We narrowed to 2,869 results for: human cdnas

1. pcDNA3.1 2xFLAG-2xSTREP RAD51 (F129A)

   Plasmid

   #158952

   Purpose

   Express human RAD51(F129A) mutant in mammalian cells

   Depositor

   Michele Pagano

   Article

   Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

   Insert

   RAD51 (RAD51 Human)
   

   Use

   Tags

   2X FLAG 2X STREP

   Expression

   Mammalian

   Mutation

   changed phenylalanine 129 to alanine

   Promoter

   CMV

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNATM3.1/V5-His TOPO-PKCi-K274W

   Plasmid

   #55690

   Purpose

   expresses dominant negative human PKCi tagged with V5

   Depositor

   Pedro Salas

   Article

   Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54.

   Insert

   Homo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   A120E and K274W

   Promoter

   CMV

   Available since

   Aug. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pcDNATM3.1/V5-His TOPO-PKCi-A120E

   Plasmid

   #55687

   Purpose

   expresses constitutively active human PKCi tagged with V5

   Depositor

   Pedro Salas

   Article

   Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54.

   Insert

   Homo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   A120E

   Promoter

   CMV

   Available since

   July 28, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3.1(+) ZKSCAN1 MCS-HIPK3 Exon

   Plasmid

   #69905

   Purpose

   Expresses the human HIPK3 exon 2 circular RNA in mammalian cells

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   HIPK3 (HIPK3 Fly, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Expresses aa1-194 of HIPK3

   Promoter

   CMV

   Available since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA4/TO CD16.7-h-PKD1 FLM R4114A

   Plasmid

   #83461

   Purpose

   Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and a mutation in the first Calmodulin binding domain (503)

   Depositor

   Thomas Weimbs

   Article

   Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

   Insert

   Polycystin-1 (PKD1 Human)
   

   Use

   Tags

   CD16.7

   Expression

   Mammalian

   Mutation

   Amino-acids 4078-4303, R4113A

   Promoter

   Available since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3-IgL-C99-T4L-rTA

   Plasmid

   #177840

   Purpose

   Tango assay

   Depositor

   Karsten Melcher

   Article

   Yan et al Acta Pharmacol Sin. 2017 Oct;38(10):1412

   Insert

   C terminal 99 amino acid fragment of human Alzheimer Amyloid beta precursor Protein (APP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1-myc:Skp1(dC111):gs10:FRB:HA

   Plasmid

   #228532

   Purpose

   A fragment of human Skp1 protein in fusion with FRB domain through a flexible glycine-serine linker.

   Depositor

   Roman Jerala

   Article

   Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

   Insert

   myc:Skp1(dC111):gs10:FRB:HA (SKP1 Synthetic, Human)
   

   Use

   Tags

   HA and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA4/TO CD16.7-h-PKD1 FLM W4108A

   Plasmid

   #83462

   Purpose

   Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and a mutation in the first Calmodulin binding domain (502)

   Depositor

   Thomas Weimbs

   Article

   Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

   Insert

   Polycystin-1 (PKD1 Human)
   

   Use

   Tags

   CD16.7

   Expression

   Mammalian

   Mutation

   Amino-acids 4078-4303, W4107A

   Promoter

   Available since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA4/TO CD16.7-h-PKD1 FLM S4144D

   Plasmid

   #83460

   Purpose

   Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and a point mutant that disrupts the second calmodulin binding domain (513)

   Depositor

   Thomas Weimbs

   Article

   Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

   Insert

   Polycystin-1 (PKD1 Human)
   

   Use

   Tags

   CD16.7

   Expression

   Mammalian

   Mutation

   Amino-acids 4078-4303, S4144D

   Promoter

   Available since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA4/TO h-PKD1 FLS L4137P-GST

    Plasmid

    #83455

    Purpose

    Human PC1-CTp30 expression cassette with a patient mutation at L4137 and a GST-tag (848)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    Polycystin-1 (PKD1 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Amino-acids 4107-4303, L4137P

    Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA4/TO h-PKD1 FLS R4150C-GST

    Plasmid

    #83454

    Purpose

    Human PC1-CTp30 expression cassette with a patient mutation at R4150 and a GST-tag (850)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    Polycystin-1 (PKD1 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Amino-acids 4107-4303, R4150C

    Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.2-Cx43-M100/125L-siResist

    Plasmid

    #49855

    Purpose

    Encodes human connexin 43 with M100 and M125 mutated to L and wobble mutations conferring resistance against an siRNA

    Depositor

    Robin Shaw

    Article

    Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

    Insert

    Connexin 43 (GJA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Methionine100 and Methionine 125 mutated to Leuci…

    Promoter

    CMV

    Available since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pCDNA3.1 hSCN5AdeltaSIV GFP Nter

    Plasmid

    #145770

    Purpose

    expresses human Nav1.5 lacking the SIV motif and tagged with GFP at its N-terminus in mammalian cells

    Depositor

    Hugues Abriel

    Article

    Petitprez et al Circ Res. 2011 Feb 4;108(3):294-304. doi

    Insert

    sodium voltage-gated channel alpha subunit 5 (SCN5A Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Stop codon at Serine 2013

    Promoter

    CMV

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(-)-C2C1-ErNaR-TS-EYFP-ES

    Plasmid

    #209851

    Purpose

    Expression of human codon-optimized light-driven sodium pump ErNaR in mammalian cells

    Depositor

    Volker Busskamp

    Article

    Podoliak et al Nat Commun. 2024 Apr 10;15(1):3119. doi:

    Insert

    light-driven sodium pump ErNaR
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA 3.3 ss-V5-hFZD4 (aa 37-537)

    Plasmid

    #115787

    Purpose

    Expresses human FZD4 with N-terminal V5-tag in mammalian cells, contains artificial signal sequence upstream of tag.

    Depositor

    Harald Junge

    Article

    Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

    Insert

    FZD4 (FZD4 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. FLAG-HA-FbxO11-ΔFbox-pcDNA3.1-

    Plasmid

    #52502

    Purpose

    expresses human FbxO11-ΔFbox in mammalian cells with FLAG-HA tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    FbxO11 (FBXO11 Human)
    

    Use

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    deletion of Fbox (deleted aa 71-116)

    Promoter

    CMV

    Available since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. FLAG-KLF12/pcDNA3.1-Neo

    Plasmid

    #81069

    Purpose

    Expression of epitope-tagged human KLF12 in mammalian cells.

    Depositor

    Julian Downward

    Article

    Godin-Heymann et al Oncogene. 2016 Jun 23;35(25):3324-34. do

    Insert

    FLAG-KLF12 (KLF12 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FLAG-HA-FbxO11-Jeff-pcDNA3.1-

    Plasmid

    #52503

    Purpose

    expresses human FbxO11-Jeff mutant in mammalian cells with FLAG-HA tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    FbxO11 (FBXO11 Human)
    

    Use

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    Q491L (Jeff)

    Promoter

    CMV

    Available since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-HA-BHLHE40(S243A/E248A)

    Plasmid

    #110155

    Purpose

    Expression of human BHLHE40 (S243A/E248A) in mammalian cells

    Depositor

    Daniele Guardavaccaro

    Article

    Kim et al Mol Cell Biol. 2014 Nov 15;34(22):4177-8

    Insert

    BHLHE40 (basic helix-loop-helix family member e40) (BHLHE40 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S243A/E248A

    Promoter

    CMV

    Available since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-Flag-NSF/S647A

    Plasmid

    #74938

    Purpose

    Mammalian expression of human NSF mutant S647A (mutation in the D2 domain of the protein)

    Depositor

    Elisa Greggio

    Article

    Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

    Insert

    NSF (NSF Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S647A (mutation in the D2 domain of the protein)

    Promoter

    CMV

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only