Addgene

We narrowed to 3,948 results for: LYC;

Showing: 1281 - 1300 of 3948 results

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AAV hSyn mRuby

Plasmid

#220912

Purpose

Neuronal specific expression of mRuby

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Insert

mRuby

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSF-NgPglO

Plasmid

#198204

Purpose

pSN18 derivative encoding N. gonorrhoeae PglO with C-terminal FLAG epitope tag

Depositor

Michael Jewett

Article

Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

Insert

NgPglO

Use

Tags

Flag tag

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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OM14-2EGFP-UG74-ter

Plasmid

#184758

Purpose

Marker for yeast mitochondria. Integration of 2xGFP tag at OM14 C terminus. Uses antibiotic resistance marker natMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

OM14 (OM14 Budding Yeast)

Use

Tags

2EGFP

Expression

Yeast

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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SEC7-2EGFP-ter-NAT

Plasmid

#184764

Purpose

Marker for yeast late Golgi/early endosome . Integration of 2xGFP tag at SEC7 C terminus. Uses antibiotic resistance marker natMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

SEC7 (SEC7 Budding Yeast)

Use

Tags

2EGFP

Expression

Yeast

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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IRE1-2EGFP-ter-kanMX4

Plasmid

#184766

Purpose

Integration of 2xGFP tag at IRE1 C terminus. Potential indicator of UPR activation. Uses antibiotic resistance marker kanMX4.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

IRE1 (IRE1 Budding Yeast)

Use

Tags

2EGFP

Expression

Yeast

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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CIT1-2EGFP-ter-URA3

Plasmid

#184767

Purpose

Marker for yeast mitochondria. Integration of 2xGFP tag at CIT1 C terminus. Uses auxotrophic marker URA3(Candida albicans).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

CIT1 (CIT1 Budding Yeast)

Use

Tags

2EGFP

Expression

Yeast

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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EMC1-2EGFP-ter-NAT

Plasmid

#184763

Purpose

Marker for yeast endoplasmic reticulum (ER). Integration of 2xGFP tag at EMC1 C terminus. Uses antibiotic resistance marker natMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

EMC1 (EMC1 Budding Yeast)

Use

Tags

2EGFP

Expression

Yeast

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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ACH1-2EGFP-ter-URA3

Plasmid

#184769

Purpose

Marker for yeast mitochondria. Integration of 2xGFP tag at ACH1 C terminus. Uses auxotrophic marker URA3(Candida albicans).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

ACH1 (ACH1 Budding Yeast)

Use

Tags

2EGFP

Expression

Yeast

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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AFG3-2EGFP-ter-URA3

Plasmid

#184770

Purpose

Marker for yeast mitochondria. Integration of 2xGFP tag at AFG3 C terminus. Uses auxotrophic marker URA3(Candida albicans).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

AFG3 (AFG3 Budding Yeast)

Use

Tags

2EGFP

Expression

Yeast

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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SYS1-2EGFP-ter-URA3

Plasmid

#184771

Purpose

Marker for yeast early Golgi. Integration of 2xGFP tag at SYS1 C terminus. Uses auxotrophic marker URA3(Candida albicans).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

SYS1 (SYS1 Budding Yeast)

Use

Tags

2EGFP

Expression

Yeast

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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SEC7-2Katushka-ter-Hygromycin

Plasmid

#184772

Purpose

Red marker for yeast late Golgi/early endosome. Integration of 2xKatushka tag at SEC7 C terminus. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

SEC7 (SEC7 Budding Yeast)

Use

Tags

2Katushka

Expression

Yeast

Mutation

Promoter

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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PKC1-2GFP-ter-TRP1

Plasmid

#184773

Purpose

Integration of 2xGFP tag at PKC1 C terminus. Uses auxotrophic marker TRP1(Kluyveromyces lactis).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

PKC1 (PKC1 Budding Yeast)

Use

Tags

2EGFP

Expression

Yeast

Mutation

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-ammB3

Plasmid

#167815

Purpose

Expresses His6-tagged AmmB3 in E. coli

Depositor

Wilfred van der Donk

Article

Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

Insert

AmmB3

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-ammC1

Plasmid

#167816

Purpose

Expresses His6-tagged AmmC1 in E. coli

Depositor

Wilfred van der Donk

Article

Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

Insert

AmmC1

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAct-GST-H3(K27Q)

Plasmid

#182843

Purpose

a K27Q point mutation (AAG/CAG) of Drosophila histone H3 was generated from pAct-GST-H3. Expression in S2 cells.

Depositor

Peter Harte

Article

Tie et al Development. 2009 Sep;136(18):3131-41. d

Insert

GST-histone H3 fusion

Use

Tags

GST

Expression

Insect

Mutation

Promoter

Actin5C

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pAct-GST-H3

Plasmid

#182842

Purpose

A PCR product encoding GST-H3 (Drosophila histone H3) was was inserted into a modified pAct-5c vector by Sac I and Sal I sites. Expression in S2 cells.

Depositor

Peter Harte

Article

Tie et al Development. 2009 Sep;136(18):3131-41. d

Insert

GST-histone H3 fusion

Use

Tags

GST

Expression

Insect

Mutation

Promoter

Actin5C

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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Pb mD3S4-dN

Plasmid

#180252

Purpose

P. berghei D3 (502-617) construct with N516T, S533N and N539Q mutations to abolish N-glycosylation was used for crystallization.

Depositor

Timothy Springer

Article

Feng et al Elife. 2021 Dec 23;10. pii: 74707. doi:

Insert

D3 of P. berghei HAP2 with N-glycosylation sites mutated

Use

Tags

6His

Expression

Mammalian

Mutation

N516T, S533N and N539Q

Promoter

Available since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pThuAsol

Plasmid

#171675

Purpose

pThuAsol expression in E. coli

Depositor

Wilfred van der Donk

Article

Fujinami et al Cell Chem Biol. 2021 Dec 16;28(12):1740-

Insert

SUMO-TEV-ThuAsol

Use

Tags

His-tag and SUMO-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pHCKan-T7-CBD-hGLY

Plasmid

#134940

Purpose

T7 based expression of human glycine acyltransferase with an N-terminal chitin binding domain

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

human glycine acyltransferase 2 (GLYATL2 )

Use

Tags

chitin binding domain

Expression

Bacterial

Mutation

Promoter

t7

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pst39-NST-clone14

Plasmid

#133421

Purpose

S. cerevisiae Nip1 (with C-terminal TEV cleavage site and strep-II tag), Sui1 and Tif5B6 (with N-terminal 7xHis tag and TEV cleavage set) coding sequences with intervening ribosome binding sites and other cis-elements for expression as a polycistron, T7 theta 10 promoter (and corresponding terminator after polycistron).

Depositor

Gerhard Wagner

Insert

NIP1/SUI1/TIF5 (NIP1, TIF5, SUI1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

ORF for Nip1 encodes aa 4-156 (not complete ORF),…

Promoter

Available since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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