281,601 results

1. pZR112_Lenti-SFFV-mCherry-2A-dCas9-VP64

   Plasmid

   #180263

   Purpose

   Lentiviral expression vector for dCas9-VP64 in mammalian cells

   Depositor

   Alexander Marson

   Article

   Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Nuclease dead Cas9

   Promoter

   SFFV

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKSE401

   Plasmid

   #62202

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Kan resistance

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Inserts

   gRNA scaffold
   zCas9
   

   Use

   Plant binary vector

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   maize codon optimized

   Promoter

   35S and AtU6-26p

   Available since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKSE401

   Plasmid

   #62202

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Kan resistance

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Inserts

   gRNA scaffold
   zCas9
   

   Use

   Plant binary vector

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   maize codon optimized

   Promoter

   35S and AtU6-26p

   Available since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-PE7-P2A-GFP

   Plasmid

   #222996

   Purpose

   Mammalian expression of SpCas9 PE7 prime editor with P2A-EGFP marker

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7-P2A-EGFP
   

   Use

   Tags

   P2A-EGFP, SV40 bpNLS, and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-DIO-ATLASsnFlp

   Plasmid

   #232352

   Purpose

   Anterograde transsynaptic tracer protein to express in presynaptic glutamatergic neurons that express Cre, Flp payload. Must use with AAV8-fDIO-Reporter viruses infected in postsynaptic cells.

   Depositor

   Don Arnold

   Article

   Rivera et al Nat Methods. 2025 May;22(5):1101-1111. d

   Insert

   ATLASsnFlp
   

   Use

   AAV

   Tags

   ALFA-Tag

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   May 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-ATF6

   Plasmid

   #32955

   Purpose

   For visualization of GFP-ATF6 in mammalian cells.

   Depositor

   Ron Prywes

   Article

   Chen et al J Biol Chem. 2002 Apr 12. 277(15):13045-

   Insert

   ATF6 (ATF6 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   M67L and M313I

   Promoter

   CMV

   Available since

   Nov. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-ATF6

   Plasmid

   #32955

   Purpose

   For visualization of GFP-ATF6 in mammalian cells.

   Depositor

   Ron Prywes

   Article

   Chen et al J Biol Chem. 2002 Apr 12. 277(15):13045-

   Insert

   ATF6 (ATF6 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   M67L and M313I

   Promoter

   CMV

   Available since

   Nov. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-FLEX-EGFPL10a (AAV5)

   Viral Prep

   #98747-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pAAV-FLEX-EGFPL10a (#98747). In addition to the viral particles, you will also receive purified pAAV-FLEX-EGFPL10a plasmid DNA. Cre-dependent EGFPL10a (EGFP-tagged ribosomal protein) expression for translating ribosome affinity purification (TRAP). These AAV preparations are suitable purity for injection into animals.

   Depositor

   Eric Schmidt, Alexander Nectow, Nathaniel Heintz

   Article

   Nectow et al Cell Rep. 2017 Apr 18;19(3):655-667. doi

   Promoter

   EF1a

   Tags

   EGFP (Cre-dependent)

   Available since

   Sept. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

9. mCherry-VAPB

   Plasmid

   #108126

   Purpose

   ER marker

   Depositor

   Gerry Hammond

   Article

   Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

   Insert

   mCherry:VAPB (VAPB Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mCherry-VAPB

    Plasmid

    #108126

    Purpose

    ER marker

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    mCherry:VAPB (VAPB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. 6xHis-SNAP-PI(4)P Probe

    Plasmid

    #211509

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol-4-Phosphate (PI(4)P) from bacteria

    Depositor

    Hannes Maib, David Murray

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    SidC (P4C)
    

    Use

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. P336-FBP_11

    Plasmid

    #139702

    Purpose

    T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing Luz

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNPGA:tAtug7, pCmYLCV:NnH3H:AtuOcs, pCmYLCV:NnHisps:tHsp
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    various

    Available since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. P336-FBP_11

    Plasmid

    #139702

    Purpose

    T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing Luz

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNPGA:tAtug7, pCmYLCV:NnH3H:AtuOcs, pCmYLCV:NnHisps:tHsp
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    various

    Available since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-CMV-LoxP-DsRed-LoxP-eGFP

    Plasmid

    #65726

    Purpose

    Functions as a genetic Cre reporter that switches from DsRed expression to eGFP expression upon presence of Cre recombinase.

    Depositor

    Jacco van Rheenen

    Article

    Zomer et al Cell. 2015 May 21;161(5):1046-57. doi: 1

    Insert

    LoxP-DsRed-LoxP-eGFP
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLV-CMV-LoxP-DsRed-LoxP-eGFP

    Plasmid

    #65726

    Purpose

    Functions as a genetic Cre reporter that switches from DsRed expression to eGFP expression upon presence of Cre recombinase.

    Depositor

    Jacco van Rheenen

    Article

    Zomer et al Cell. 2015 May 21;161(5):1046-57. doi: 1

    Insert

    LoxP-DsRed-LoxP-eGFP
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAV0286

    Plasmid

    #149333

    Purpose

    Bacterial expression of SENP_EuB protease

    Depositor

    Dirk Görlich

    Article

    Vera Rodriguez et al J Cell Biol. 2019 Jun 3;218(6):2006-2020

    Insert

    SENP_EuB protease
    

    Use

    Tags

    His14 and Tev protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAV0286

    Plasmid

    #149333

    Purpose

    Bacterial expression of SENP_EuB protease

    Depositor

    Dirk Görlich

    Article

    Vera Rodriguez et al J Cell Biol. 2019 Jun 3;218(6):2006-2020

    Insert

    SENP_EuB protease
    

    Use

    Tags

    His14 and Tev protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGP-CMV-jGCaMP8s

    Plasmid

    #162371

    Purpose

    Mammalian expression of ultrafast protein calcium sensor

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8s
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    S26M F286Y Q315H

    Promoter

    CMV

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGP-CMV-jGCaMP8s

    Plasmid

    #162371

    Purpose

    Mammalian expression of ultrafast protein calcium sensor

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8s
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    S26M F286Y Q315H

    Promoter

    CMV

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-hSyn-DIO-mCherry (AAV8)

    Viral Prep

    #50459-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-hSyn-DIO-mCherry (#50459). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-mCherry plasmid DNA. hSyn-driven, Cre-dependent mCherry-expression control. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bryan Roth

    Promoter

    Syn

    Tags

    mCherry (Cre-dependent)

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only