Addgene

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pEGFP-N1-FUS/TLS-FLAGC

Plasmid

#60362

Purpose

Plasmid for expression of FLAG-GFP tagged human FUS/TLS (C-terminal tag). Confers resistance to G418.

Depositor

Patrick Calsou, Sébastien Britton

Article

Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

Insert

FUS (FUS Human)

Use

Tags

FLAG and GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCS2-nCas9n-nanos 3’UTR

Plasmid

#62542

Purpose

expression of a germ-line targeted, codon optimized Cas9 for genome-editing in zebrafish

Depositor

Antonio Giraldez

Article

Moreno-Mateos et al Nat Methods. 2015 Aug 31. doi: 10.1038/n

Insert

nanos 3' UTR (nanos3 Zebrafish)

Use

CRISPR

Tags

Expression

Mutation

3' UTR only

Promoter

SP6

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

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mCherry-PCNA

Plasmid

#215117

Purpose

Expresses mCherry-PCNA in mammalian cells from a lentiviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

PCNA (PCNA Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP hsp70

Plasmid

#15215

Depositor

Lois Greene

Article

Zeng et al J Cell Sci. 2004 Oct 1. 117(Pt 21):4991-

Insert

hsp70 (HSPA1A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 15, 2007

Availability

Academic Institutions and Nonprofits only

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pLV_EF1aUBA3_IRES_Puro

Plasmid

#235660

Purpose

human UBA3 cloned into pLV-EF1a-IRES-Puro vector (Addgene: 85132)

Depositor

Lorenz Studer

Article

Saurat et al Cell Stem Cell. 2024 Aug 1;31(8):1162-11

Insert

UBA3 (UBA3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCI HA NEDD4L

Plasmid

#27000

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

NEDD4L (NEDD4L Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-ChR2-GFP

Plasmid

#26929

Purpose

AAV-mediated expression of ChR2-GFP for neuronal excitation

Depositor

Edward Boyden

Article

Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

Insert

ChR2

Use

AAV; Adeno-associated virus

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-Gm-ecfp

Plasmid

#65030

Purpose

GmR mini-Tn7 vector, mobilizable, for CFP tagging bacteria

Depositor

Herbert Schweizer

Article

Choi et al Nat Protoc. 2006;1(1):153-61.

Insert

ecfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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Dyn2-pmCherryN1

Plasmid

#27689

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Dynamin 2 (DNM2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

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pSF463

Plasmid

#167671

Purpose

Intermediate vector for two gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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