBS(st3):tetR
    Depositor
    Han Lim
    Article
    Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110
    Inserts
    GFP
    tetR
    Use
    Synthetic Biology
    Tags
    RBS(st3) and glgC leader
    Expression
    Bacterial
    Promoter
    PconNoHindM2 and pConNoHind
    Available Since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. pAAV.Syn.NES-jRGECO1a.WPRE.SV40 (AAV1)

    Viral Prep
    #100854-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.Syn.NES-jRGECO1a.WPRE.SV40 (#100854). In addition to the viral particles, you will also receive purified pAAV.Syn.NES-jRGECO1a.WPRE.SV40 plasmid DNA. Synapsin-driven GECO1a calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:
    Promoter
    Syn
    Available Since
    April 6, 2018
    Availability
    Academic Institutions and Nonprofits only

  14. pAAV.Syn.NES-jRGECO1a.WPRE.SV40 (AAV9)

    Viral Prep
    #100854-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV.Syn.NES-jRGECO1a.WPRE.SV40 (#100854). In addition to the viral particles, you will also receive purified pAAV.Syn.NES-jRGECO1a.WPRE.SV40 plasmid DNA. Synapsin-driven GECO1a calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:
    Promoter
    Syn
    Available Since
    April 17, 2018
    Availability
    Academic Institutions and Nonprofits only

  15. pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40 (AAV1)

    Viral Prep
    #100853-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40 (#100853). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40 plasmid DNA. Synapsin-driven, Cre-dependent GECO1a calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:
    Promoter
    Syn
    Available Since
    Feb. 28, 2018
    Availability
    Academic Institutions and Nonprofits only

  16. pAAV-hSyn1-eYFP (AAV PHP.eB)

    Viral Prep
    #117382-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV-hSyn1-eYFP (#117382). In addition to the viral particles, you will also receive purified pAAV-hSyn1-eYFP plasmid DNA. hSyn-driven eYFP expression. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Viviana Gradinaru
    Article
    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:
    Promoter
    Syn
    Tags
    eYFP
    Available Since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only

  17. pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40 (AAV1)

    Viral Prep
    #100852-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40 (#100852). In addition to the viral particles, you will also receive purified pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent jRGECO1a calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:
    Promoter
    CAG
    Available Since
    July 23, 2018
    Availability
    Academic Institutions and Nonprofits only

  18. pAAV.Syn.NES-jRCaMP1b.WPRE.SV40 (AAV9)

    Viral Prep
    #100851-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV.Syn.NES-jRCaMP1b.WPRE.SV40 (#100851). In addition to the viral particles, you will also receive purified pAAV.Syn.NES-jRCaMP1b.WPRE.SV40 plasmid DNA. Syn-driven jRCaMP1b calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:
    Promoter
    Syn
    Available Since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only

  19. pAAV.CAG.Flex.NES-jRCaMP1b.WPRE.SV40 (AAV1)

    Viral Prep
    #100849-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.CAG.Flex.NES-jRCaMP1b.WPRE.SV40 (#100849). In addition to the viral particles, you will also receive purified pAAV.CAG.Flex.NES-jRCaMP1b.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent NES-jRCaMP1b calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:
    Promoter
    CAG
    Available Since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only

  20. pAAV.CAG.Flex.NES-jRCaMP1a.WPRE.SV40 (AAV1)

    Viral Prep
    #100846-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.CAG.Flex.NES-jRCaMP1a.WPRE.SV40 (#100846). In addition to the viral particles, you will also receive purified pAAV.CAG.Flex.NES-jRCaMP1a.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent jRCaMP1a calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:
    Promoter
    CAG
    Available Since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 1281 - 1300 of 34825 results