Addgene

We narrowed to 7,585 results for: Lif;

Showing: 1301 - 1320 of 7585 results

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pPBpuro-COX2-EBFP2

Plasmid

#184720

Purpose

Exogenous COX2

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

COX2-EBFP2 (PTGS2 Human, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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MMLV-CAG-G_oT7pol-IRES-BSD

Plasmid

#172369

Purpose

Generation of oT7pol-expressing stable cell lines, resistant to the antibiotic Blasticidin

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

oT7pol + BSD

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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MMLV-CAG-SADB19_oG-IRES-Puro

Plasmid

#172367

Purpose

Generation of stable cell lines expressing the rabies optimized glycoprotein (oG), resistant to the antibiotic Puromycin

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

oG + Puro

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLIC-lyn11-myc-LOV2GIVe

Plasmid

#170623

Purpose

For the mammalian expression of mLOV2GIVe

Depositor

Mikel Garcia-Marcos

Article

Garcia-Marcos et al Elife. 2020 Sep 16;9. pii: 60155. doi: 1

Insert

Lyn11-myc-LOV2GIVe

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)_NAD-Snifit

Plasmid

#113658

Purpose

Plasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring NAD+ concentration

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NAD-Snifit

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-TK-Firefly-Control

Plasmid

#122277

Purpose

Lentiviral vector for stable expression of Firefly luciferase.

Depositor

Demián Cazalla

Article

Gorbea et al Elife. 2019 Sep 20;8. pii: 50530. doi: 1

Insert

Firefly luciferase

Use

Lentiviral and Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

Thymidine kinase

Available since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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SAC1deltaTMD-FKBP-mCherry

Plasmid

#108123

Purpose

Recruitable cytosolic SAC1

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

SACM1L(1-520):FKBP1A(3-108):mCherry (SACM1L Human)

Use

Tags

Expression

Mammalian

Mutation

SACM1L(1-520)

Promoter

CMV

Available since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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OSCA4.1

Plasmid

#136597

Purpose

Expresses the mechanosensitive channel OSCA4.1 in mammalian cells

Depositor

Ardem Patapoutian

Article

Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

Insert

OSCA4.1 (AT4G35870 Mustard Weed)

Use

Tags

pIRES2-mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-wtSpCas9-H840A

Plasmid

#80453

Purpose

SpCas9 mammalian expression vector

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Insert

SpCas9_H840A

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Aug. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TetO-U2B''-fSNAPc

Plasmid

#106988

Purpose

open reading frame of U2B'' inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites

Depositor

Melissa Moore

Article

Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

Insert

UB2" (SNRPB2 Human)

Use

Tags

fSNAP

Expression

Mutation

Promoter

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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FKBP-AP180-GFP

Plasmid

#186577

Purpose

Expression of the fusion protein FKBP-AP180-GFP. Uses rat AP180 residues 328-896.

Depositor

Stephen Royle

Article

Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

Insert

AP180 (Snap91 Rat)

Use

Tags

FKBP N-terminal GFP C-terminal

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-SAC1-FKBP

Plasmid

#108129

Purpose

Recruitable SAC1

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:SACM1L:FKBP1A(3-108) (SACM1L Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g7

Plasmid

#80790

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 7/17/27 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-mTagBFP-T2A-TetOn3G

Plasmid

#179888

Purpose

Expresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

TetOn3G

Use

Lentiviral

Tags

mTagBFP

Expression

Bacterial and Mammalian

Mutation

Promoter

EF1a

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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acta1-mRuby2-dGBP

Plasmid

#163855

Purpose

Zebrafish muscle specific expression of mRuby2-dVHH[GFP]

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

dVHH[GFP]

Use

In vitro transcription of rna

Tags

Ruby2

Expression

Mutation

Promoter

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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tCRMod-GGSGGS9

Plasmid

#111762

Purpose

Expresses soluble Clover-mRuby2 tension sensor module with 9-repeat GGSGGS extensible domain

Depositor

Brenton Hoffman

Article

LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

Insert

Clover-GGSGGS9-mRuby2 fusion

Use

Tags

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pET151-Vps4

Plasmid

#87733

Purpose

Bacterial expression of full-length S. cerevisiae Vps4

Depositor

Chris Hill

Article

Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

Insert

Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)

Use

Tags

6xHis-V5-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCEP-Puro-TetOn-L1RP-ORF1_R261A_Halo-GFPai

Plasmid

#202569

Purpose

Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the R261A mutation and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR

Depositor

Liam Holt

Article

Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

Insert

LINE-1

Use

Tags

GFP-AI retrotransposition reporter and HaloTag7 o…

Expression

Mammalian

Mutation

Changed ORF1p Arginine 261 to Alanine

Promoter

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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Plas-gRNA-Z3EV

Plasmid

#157659

Purpose

GFP gene and gRNA under Z3EV expression control (estradiol sensor), with Z3EV transcription factor to insert in the genome.

Depositor

Frank Albert

Article

Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1

Insert

GFP-gRNA NatMX Z3EV

Use

Insert storage (replicative in e. coli)

Tags

Expression

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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raACR-pmCerulean3-N1

Plasmid

#204959

Purpose

Expression of the MsACR1 mutant raACR as a fusion protein with mCerulean3 in mammalian cells

Depositor

Peter Hegemann

Article

Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

Insert

raACR

Use

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

CMV (+enhancer)

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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