We narrowed to 6,363 results for: wus
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Plasmid#214062Purposeinducible PE-Plus system for controllable prime editing; this plasmid is used to insert PE-Plus prime editor (PEmax-P2A-hP53DD-IRES-MLH1dn) in one allele of AAVS1 locusDepositorInsertPE-Plus
UseTagsExpressionMammalianMutationPromoterTRE-tightAvailable SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-PA-Rac1-C450A
Plasmid#22028PurposeExpression of Rac1 fused with light-insensitive LOV2 (C450A mutation)DepositorInsertPA-Rac1-C450A (RAC1 Avena sativa (oat), Human)
UseTags6xHis and mCherryExpressionBacterial, Insect, and Mamm…MutationLOV(Leu404-Leu546, C450A). Rac1 starts at Ile4 an…PromoterCMV, p10Available SinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-PA-Rac1-T17N
Plasmid#22029PurposeExpression of photoactivatable Rac1 (dominant-negative mutant).DepositorInsertPA-Rac1-T17N (RAC1 Avena sativa (oat), Human)
UseTags6xHis and mCherryExpressionBacterial, Insect, and Mamm…MutationLOV(Leu404-Leu546). Rac1 starts at Ile4 and conta…PromoterCMV, p10Available SinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCas-dCas12m
Plasmid#192275PurposeEncodes MmdCas12m (D485A) under a constitutive promoterDepositorInsertMmdCas12m
UseCRISPRTagsExpressionBacterialMutationD485APromoterPJ23108Available SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 E-cadherin shRNA/E-cadherin GFP-IRES-HA-H-Ras V12
Plasmid#101285PurposeLentiviral expression ,was generatedby recombining bothIRES an HA-tagged H-Ras V12 into SbfI site of pLL5.0 Ecadherin shRNA/mEcad-GFPDepositorInsertE-Cadherin-GFP-H-Ras
UseLentiviralTagsGFPExpressionMammalianMutationH-Ras V12PromoterAvailable SinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
p23-mNeongreen-DDX21 WT
Plasmid#179228Purposeoverexpression in human cellsDepositorAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCas-Cas12m
Plasmid#192274PurposeEncodes MmCas12m under a constitutive promoterDepositorInsertMmCas12
UseCRISPRTagsExpressionBacterialMutationPromoterPJ23108Available SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-RFP entry
Plasmid#192278PurposeEntry vector for cloning MmCas12m spacers. RFP flanked by MmCas12m CRISPR repeats can be digested out with BbsI.DepositorInsertRFP flanked by type V-M CRISPR repeat sequences
UseCRISPRTagsExpressionBacterialMutationPromoterPJ23119Available SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas-MmdCas12m-CBE1
Plasmid#192284PurposeMmdCas12m-CBE1 expressed under a constitutive promoterDepositorInsertMmdCas12m-CDA-UGI
UseCRISPRTagsExpressionBacterialMutationD485APromoterPJ23108Available SinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
p23-mNeongreen-DDX21 WT-mRuby3
Plasmid#179225Purposeoverexpression in human cellsDepositorInsertDDX21 (DDX21 Human)
UseTagsmNeongreen and mRuby3ExpressionMammalianMutationPromoterEF-1alphaAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
tetO-Bcl2-IRES-tdtomato(TET005)
Plasmid#117857PurposeThis plasmid expresses Bcl2 under the activation of tTA trans-activator along with fluorescent marker tdTomato.DepositorAvailable SinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mPA-GFP-PA-Rac1
Plasmid#22022PurposeExpression of photoactivatable Rac1DepositorInsertPA-Rac1 (RAC1 Avena sativa (oat), Human)
UseTags6X His and mPA-GFPExpressionBacterial, Insect, and Mamm…MutationRac1 starts at I4 and contains mutations Q61L, E9…PromoterCMV, p10Available SinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTarget: Ptaq-RFP_PlacIq-GFP
Plasmid#192280PurposeRFP expressed under constitutive promoter Ptaq and GFP expressed under constitutive promoter PlaciqDepositorInsertsRFP
GFP
UseTagsExpressionBacterialMutationPromoterPlacIq and PtaqAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
p23-mRuby3-DDX21 WT
Plasmid#179231Purposeoverexpression in human cellsDepositorAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-PA-Rac1-C450A
Plasmid#22025PurposeExpression of Rac1 fused with light-insensitive LOV2 (C450A mutation)DepositorInsertPA-Rac1-C450A (RAC1 Avena sativa (oat), Human)
UseTags6X HisExpressionBacterial, Insect, and Mamm…MutationLOV domain contains light-insensitive mutation C4…PromoterCMV, p10Available SinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-P2A-hP53DD
Plasmid#214084PurposeMammalian expression of SpCas9 PEmax prime editor with P2A-hP53DDDepositorInsertPEmax-P2A-hP53DD (TP53 Synthetic, Human, SpCas9 is from Streptococcus pyogenes; MMLV_RT is from the Moloney murine leukemia virus)
UseTagsExpressionMammalianMutationPromoterCMVAvailable SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-PA-Rac1-I539E
Plasmid#22026PurposeExpression of photoactivatable Rac1 ('lit' state mutant)DepositorInsertPA-Rac1-I539E (RAC1 Avena sativa (oat), Human)
UseTags6X HisExpressionBacterial, Insect, and Mamm…MutationLOV domain contains 'lit state' mutatio…PromoterCMV, p10Available SinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
p23-mNeongreen-DDX21 DAT-mRuby3
Plasmid#179226Purposeoverexpression in human cellsDepositorInsertDDX21 (DDX21 Human)
UseTagsmNeongreen and mRuby3ExpressionMammalianMutationG234D, K235APromoterEF-1alphaAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTarget-RFP-GFP operon
Plasmid#192279PurposeRFP GFP fusion protein expressed under a constitutive promoterDepositorInsertRFP GFP
UseTagsExpressionBacterialMutationPromoterPlacIqAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-Kir2.1-pA-CMV-mRFP-pA(AV39)
Plasmid#117856PurposeThis plasmid expresses Kir2.1 along with fluorescent marker RFP under the control of CMV promoter. The vector also supports the construction of adenovirus using ADeasy system.DepositorAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only