163,819 results

1. R777-E139 Hs.NF1

   Plasmid

   #70423

   Purpose

   Gateway ORF clone of codon optimized human NF1 [NM_000267.3] with stop codon (for native or N-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   NF1 (NF1 Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-(BbsI)_CBh-Cas9-T2A-mCherry

   Plasmid

   #64324

   Purpose

   Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptide

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA cassette
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti6.2_mTagBFP2

   Plasmid

   #113725

   Purpose

   Expresses the fluorescent protein mTagBFP2 in a lentiviral backbone

   Depositor

   Vanessa LaPointe

   Article

   Wieland et al Front Bioeng Biotechnol. 2021 Sep 9;9:72

   Insert

   mTagBFP2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. qTAG-N-Blast-ultraID

   Plasmid

   #207682

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-ultraID cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. SPLICS LY-MT Short P2A

   Plasmid

   #213614

   Purpose

   Detect the short-range Lysosome-Mitochondria contact

   Depositor

   Tito Calì

   Article

   Giamogante et al Nat Commun. 2024 Feb 19;15(1):1516. doi:

   Insert

   SPLICS LY-MT Short P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL0_13 [pIP501 ORI]

   Plasmid

   #198927

   Purpose

   Level 0 part

   Depositor

   Nili Ostrov, Charlie Gilbert, Cultivarium Tools

   Article

   Gilbert et al bioRxiv 2023.05.19.541510

   Insert

   pIP501 ORI
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. TL024_lenti_hSyn:HaloTag-GluA1

   Plasmid

   #192517

   Purpose

   Expression of AMPA GluA1 with N terminal HaloTag fusion

   Depositor

   Adam Cohen

   Article

   Kim et al bioRxiv. 2023 May 29:2023.05.26.541296.

   Insert

   HaloTag-GluA1
   

   Use

   Lentiviral

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. SIRT2 Flag

   Plasmid

   #13813

   Purpose

   Mammalian expression of human SIRT2 with flag tag

   Depositor

   Eric Verdin

   Article

   North et al Mol Cell. 2003 Feb . 11(2):437-44.

   Insert

   SIRT2 (SIRT2 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Transcript variant 2

   Promoter

   Available since

   Feb. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-hNurr1-NE

   Plasmid

   #102363

   Purpose

   Overexpression of NR4A2 nuclear receptor subfamily 4 group A member 2 in mammalian cells

   Depositor

   Shu Leong Ho

   Insert

   NR4A2 nuclear receptor subfamily 4 group A member 2 (NR4A2 Human)
   

   Use

   Tags

   NE

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLQ8001 - pCMV-R8.91 (D64V)

    Plasmid

    #202687

    Purpose

    R8.91 plasmid with mutated integrase (D64V)

    Depositor

    Stanley Qi

    Article

    Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

    Insert

    gag pol
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D64V

    Promoter

    Available since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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