We narrowed to 2,138 results for: pam

1. pAAV-hSyn-dLight1.1

   Plasmid

   #111066

   Purpose

   To generate Adeno-Associated viruses for expression of dLight1.1 under synapsin promoter

   Depositor

   Lin Tian

   Article

   Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

   Has Service

   AAV1

   Insert

   dLight1.1
   

   Use

   AAV

   Tags

   Flag tag

   Expression

   Mutation

   Promoter

   Available since

   July 23, 2019

   Availability

   Academic Institutions and Nonprofits only

2. pAAV_CAG_FLEX_PKIαmut-P2A-mRuby3_WPRE

   Plasmid

   #196678

   Purpose

   AAV plasmid for mammalian Cre-dependent expression of PKIαmut-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIαmut bears the R20G/R21G mutation that renders it non-functional.

   Depositor

   Haining Zhong

   Article

   Ma et al Nature. 2022 Nov;611(7937):762-768. doi:

   Insert

   PKIαmut-P2A-mRuby3
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hsyn-GRAB_DA-mut

   Plasmid

   #140555

   Purpose

   Expresses the DA-insensitive control sensor GRAB_DA-mut in neurons

   Depositor

   Yulong Li

   Article

   Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

   Has Service

   AAV9

   Insert

   DA-insensitive sensor GRAB_DA-mut (control sensor)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   July 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-FLAG-MTOR-R2505P

   Plasmid

   #69015

   Purpose

   activating MTOR mutation

   Depositor

   David Sabatini

   Article

   Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

   Insert

   MTOR-R2505P (MTOR Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   change Arginine 2505 to Proline

   Promoter

   Available since

   Dec. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-FLAG-MTOR-E1799K

   Plasmid

   #69009

   Purpose

   activating MTOR mutation

   Depositor

   David Sabatini

   Article

   Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

   Insert

   MTOR-E1799K (MTOR Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   change Glutamate 1799 to Lysine

   Promoter

   Available since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-FLAG-MTOR-T1977R

   Plasmid

   #69011

   Purpose

   activating MTOR mutation

   Depositor

   David Sabatini

   Article

   Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

   Insert

   MTOR-T1977R (MTOR Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   change Threonine 1977 to Arginine

   Promoter

   Available since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3-FLAG-MTOR-F1888L

   Plasmid

   #69010

   Purpose

   activating MTOR mutation

   Depositor

   David Sabatini

   Article

   Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

   Insert

   MTOR-F1888L (MTOR Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   change Phenylalanine 1888 to Leucine

   Promoter

   Available since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pAAV-Ef1a-DIO-VGAT-WPRE-pA

   Plasmid

   #39320

   Depositor

   Bernardo Sabatini

   Article

   Tritsch et al Nature. 2012 Oct 11;490(7419):262-6. doi

   Insert

   Slc32a1 (Slc32a1 Mouse)
   

   Use

   AAV; R

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LRRK2-K1906M

   Plasmid

   #35946

   Depositor

   Zhaoyang Feng

   Article

   Yuan et al PLoS One. 2011;6(8):e22354. Epub 2011 Au

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pAAV-CAG-dLight1.2

    Plasmid

    #111069

    Purpose

    To generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoter

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Has Service

    AAV1

    Insert

    dLight1.2
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mutation

    Promoter

    Available since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-FLAG-MTOR-D2512H

    Plasmid

    #69017

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-D2512H (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Aspartate 2512 to Histidine

    Promoter

    Available since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

12. YFP-synDAT-S/A

    Plasmid

    #48792

    Purpose

    Expresses YFP-tagged human DAT with first 5 N-terminal serines mutated to alanine in pCIHygro

    Depositor

    Jonathan Javitch

    Article

    Fog et al Neuron. 2006 Aug 17;51(4):417-29.

    Insert

    YFP-synhDAT-S/A (SLC6A3 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Ser2, Ser4, Ser7, Ser12, and Ser13 mutated simult…

    Promoter

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. YFP-synDAT-S/D

    Plasmid

    #48793

    Purpose

    Expresses YFP-tagged human DAT with first 5 N-terminal serines mutated to aspartates in pCIHygro

    Depositor

    Jonathan Javitch

    Article

    Fog et al Neuron. 2006 Aug 17;51(4):417-29.

    Insert

    YFP-synhDAT-S/D (SLC6A3 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Ser2, Ser4, Ser7, Ser12, and Ser13 mutated simult…

    Promoter

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRK5 HA-GST-Presc-mAkt1 (S473T)

    Plasmid

    #48806

    Purpose

    expresses mutant Akt1

    Depositor

    David Sabatini

    Article

    Kang et al Science. 2013 Jul 26;341(6144):1236566.

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Tags

    HA-GST-Presc

    Expression

    Mammalian

    Mutation

    S473T

    Promoter

    CMV

    Available since

    May 2, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pAAV-TetO(3G)-GCaMP6f-WPRE

    Plasmid

    #186205

    Purpose

    Express GCaMP6f under TetO(3G)

    Depositor

    Naoshige Uchida

    Article

    Amo et al Nat Neurosci. 2022 Aug;25(8):1082-1092.

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    6xHis (N terminal on insert), T7 epitope, and Xpr…

    Expression

    Mutation

    Promoter

    TetO(3G)

    Available since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn-mCherry-SynaptoZip

    Plasmid

    #177318

    Purpose

    AAV expression of the synaptic activity-marker SynaptoZip fused to mCherry driven by human Synapsin promoter

    Depositor

    Antonio Malgaroli

    Article

    Lamanna et al Sci Rep. 2022 Jun 30;12(1):11055. doi: 1

    Insert

    mCherry-SynaptoZip (Vamp2 Rat)
    

    Use

    AAV

    Tags

    Myc and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKM33

    Plasmid

    #154167

    Purpose

    Aedes aegypti Rel2 (long isoform) with C-terminal IκB domain deleted, expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    Rel2 (long isoform)
    

    Use

    Tags

    FLAG

    Expression

    Insect

    Mutation

    C-terminal IκB domain deleted

    Promoter

    IE1 promoter and hr5 enhancer from Autographa cal…

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJWK_VD_04

    Plasmid

    #158105

    Purpose

    CaV1.3 5'UTR luciferase reporter

    Depositor

    Ted Dawson

    Article

    Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645

    Insert

    5'UTR of CACNA1D (uc003dgu.5)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJWK_VD_03

    Plasmid

    #158104

    Purpose

    CaV1.2 5'UTR luciferase reporter

    Depositor

    Ted Dawson

    Article

    Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645

    Insert

    5'UTR of CACNA1C (uc009zdu.1)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-CBE4max-SpG-P2A-EGFP (RTW4552)

    Plasmid

    #139998

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max SpCas9 variant named SpG with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…

    Promoter

    CAG

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge