We narrowed to 7,766 results for: loxP

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pT-GEM(2)

Plasmid

#62894

Purpose

Trojan-Gal4 Expression Module in Phase 2. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

Depositor

Benjamin White

Publication

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Type

Empty backbone

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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EF1a_RUNX1_P2A_Hygro_Barcode

Plasmid

#120475

Purpose

Barcoded lentiviral vector to express RUNX1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Publication

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

RUNX1 (RUNX1 Human)

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-Ef1a-DIO-ChETA-TdTomato-WPRE-pA

Plasmid

#37755

Purpose

Cre-dependent expression of ChETA-tdTomato, for optogenetic excitation

Depositor

Bernardo Sabatini

Publication

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

ChETA-TdTomato

Use

AAV and Cre/Lox; Cre-on

Tags

TdTomato

Promoter

EF1a

Available since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJJR81

Plasmid

#75029

Purpose

tagBFP2^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Mike Boxem

Insert

tagBFP2^SEC^3xFlag

Use

CRISPR

Tags

3xFLAG and C. elegans codon optimized tagBFP2

Expression

Worm

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pTriEx-mCherry-LOV2 I427T

Plasmid

#81035

Purpose

Mammalian expression of LOV I427T, a mutant with super short relaxation time

Depositor

Klaus Hahn

Publication

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LOV I427T

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

I427T

Available since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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JME4580

Plasmid

#129659

Purpose

HPHex_CrisprCas9-yl_RFP: CAS9 vector with Hygromycinex marker for gRNA cloning using GoldenGate

Depositor

Tristan Rossignol

Publication

Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLPCXU6PT7SK

Plasmid

#27549

Depositor

Kathleen Collins

Publication

Hogg et al RNA. 2007 Jun . 13(6):868-80.

Insert

U6PT7SK (RN7SK Human)

Use

Retroviral

Expression

Mammalian

Available since

April 20, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pJZC78

Plasmid

#62339

Purpose

sgRNA + 1x COM with COM-KRAB effector for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Publication

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 1x COM binding module
COM-KRAB

Use

Lentiviral

Tags

KRAB

Expression

Mammalian

Mutation

Targets sv40 promoter, sequence: CATACTTCTGCCTGCT…

Promoter

CMV and U6

Available since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pUG6SP-tTA'

Plasmid

#41030

Depositor

Michael Nick Boddy

Publication

Zilio et al Yeast. 2012 Aug 29. doi: 10.1002/yea.292

Insert

tTA' (tetR'-VP16)

Available since

Dec. 14, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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[#GS16-LV] Syn1-MitoTRACER - Donor

Plasmid

#233565

Purpose

Lentiviral construct of the MitoTRACER genetic reporter to be expressed in the donor cells under the Synapsin1 promotor

Depositor

Simon Grelet

Publication

Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

Insert

MitoTRACER-Donor

Use

Lentiviral and Synthetic Biology

Tags

GFP11 and HA Tag

Expression

Mammalian

Available since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_EF1a_Flag_TP53_WT

Plasmid

#183112

Purpose

Expresses flag-tagged p53 in mammalian cells

Depositor

William Kaelin

Publication

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

TP53 (TP53 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

See Depositor Comments Below

Promoter

EF1a

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-CAG-FLEX-jGCaMP7b-WPRE

Plasmid

#104497

Purpose

AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switch

Depositor

Douglas Kim, GENIE Project

Publication

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Insert

jGCaMP7b

Use

AAV and Cre/Lox

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Promoter

CAG

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pWalium20-10XUAS-3XFLAG-dCas9-VPR

Plasmid

#78897

Purpose

Expresses 3XFLAG-dCas9-VPR (human codon) under 10XUAS control, for in vivo CRISPRa

Depositor

Norbert Perrimon

Publication

Lin et al Genetics. 2015 Oct;201(2):433-42. doi: 1

Insert

dCas9-VPR

Tags

3xFLAG

Expression

Insect

Promoter

10XUAS

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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PITX3-eGFP-PGK-Puro

Plasmid

#31942

Depositor

Rudolf Jaenisch

Publication

Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PITX3 (PITX3 Human)

Use

Targeting donor

Tags

eGFP

Available since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pF2-DTA

Plasmid

#42521

Depositor

Terry Magnuson

Publication

Jang et al PLoS One. 2013;8(2):e57075. doi: 10.1371

Insert

EF1a-DTA

Use

Mouse Targeting

Available since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMaCTag-P28

Plasmid

#120039

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 3xHA

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

3xHA

Use

Pcr template

Tags

3xHA

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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FUW-SM

Plasmid

#20330

Depositor

Rudolf Jaenisch

Publication

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Sox2-F2A-cMyc

Use

Lentiviral

Expression

Mammalian

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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AAV phSyn1(S)-FLEX-EGFP-WPRE

Plasmid

#51504

Purpose

Can be used to generate AAV virus that will express EGFP in the presence of Cre in neurons from the synapsin promoter

Depositor

Hongkui Zeng

Publication

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Insert

EGFP

Use

AAV

Promoter

phSyn1

Available since

May 1, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pBIG1b

Plasmid

#80612

Purpose

pBIG1b - Step1 vector b (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Publication

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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UAS:Zebrabow-B

Plasmid

#118216

Purpose

For broad gal4 inducible expression of Brainbow in zebrafish

Depositor

Albert Pan, Alex Schier

Publication

Pan et al Development. 2013 Jul;140(13):2835-46. d

Insert

Brainbow 1.0 L

Use

Zebrafish expression

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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