We narrowed to 1,974 results for: Ncl
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Plasmid#130964PurposeEngineered sgRNA-LEB2 (2 BoxB aptamers) generator circuit including promoter Plux2DepositorInsertsgRNA-LEB2
UseSynthetic BiologyExpressionBacterialPromoterPlux2Available SinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY124
Plasmid#130946PurposeEngineered sgRNA-G6-2MS2 (2 MS2 aptamers) generator circuit including promoter PbadDepositorInsertsgRNA-G6-2MS2
UseSynthetic BiologyExpressionBacterialPromoterPbadAvailable SinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY112
Plasmid#130944PurposeEngineered sgRNA-LEB5 (2 BoxB aptamers) generator circuit including promoter Plux2DepositorInsertsgRNA-LEB5
UseSynthetic BiologyExpressionBacterialPromoterPlux2Available SinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY107
Plasmid#130943PurposeEngineered sgRNA-LEA5 (2 BoxB aptamers) generator circuit including promoter Plux2DepositorInsertsgRNA-LEA5
UseSynthetic BiologyExpressionBacterialPromoterPlux2Available SinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY105
Plasmid#130939PurposeEngineered sgRNA-LEA3 (2 BoxB aptamers) generator circuit including promoter Plux2DepositorInsertsgRNA-LEA3
UseSynthetic BiologyExpressionBacterialPromoterPlux2Available SinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY106
Plasmid#130941PurposeEngineered sgRNA-LEA4 (2 BoxB aptamers) generator circuit including promoter Plux2DepositorInsertsgRNA-LEA4
UseSynthetic BiologyExpressionBacterialPromoterPlux2Available SinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS_KS_attB2_SA(2)_T2AGeneSwitch
Plasmid#125216PurposeArtificial phase 2 exon to include a spliced T2A-GeneSwitch effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a geneDepositorInsertGeneSwitch
UseOtherTagsT2AAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS_KS_attB2_SA(2)_T2AGAL80
Plasmid#125219PurposeArtificial phase 2 exon to include a spliced T2A-GAL80 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a geneDepositorInsertGAL80
UseOtherTagsT2AAvailable SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS_KS_attB2_SA(1)_T2AGeneSwitch
Plasmid#125215PurposeArtificial phase 1 exon to include a spliced T2A-GeneSwitch effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a geneDepositorInsertGeneSwitch
UseOtherTagsT2AAvailable SinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
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GLRX3
Plasmid#39036PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertGLRX3 (GLRX3 Human)
TagsHis TevExpressionBacterialMutationA PCR artifact adds a C-terminal sequence includi…Available SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLG-deltaA
Plasmid#33151DepositorInsertRP51A-synthetic minimal intron (RPS17A Budding Yeast)
TagsLacZExpressionYeastMutationThe RP51A sequence was modified by digestion with…Available SinceDec. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFloxin-Ofd1-Myc-S437R
Plasmid#24577DepositorInsertOfd1 (Ofd1 Mouse)
UseCre/Lox and Mouse TargetingTagsMycMutationSerine 437 changed to Arginine (codon AGT to CGT)…Available SinceMay 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
p077 pCS xTRPS1 (M)
Plasmid#11047DepositorInsertTRPS1 mut (trps1 Frog)
UseMammalian, avian, and zebrafishMutationTwo Cys residues in the GATA-type zinc finger wer…Available SinceDec. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pKK-BiFC-Venus
Plasmid#105804PurposeConcomitant expression of two proteins in fusion with fragments of Venus protein. Protein interactions studies by bimolecular fluorescence complementation (BiFC), including BiFC-FRET assays.DepositorTypeEmpty backboneUseFlp-in competentTagsVN173 (I152L); VC155ExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
DNMT3A-dCas9
Plasmid#100090PurposeDNMT3A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorInsertDNMT3A (DNMT3A Human)
UseCRISPRTags3XFLag-NLS-DNMT3A-dCas9-NLSExpressionMammalianMutationincludes aa 602-912PromoterCMVAvailable SinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTubb3-MC
Plasmid#87112Purposeminicircle parental backbone plasmid including GFP knock-in donor targeting Tubb3 gene and one cutting site for HITIDepositorInsertGFP
UseCRISPRAvailable SinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMDC32B-AtTAS1c-D2-B/c-AtMIR173
Plasmid#137885PurposePlant expression vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor downstream 3'D1[+]. Contains AtMIR173 for syntasi expression in any plant species.DepositorInsertsAtTAS1c-D2-B/c
2x35S-AtMIR173-Tnos
ExpressionPlantMutationA. thaliana TAS1c precursor sequence including a …Available SinceMay 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-eGFP-NLS-RNF168 delta RING
Plasmid#133983Purposemammalian expression vector of eGFP tagged RNF168 where the N-terminus of RNF168 has been deleted (including RING domain)DepositorInsertRNF168 (RNF168 Human)
TagseGFPExpressionMammalianMutationdeletion of RING; siRNA resistant sequence: GAGGA…PromoterCMVAvailable SinceNov. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAi14-GFPNLS-MC
Plasmid#87114Purposeminicircle parental backbone plasmid including GFPNLS-pA knock-in donor and one cutting site for HITIDepositorInsertGFPNLSPpA
UseCRISPRPromoternEFAvailable SinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only